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Epigenetic Factors Altered in Dehisced Anther Correlated to Seed Dormancy in <i>Paris polyphylla</i>var. Yunnanensis

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Agricultural Sciences 2019年第12期10卷 1517-1533页

作者： Xia Cheng Bin Wang Linyun Liu Zhen Zhao Xia Ling Feng Zhao Dingkang Wang School of Agriculture and Life Sciences Kunming University Kunming China Kunming Dianchi Lake Environmental Protection Collaborative Research Center Kunming University Kunming China International Exchange and Cooperation Division Kunming University Kunming China

Paris polyphylla var. yunnanensis (Franch.), one of the best-known medicinal plants in China, has a dehiscent anther which physiologically work in pollination, however, the dehiscent anther always closes in response to darkness every day, and watering or raining every time. To explore this frequently closing and its unkown physiology, next-generation sequencing was performed, and the transcriptome was de novo assembled. RNA-sequencing was carried out in 15 samples including seven openning samples, four closed samples owing to darkness or watering, and tissue samples (leaf, petal, calyx, and stigma) were used for control. We obtained 72.75 GB data, assembled into 79,815 unigenes. Differentially expressed unigenes (DEGs) between opened and closed anther samples were 6231 and the DEGs between anther and control samples were 2831. Comparation between the two DEGs by KEGG enrichment showed that “plant hormone signal transduction” pathway is the most significant pathway for DEGs from closing anther vs. opening anther, and expression model of DEGs in the pathway might elicit change in germination and seed dormancy. Further examination of the action of the signal pathway on physiology showed “chromatin binding” function was prominent in “DNA binding” function of annotated DEGs between opened and closed anthers, of the 215 “chromatin binding” unigenes, 120 were involved in epigenetic silencing, and 50 of the epigenetic unigenes were directly related to germination or seed dormancy, strongly correlating anther closing to epigenetic modification and seed dormancy. These results were verified that at least three auxins involved in seed dormancy showed same expression patterns occurred in abnormal closing anther and seed embryo in Paris polyphylla var. yunnanensis. In conclusion, the information from transcriptome point out that frequent abnormal closing of dehiscent anthers possibly transfer the impact on seed dormancy, and epigenetic modification happened in closing may be the

关键词： Abnormal Closing Dehiscent Anther Epigenetic Modification Paris polyphylla var. Yunnanensis Seed Dormancy

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Comprehensive Evaluation on Impacts of Diseases on Quality of Paris polyphylla var. chinensis Based on Ultraviolet and Infrared Spectra

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Medicinal Plant 2019年第3期10卷 63-65,68页

作者： Yongxia KE Jixiu SHEN Hairong ZHONG Risha WEIZE Yunzhang XU Yuan LIU College of Pharmacy Southwest Minzu UniversityChengdu610041China State Key Laboratory of Systematic and Evolutionary Botany Institute of Botanythe Chinese Academy of SciencesBeijing100093China Ethnic Medicine Institute Southwest Minzu UniversityChengdu610041China

[Objectives] The research aimed to evaluate impacts of diseases on quality of Paris polyphylla var. chinensis. [Methods]Ultraviolet spectrophotometry was used to determine and contrast content of total saponins in root,stem and leaf from healthy and diseased plants. Infrared characteristic absorption peaks of healthy and diseased plants were found by infrared fingerprint method for comparative analysis.[Results]Total saponins content in root sample J4 of healthy plant reached 4. 89% and was the highest,while it was 0. 13% in stem sample J11 of healthy plant and was the lowest. Total saponins content in root sample B1 of diseased plant was 1. 68% and was the highest,while it was 0. 1% in stem sample B7 of diseased plant and was the lowest. In healthy and diseased plants,total saponins content in root was significantly higher than that in stem and leaf. Content of total saponins in root from healthy plant was significantly higher than that from diseased plant,and there was little difference in stem and leaf of healthy and diseased plants. Root sample Jg of healthy plant had obvious C-O characteristic vibration of polysaccharides and glycosides and absorption peak of O-H bending vibration in steroidal saponin near 1 160. 02 cm-1,while diseased plant had no obvious absorption peak. Near 861. 97 and 768. 07 cm-1,root sample Jg of healthy plant had significant C-C stretching vibration in sugar ring and characteristic absorption peak of characteristic vibration of steroidal saponin,while root sample Bg of diseased plant had no obvious characteristic peak. [Conclusions] Diseases significantly affected composition and content of total saponins and steroidal saponins from P. polyphylla var. chinensis,further affecting quality of P. polyphylla var. chinensis.

关键词： Paris polyphylla var. chinensis Ultraviolet Infrared Total saponins Quality

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Experimental Study on Analgesia and Hemostasis of Paris polyphylla,Scutellaria baicalensis and Their Compatibility

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Medicinal Plant 2020年第2期11卷 33-37页

作者： Shibo ZHAO Suoyi HUANG Zhenjing SI Lanlan GAN Shusheng HUANG Ye FU Jianjie LAN Xueying YU Yuekong CEN College of Clinical Medicine Youjiang Medical University for Nationalities Baise 533000 China College of Pharmacy Youjiang Medical University for Nationalities Baise 533000 China Key Laboratory of Guangxi Universities on National Medicine in Youjiang River Youjiang Medical University for Nationalities Baise 533000 China

[Objectives]The analgesic and hemostatic effects of Paris polyphylla,Scutellaria baicalensis,and their compatibility were studied.[Methods](i)In the experiment on the writhing of mice induced by acetic acid,60 Kunming SPF mice were randomly selected,and were randomly divided into 6 groups according to the male-to-female ratio of 1∶1,including blank control group,*** group,*** group,and three *** and *** compatibility groups(with the ratio of 1∶2,1∶1 and 1∶2).There were 10 mice in each *** the experimental groups,the mice were given 1 g/mL Chinese medicine extract according to a dose of 20 mL/*** the control group,the mice were given 0.9%normal saline in equal *** was performed one time every 24 h and lasted for 14 d.1 h after the gavage on the 14th day,they were given 0.5%glacial acetic acid solution via intraperitoneal injection.(ii)In the hot plate experiment,60 Kunming SPF mice were selected,and the ratio of male to female,grouping,administration and gavage were the same as those of the glacial acetic acid-induced writhing *** was lasted for 14 d.1 h after the gavage on the 14th day,the mice were placed on a hot plate apparatus at(55±0.5)℃,and the time of licking hindfoot was measured.(iii)The hemostatic effect was explored through three experimental methods of tail hemostasis,femoral artery hemostasis and liver hemostasis.60 Kunming SPF mice were taken in each of the three experiments,and the male-female ratio and grouping were the same as *** powder was covered on the surface of the wound sites,and the bleeding was *** hemostatic time was recorded,and the hemostatic time was recorded as 3 min if it exceeded 3 min.[Results](i)In the acetic acid-induced writhing experiment,compared with the blank control group,the pain of mice in the experimental groups was *** them,*** group,*** group,compatibility 1∶2 group,compatibility 1∶1 group,and compatibilit

关键词： Paris polyphylla Scutellaria baicalensis Compatibility Analgesic effect Hemostatic effect

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高通量RNA-seq技术开发梵净山重楼SSR标记

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分子植物育种 2019年第18期17卷 6059-6065页

作者：王海明阮成江梁玖华韩平闫蕊董旭刘祾悦帅小春刘贺贺大连民族大学资源植物研究所生物技术与资源利用教育部重点实验室大连116600 铜仁科学院梵净山生物资源研究所铜仁554300

梵净山重楼(Paris polyphylla Smith var. chinensis)是中国重要的中草药之一。本研究利用MISA软件从重楼块根高通量转录组数据库的38 802条Unigenes中共搜索挖掘出6 333个SSR标记。66.76%的二核苷酸重复是这些SSR标记的主要重复类型,... 详细信息

梵净山重楼(Paris polyphylla Smith var. chinensis)是中国重要的中草药之一。本研究利用MISA软件从重楼块根高通量转录组数据库的38 802条Unigenes中共搜索挖掘出6 333个SSR标记。66.76%的二核苷酸重复是这些SSR标记的主要重复类型,然后是18.77%的三核苷酸重复、11.50%的单核酸苷酸重复及2.97%的四核苷酸、五核苷酸和六核苷酸重复。在所有被功能注释的Unigenes中,481条Unigenes涉及调控重楼皂苷、黄酮、种子萌发和种子生长发育,从这些Unigenes共搜索挖掘到90个SSR标记。通过对这90个SSR标记的有效性验证,获得具有清晰扩增条带的SSR标记20对。以选取的14份重楼种质为材料,对扩增出清晰条带的20对SSR标记进行多态性检测后,获得16对具有多态性的SSR标记引物。引物多态性信息含量指数(polymorphism information content, PIC)介于0.067~0.561,平均值为0.293;14个重楼种质聚类在两个不同类群。本研究结果可为梵净山重楼种质资源鉴定、遗传多样性分析和分子标记辅助选择育种提供SSR标记。

关键词：重楼(Paris polyphylla Smith var. chinensis) RNA-seq SSR标记

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超声波对滇重楼种子萌发能力的影响

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湖北农业科学 2012年第1期51卷 114-116页

作者：周浓李自勤姜北张采琼杨绍群大理学院药学院云南大理671000

研究超声波处理时间、温度、功率对滇重楼(Paris polyphylla ***)种子萌发的影响,寻找其萌发的最佳条件。通过室内常规发芽试验,统计滇重楼种子在不同超声波处理时间、温度、功率条件下的发芽率和发芽指数。结果表明,滇重楼种子经适当... 详细信息

研究超声波处理时间、温度、功率对滇重楼(Paris polyphylla ***)种子萌发的影响,寻找其萌发的最佳条件。通过室内常规发芽试验,统计滇重楼种子在不同超声波处理时间、温度、功率条件下的发芽率和发芽指数。结果表明,滇重楼种子经适当的超声波处理,缩短了萌发时间,提高了发芽率和发芽指数。40 min、50℃、200 W的超声波处理条件最适宜于滇重楼种子萌发。

关键词：滇重楼(Paris polyphylla var.yunnanensis) 种子超声波萌发率萌发指数

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多叶重楼遗传多样性的RAPD分析

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生物多样性 2004年第5期12卷 517-522页

作者：张金渝虞泓张时刚丁长春云南大学生命科学院生态遗传学实验室云南英茂生物技术实验室昆明650212

应用RAPD技术检测了多叶重楼 (Parispolyphylla) 2个变种 4个居群的遗传多样性 ,并与 1个凌云重楼 (P .cronquistii)居群进行了比较。选择的 16个随机引物在 5个居群中共检测到 2 4 6个多态位点。在居群水平上 ,滇重楼 2个居群的多态位... 详细信息

应用RAPD技术检测了多叶重楼 (Parispolyphylla) 2个变种 4个居群的遗传多样性 ,并与 1个凌云重楼 (P .cronquistii)居群进行了比较。选择的 16个随机引物在 5个居群中共检测到 2 4 6个多态位点。在居群水平上 ,滇重楼 2个居群的多态位点百分比 (PPB)分别为 5 7.4 3%和 5 4 .6 7% ,Shannon指数分别为 0 .30 80和 0 .2 830 ;七叶一枝花2个居群的PPB分别为 5 6 .33%和 5 7.75 % ,Shannon指数分别为 0 .30 80和 0 .32 93。在变种水平上 ,滇重楼的PPB为75 .14 % ,Shannon指数为 0 .392 2 ,遗传分化系数 (Gst)为 0 .30 85 ;七叶一枝花的PPB为 80 .31% ,Shannon指数为 0 .3992 ,遗传分化系数 (Gst)为 0 .372 6 ;在种的水平PPB达 92 .0 5 % ,遗传分化系数Gst达 0 .5 15 1。聚类分析显示滇重楼和七叶一枝花有较近的亲缘关系 ,而与凌云重楼遗传距离较远。此结果从分子水平上支持了过去将滇重楼和七叶一枝花划分为 1个种下

关键词： Paris polyphylla RAPD 遗传多样性居群遗传分化

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华重楼愈伤组织培养及薯蓣皂苷含量测定

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湖北农业科学 2014年第8期53卷 1936-1940页

作者：王跃华陈燕张珏张红玉游亚莉田孟良成都大学生物产业学院成都610106 四川金土地中药材种植集团有限公司成都611130 四川农业大学新农村发展研究院四川雅安625014

以华重楼[Paris polyphylla Smith ***(Franch.)Hara]植物多种器官为外植体,经不同消毒处理和培养基诱导培养后,观察其愈伤组织的诱导生长情况;并以TTC法检测诱导产生的愈伤组织中的薯蓣皂苷含量。结果表明,华重楼的隐芽、内层芽鞘、根... 详细信息

以华重楼[Paris polyphylla Smith ***(Franch.)Hara]植物多种器官为外植体,经不同消毒处理和培养基诱导培养后,观察其愈伤组织的诱导生长情况;并以TTC法检测诱导产生的愈伤组织中的薯蓣皂苷含量。结果表明,华重楼的隐芽、内层芽鞘、根茎、预处理后根茎和子房外植体经诱导培养后,均可不同程度地产生愈伤组织;其中以预处理后根茎外植体产生的愈伤组织生长速度最快,诱导率也最高,为33.5%。经检测结果显示,多次继代培养的华重楼愈伤组织中含有与华重楼对照药材和薯蓣皂苷对照品相同的薯蓣皂苷或其类似物。

关键词：华重楼[Paris polyphylla Smith vat chinensis(Franch )Hara] 外植体诱导愈伤组织

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滇重楼最佳授粉期的研究

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安徽农业科学 2012年第12期40卷 7083-7084页

作者：曾卫民赵庭周云南民族大学科技处云南昆明650500 云南白药集团中药材优质种源繁育有限责任公司云南武定651600

[目的]优选滇重楼的最佳授粉期。[方法]采用TTC法测定花粉的活力,并采用联苯胺-过氧化氢法测定柱头可授性,根据研究滇重楼花粉活力变化规律及柱头可授性优选滇重楼的最佳授粉期。[结果]滇重楼花粉囊开裂时活力最高为68.2%,前2 d内,花粉... 详细信息

[目的]优选滇重楼的最佳授粉期。[方法]采用TTC法测定花粉的活力,并采用联苯胺-过氧化氢法测定柱头可授性,根据研究滇重楼花粉活力变化规律及柱头可授性优选滇重楼的最佳授粉期。[结果]滇重楼花粉囊开裂时活力最高为68.2%,前2 d内,花粉活力下降不明显。第4天开始,活力明显下降,但仍然具有一定的活性。柱头的活性在花粉囊开裂后5 d内最强,滇重楼的授粉应选择在这5 d内进行。[结论]该研究优选了滇重楼的最佳授粉期,为滇重楼的进一步开发利用提供了依据。

关键词：滇重楼(Paris polyphylla Smith var.yunnanensis(Franch.)Hand.-Mazz.) 最佳授粉期

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Study on Phenological Phase of Three Wild Plants in Limestone Mountainous Area of Chongqing City in China

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Journal of Landscape Research 2011年第4期3卷 42-44页

作者：卢高峰陈晓德徐洪辉李朝政西南大学生命科学学院重庆400715 三峡库区生态环境教育部重点实验室重庆400715

The paper had studied phenological phase of three wild plants in Haishi Park at limestone mountainous area of Chongqing which were Berchemia polyphylla ***,Caesalpinia decapetala and Bauhinia glauca *** were selected based on terrain,and it had selected 30 plants of Berchemia polyphylla *** and Caesalpinia decapetala and 7 plants of Bauhinia glauca *** which were different in shape but similar in *** point observation was conducted on them and observation results were *** had explored ornamental value of the three wild plants and identified their corresponding ornamental periods,so as to provide scientific reference and suggestions for gardening and application of the three wild plants.

关键词： Berchemia polyphylla var.leioclada Caesalpinia decapetala Bauhinia glauca subsp.hupehana Ornamental phenology Gardening application

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