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Evaluation of the Behaviour of Wrinkles Fibroblasts and Normal Aged Fibroblasts in the Presence of poly-l-lactic acid

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Journal of Cosmetics, Dermatological Sciences and Applications 2012年第1期2卷 20-27页

作者： Carol Courderot-Masuyer Sophie Robin Helene Tauzin Philippe Humbert Bioexigence SARl Espace LafayetteBesanconFrance Department of Dermatology Laboratory of Cutaneous BiologyBesançonFrance Clinical Investigation Center BesanconFrance University of Franche-Comte BesanconFrance INSERM UMR1098 SFR FED 4234 IBCTBesanconFrance

Background: Wrinkles are characterized by changes in the organization and structure of the dermis. Human wrinkle fibroblasts (WF) have a different functional behaviour in comparison with normal-aged fibroblasts (NF). Decreases in migration capacities and collagen I synthesis are observed. Mitochondrial function is impaired with an increase in lactate production during aging. Sculptra? (poly-l-lactic acid: PllA), a biodegradable synthetic polymer, is used for subcutaneous volume restoration. Thus we decided to investigate different fibroblast functions when placed in contact with PllA. Objectives: The potential of PllA to compensate for the reduction of metabolic activity, to restore the migration capacity of WF and to inhibit the lactate production, was investigated and compared to NF. Methods: Two different skin samples were used from each of the three women’s facelift (one inside a face wrinkle and one from normal aged skin). Collagen I, lactate productions and proliferation capacities were investigated on monolayer cultures. Migration properties were evaluated using three-dimensional collagen lattices. Results: PllA increased collagen I synthesis, restored migration capacities and tended to decrease lactate production in WF, whereas PPlA stimulated proliferation in NF and tended to improve the migration of NF. Conclusion: These results suggested that PllA from Sculptra? acted as a stimulus for collagen production in WF and that it is suitable for correcting skin depressions, such as wrinkles.

关键词： Wrinkles poly-l-lactic acid Collagen Fibroblast

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Dynamic culture of a thermosensitive collagen hydrogel as an extracellular matrix improves the construction of tissue-engineered peripheral nerve

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Neural Regeneration Research 2014年第14期9卷 1371-1378页

作者： lanfeng Huang Rui li Wanguo liu Jin Dai Zhenwu Du Xiaonan Wang Jianchao Ma Jinsong Zhao Department of Joint Surgery Orthopedics Hospital of the Second Hospital of Jilin University Centre of Hand & Foot Surgery and Reparative & Reconstructive Surgery Orthopedics Hospital of the Second Hospital of Jilin University Department of Orthopedics Surgery the Third Hospital of Jilin University Institute of Orthopedics the Second Hospital of Jilin University Department of Ophthalmology the Second Hospital of Jilin University

Tissue engineering technologies offer new treatment strategies for the repair of peripheral nerve injury, hut cell loss between seeding and adhesion to the scaffold remains inevitable. A thermosensitive collagen hydrogel was used as an extracellular matrix in this study and combined with bone marrow mesenchymal stem cells to construct tissue-engineered peripheral nerve composites in vitro. Dynamic culture was performed at an oscillating frequency of 0.5 Hz and 35° swing angle above and below the horizontal plane. The results demonstrated that bone marrow mesenchymal stem cells formed membrane-like structures around the poly-l-lactic acid scaffolds and exhibited regular alignment on the composite surface. Collagen was used to fill in the pores, and seeded cells adhered onto the poly-l-lactic acid fibers. The DNA content of the bone marrow mesenchymal stem cells was higher in the composites constructed with a thermosensitive collagen hydrogel compared with that in collagen I scaffold controls. The cellular DNA content was also higher in the thermosensitive collagen hydrogel composites constructed with the thermosensitive collagen hydrogel in dynamic culture than that in static culture. These results indicate that tissue-engineered composites formed with thermosensitive collagen hydrogel in dynamic culture can maintain larger numbers of seeded cells by avoiding cell loss during the initial adhe-sion stage. Moreover, seeded cells were distributed throughout the material.

关键词： nerve regeneration peripheral nerve biomaterials extracellular matrix tissueengineering nerve scaffold bone marrow mesenchymal stem cells thermosensitive collagen hydrogel poly-l-lactic acid dynamic culture NSFC grant neural regeneration

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THE SYNTHESIS AND CHARACTERIZATION OFpoly-l-lACTIDE USED FOR THE INTERNAlFIXATION OF BONE FRACTURE

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Chinese Journal of Reactive polymers 1998年第2期7卷 1-6页

作者： Zhang Zhengpu lu ge Wu Zhizhong He Binglin State key laboratory of Functional polymer Materials for Adsorption and Separation Institute of Polymer Chemistry Nankai UniversityTianjin 300071

A high Mw of PllA was synthesized, it intended to be used in the manufacture ofbioabsorbable screw applied in bone fracture internal fixation. The optical reactionconditions have been discussed.

关键词： poly-l-lactic acid Bioabsorbable Bone fracture internal fixation

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