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Targeting of pollen tubes to Ovules Is Dependent on Nitric Oxide （NO） Signaling

Molecular Plant 2008年第4期1卷 703-714页

作者： Ana Margarida Prado Renato Colaco Nuno Moreno Ana Catarina Silva Jose A. Feijo Instituto Gulbenkian de Ciencla Centro de Blologia do Desenvolvimento PT-2780-156 Oeiras Portugal Universidade de Lisboa Faculdade de Clencias Dept Biologia Vegetal Campo Grande Ed.C2. PT-1749-016 Lisboa Portugal

The guidance signals that drive pollen tube navigation inside the pistil and micropyle targeting are still, to a great extent, unknown. Previous studies in vitro showed that nitric oxide （NO） works as a negative chemotropic cue for pollen tube growth in lily （Lilium Iongiflorum）. Furthermore, Arabidopsis thaliana Atnosl mutant plants, which show de- fective NO production, have reduced fertility. Here, we focus in the role of NO in the process of pollen-pistil communication, using Arabidopsis in-vivo and lily semi-vivo assays. Cross-pollination between wild-type and Atnosl plants shows that the mutation affects the pistil tissues in a way that is compatible with abnormal pollen tube guidance. Moreover, DAF- 2DA staining for NO in kanadi floral mutants showed the presence of NO in an asymmetric restricted area around the micropyle. The pollen-pistil interaction transcriptome indicates a time-course-specific modulation of transcripts of AtNOS1 and two Nitrate Reductases （nrl and nr2）, which collectively are thought to trigger a putative NO signaling pathway. Semivivo assays with isolated ovules and lily pollen further showed that NO is necessary for micropyle targeting to occur. This evidence is supported by CPTIO treatment with subsequent formation of balloon tips in pollen tubes facing ovules. Activation of calcium influx in pollen tubes partially rescued normal pollen tube morphology, suggesting that this pathway is also dependent on Ca^2＋ signaling. A role of NO in modulating Ca^2＋ signaling was further substantiated by direct imaging the cytosolic free Ca^2＋ concentration during NO-induced re-orientation, where two peaks of Ca^2＋ occur--one during the slowdown/stop response, the second during re-orientation and growth resumption. Taken together, these results provide evidence for the participation of NO signaling events during pollen-pistil interaction. Of special relevance, NO seems to directly affect the targeting of pollen tubes to the ovule＇s micropyle by modu

关键词： pollen tubes NO fertilization guidance calcium signaling

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MALE GAMETOPHYTE DEFECTIVE 2,Encoding a Sialyltransferase-like Protein,is Required for Normal pollen Germination and pollen Tube Growth in Arabidopsis

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Journal of Integrative Plant Biology 2010年第9期52卷 829-843页

作者： Yi Deng Wei Wang Wen-Qing Li Chuan Xia Hong-Ze Liao Xue-Qin Zhang De Ye State Key-Laboratory of Pla-nt Physiology and Biochemistry College of Biological Sciences China Agricultural University Beijing 100193 China

Sialyltransferases （SlaTs） exist widely in vertebrates and play important roles in a variety of biological processes. In plants, several genes have also been identified to encode the proteins that share homology with the vertebrate SlaTs. However, very little is known about their functions in plants. Here we report the identification and characterization of a novel Arabidopsis gene, MALE GAMETOPHYTE DEFECTIVE 2 （MGP2） that encodes a sialyltransferase-like protein. MGP2 was expressed in all tissues including pollen grains and pollen tubes. The MGP2 protein was targeted to Golgi apparatus. Knockout of MGP2 significantly inhibited the pollen germination and retarded pollen tube growth in vitro and in vivo, but did not affect female gametophytic functions. These results suggest that the sialyltransferase-like protein MGP2 is important for normal pollen germination and pollen tube growth, giving a novel insight into the biological roles of the *** proteins in plants.

关键词： Plants Planting Proteins Protein products Imports Genes Vertebrates pollen tubes Growth Germinability

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Formin controls polarized pollen tube growth by nucleating actin assembly for the formation of actin cables

Formin controls polarized pollen tube growth by nucleating a...

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2009中国植物学会植物细胞生物学学术年会

作者： Shanjin Huang~* Institute of Botany,the Chinese Academy of Sciences,Beijing 100093

Cytoplasmic actin bundles,also known as actin cables,are the most prominent actin structures in plant cells,but the molecular mechanism underlying the formation of these actin cables is *** function of these actin cab... 详细信息

关键词： FH1-FH2 domains pollen tubes polarity

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