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Cytological and Proteomic Analysis of Ginkgo biloba pollen intine

Horticultural Plant Journal 2020年第4期6卷 257-266页

作者： Weixing Li Yunling Ye Fangmei Cheng Yan Lu Biao Jin Li Wang College of Horticulture and Plant Protection Yangzhou UniversityYangzhou 225009China Suqian Academy of Protected Horticulture Nanjing Agricultural UniversitySuqianJiangsu 223800China Instrumental Analysis Center Yangzhou UniversityYangzhou 225009China

The pollen intine plays important roles in pollen germination and tube growth,but related information in Ginkgo biloba remains *** isolated and obtained de-exined pollen from *** fluorescent probes,we observed the strongest cellulose fluorescence in the pollen ***-esterified pectin immunolabeled with JIM5 was present throughout the entire cell wall,whereas esterified pectin recognized by the monoclonal antibody JIM7 was concentrated in some *** staining with aniline blue was observed across the entire surface of the pollen *** results were confirmed by Fourier Transform InfraRed(FTIR)*** also used proteomic approaches to identify different proteins between mature and de-exined pollen(48h after hydration)in *** on mass spectrometry,de-exined pollen had more proteins than mature pollen,including calmodulin,serine hydroxymethyltransferase,β-galactosidase 6,and class IV *** to Gene Ontology(GO)analysis,the differentially expressed proteins were mainly associated with transportation,defense reaction,sugar metabolism,energy metabolism,signal transduction,and cell wall *** findings suggest that most proteins involved in pollen germination and pollen tube growth are synthesized during pollen hydration,indicating the important role of pollen hydration in the reproductive process of ***.

关键词： Ginkgo biloba pollen intine cytological analysis proteomics mass spectrometry

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The fasciclin-like arabinogalactan protein gene,FLA3,is involved in microspore development of Arabidopsis

The fasciclin-like arabinogalactan protein gene,FLA3,is invo...

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中国植物学会植物细胞生物学2010年学术年会

作者： Jun Li,Miao Yu,Ling-Ling Geng,Jie Zhao~* Key Laboratory of the Ministry of Education for Plant Developmental Biology,College of Life Sciences, Wuhan University,Wuhan 430072

Arabinogalactan proteins are widely distributed in plant tissues and cells,and may function in the growth and development of higher *** our knowledge,no direct evidence currently addresses the involvement of the fasciclin-like arabinogalactan protein(FLA) in Arabidopsis sexual reproduction. In this study,Arabidopsis FLA3 was found to be specifically expressed in pollen grains and its tubes. Subcellular localization showed that FLA3 anchors tightly to the plasma membrane,and its glycosylphosphatidylinositol(GPI) anchor may affect its ***3-RNAi(RNA inteference) transgenic plants had approximately 50%abnormal pollen grains(including shrunken and wrinkled phenotypes) which lacked *** observations revealed that pollen abortion occurred during the transition from uninucleate microspores to bicellular pollens,with abnormal cellulose distribution seen by calcofluor white *** electron microscopy showed that the basic structure of the exine layer in aberrant pollen was normal,but the intine layer appeared to have some *** together,these results suggest that the FLA3 is involved in microspore development and may affect pollen intine formation possibly by participating in cellulose *** FLA3-overexpressing transgenic plants,defective stamen filament elongation and reduced female fertility led to short siliques with low seed set,which suggested that ectopic expression of FLA3 in tissues may reduce or disrupt cell growth and then result in defects throughout the plant.

关键词： Arabidopsis arabinogalactan proteins glycosylphosphatidylinositol(GPI) anchor microspore development pollen intine sterility

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