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Recent progress in nanomaterial-enhanced fluorescence polarization/anisotropy sensors

Chinese Chemical Letters 2019年第9期30卷 1575-1580页

作者： Jia Chen Juewen Liu Xingguo Chen Hongdeng Qiu CAS Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province Lanzhou Institute of Chemical Physics Chinese Academy of Sciences Lanzhou 730000 China University of Chinese Academy of Sciences Chinese Academy of Sciences Beijing 10039 China Department of Chemistry Waterloo Institute for Nanotechnology University of Waterloo Waterloo Ontario N2L 3G1 Canada State Key Laboratory of Applied Organic Chemistry Lanzhou University Lanzhou 730000 China

As a promising signaling transduction approach, fluorescence polarization(FP)/fluorescence anisotropy(FA), provides a powerful quantitative tool for the rotational motion of fluorescently labeled molecules in chemical or biological homogeneous systems. Unlike fluorescence intensity, FP/FA is almost independent the concentration or quantum of fluorophores, but they are highly dependent on the size or molecular weight of the molecules or materials attached to fluorophores. Recently, significant progress in FP/FA was made, due to the introduction of some nanomaterials as FP/FA enhancers. The detection sensitivity is thus greatly improved by using nanomaterials as FP/FA enhancers, and nanomaterial-based FP/FA is currently used successfully in immunoassay, and analysis of protein, nucleic acid, small molecule and metal ***-based FP/FA provides a new kind of strategy to design fluorescent sensors and establishes innovative analytical methods. In this review, we summarize the scientific publications in the field of FP/FA sensor in recent five years, and first introduce the recent progress of FP/FA sensor based on nanomaterial. Subsequently, the various analytical applications of FP/FA based on nanomaterial are discussed. Finally, we provide perspectives on the current challenges and future prospects in this promising field.

关键词： Fluorescence polarization/anisotropy Nanomaterials Sensor Recent progress Application

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