检索结果-南通市图书馆

pluripotent stem cells secrete Activin A to improve their epiblast competency after injection into recipient embryos

引用

Protein & Cell 2018年第8期9卷 717-728页

作者： Jinzhu Xiang Suying Cao Liang Zhong Hanning Wang Yangli Pei Qingqing Wei Bingqiang Wen Haiyuan Mu Shaopeng Zhang Liang Yue Genhua Yue Bing Lim Jianyong Han State Key Laboratory for Agrobiotechnology College of Biological Sciences China Agricultural University Beijing 100193 China Animal Science and Technology College Beijing University of Agriculture Beijing 102206 China Temasek Life Sciences Laboratory National University of Singapore Singapore 117604 Singapore stem Cell and Developmental Biology Genome Institute of Singapore Singapore 138672 Singapore Beijing Advanced Innovation Center for Food Nutrition and Human Health China Agricultural University Beijing 100193 China State Key Laboratory of Animal Nutrition Institute of Animal Sciences Chinese Academy of Agricultural Sciences Beijing 100193 China

It is not fully clear why there is a higher contribution of pluripotent stem cells （PSCs） to the chimera produced by injection of PSCs into 4-cell or 8-cell stage embryos compared with blastocyst injection. Here, we show that not only embryonic stem cells （ESCs） but also induced pluripotent stem cells （iPSCs） can generate F0 nearly 100% donor cell-derived mice by 4-cell stage embryo injection, and the approach has a ＂dose effect＂. Through an analysis of the PSC-secreted proteins, Activin A was found to impede epiblast （EPI） lineage development while promoting trophectoderm （TE） differentiation, resulting in replacement of the EPI lineage of host embryos with PSCs. Interestingly, the injection of ESCs into blastocysts cultured with Activin A （cultured from 4-cell stage to early blastocyst at E3.5） could increase the contribution of ESCs to the chimera. The results indicated that PSCs secrete protein Activin A to improvetheir EPI competency after injection into recipient embryos through influencing the development of mouse early embryos. This result is useful for optimizing the chimera production system and for a deep understand- ing of PSCs effects on early embryo development.

关键词： pluripotent stem cells 4-cell embryoinjection secreted proteins Activin A chimeric mice

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Functional microglia derived from human pluripotent stem cells empower retinal organs

引用

Science China(Life Sciences) 2022年第6期65卷 1057-1071页

作者： Mei-Ling Gao Xiao Zhang Fang Han Jia Xu Si-Jian Yu Kangxin Jin Zi-Bing Jin Institute of stem Cell Research The Eye HospitalWenzhou Medical UniversityWenzhou 325027China Beijing Institute of Ophthalmology Beijing Tongren Eye CenterBeijing Tongren HospitalCapital Medical UniversityBeijing Ophthalmology&Visual Sciences Key LaboratoryBeijing 100730China

Microglia are known to play essential roles in the development,progression and treatment of diverse neurodegenerative diseases in the central nervous system,including the retina,brain and spinal cord.Recently,brain-induced microglia-like cells(iMGs)have been generated from human pluripotent stem cells(hPSCs);however,retinal microglia have yet to be developed in vitro.In this study,by mimicking in vivo microglial development,we established a simplified approach to differentiate hPSCs into high purity(>90%)iMGs.The iMGs express microglia-specific markers,release cytokines upon stimulation,and are capable of phagocytizing bacteria.When co-cultured with three-dimensional human retinal organoids(hROs),iMGs migrated into the hROs,tended to differentiate into resident retinal microglia,and simultaneously induced apoptosis in some neural cells.Notably,the resident i MGs in the hROs formed sparse web-like structures beneath the photoreceptor cell layer,resembling microglia's orientation in human retina.In conclusion,we developed a simplified and efficient method to generate microglia from human pluripotent stem cells,and we report the first derivation of retinaresident microglia in vitro,providing a new source of human retinal microglia for developmental and disease studies and regenerative therapeutics.

关键词： microglia retinal organoid pluripotent stem cells differentiation human induced reprogramming

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Non-human primate pluripotent stem cells for the preclinical testing of regenerative therapies

引用

Neural Regeneration Research 2022年第9期17卷 1867-1874页

作者： Ignacio Rodríguez-Polo Rüdiger Behr Research Platform Degenerative Diseases German Primate Center-Leibniz Institute for Primate ResearchGöttingenGermany German Center for Cardiovascular Research(DZHK) Partner Site GöttingenGöttingenGermany

Non-human primates play a key role in the preclinical validation of pluripotent stem cell-based cell replacement therapies.pluripotent stem cells used as advanced therapy medical products boost the possibility to regenerate tissues and organs affected by degenerative diseases.Therefore,the methods to derive human induced pluripotent stem cell and embryonic stem cell lines following clinical standards have quickly developed in the last 15 years.For the preclinical validation of cell replacement therapies in non-human primates,it is necessary to generate non-human primate pluripotent stem cell with a homologous quality to their human counterparts.However,pluripotent stem cell technologies have developed at a slower pace in non-human primates in comparison with human cell systems.In recent years,however,relevant progress has also been made with non-human primate pluripotent stem cells.This review provides a systematic overview of the progress and remaining challenges for the generation of non-human primate induced pluripotent stem cells/embryonic stem cells for the preclinical testing and validation of cell replacement therapies.We focus on the critical domains of(1)reprogramming and embryonic stem cell line derivation,(2)cell line maintenance and characterization and,(3)application of non-human primate pluripotent stem cells in the context of selected preclinical studies to treat cardiovascular and neurodegenerative disorders performed in non-human primates.

关键词： embryonic stem cells induced pluripotent stem cells non-human primates pluripotent stem cells preclinical regeneration reprogramming translational research

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Human embryos in a dish - modeling early embryonic development with pluripotent stem cells

引用

Cell Regeneration 2022年第1期11卷 40-43页

作者： Xiukun Wang Guang Hu Epigenetics and stem Cell Biology Laboratory National Institute of Environmental Health Sciences Research Triangle ParkDurhamNC 27709USA

stem cell-based embryo models present new opportunities to study early embryonic development.In a recent study,Kagawa et al.identified an approach to create human pluripotent stem cell-based blastoids that resemble the human blastocysts.These blastoids efficiently generated analogs of the EPI,TE,PrE lineages with transcriptomes highly similar to those found in vivo.Furthermore,the formation of these lineages followed the same sequence and pace of blas-tocyst development,and was also dependent on the same pathways required for lineage specification.Finally,the blastoids were capable of attaching to stimulated endometrial cells to mimic the process of implantation.While more comprehensive analysis is needed to confirm its validity and usefulness,this new blastoid system presents the latest development in the attempt to model early human embryogenesis in vitro.

关键词： pluripotent stem cells stem cell-based embryo models Blastoid

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Soft substrate and decreased cytoskeleton contractility promote coupling and morphology maintenance of pluripotent stem cells

引用

Acta Mechanica Sinica 2021年第10期37卷 1520-1529,I0002页

作者： Meihong Lu Jialing Cao Yuanyuan Zhai Peng Zhao Jie Yao Yan Gong Jing Du Yubo Fan Key Laboratory for Biomechanics and Mechanobiology of the Ministry of Education Institute of Nanotechnology for Single Cell AnalysisBeijing Advanced Innovation Center for Biomedical EngineeringSchool of Biological Science and Medical EngineeringBeihang UniversityBeijing100083China Institute of Biomechanics and Medical Engineering Department of Mechanical EngineeringSchool of AerospaceTsinghua UniversityBeijing100084China Sino-French Engineer School Beihang UniversityBeijing100083China School of Material Science&Engineering Beijing Institute of Fashion TechnologyBeijing100029China

The mechanical microenvironment affects the morphology and mechanical properties of cells, and it also plays an important role in cell functions. pluripotent stem cells (PSCs) grow as multicellular colonies, and the coupling effects of cell–cell and cell-extracellular matrix interactions are complex but necessary for the formations and functions of tissues. This paper uses the finite element method to establish a three-dimensional calculation model of a pair of deformed PSCs in contact with each other and considers the growth and depolymerization of actin filaments. Then the effects of substrate stiffness on the morphology of cells and nuclei and the rearrangement of cytoskeleton are demonstrated. As the substrate becomes softer, the nuclei become loose and round, and the actin filaments will be assembled at a lower level, which could promote the formation of compacted cell colony while having a positive effect on maintaining pluripotency and inducing reprogramming efficiency. In addition, stronger activation of cytoskeleton contractility will compress the cytoplasm and nuclei. The cell mechanics model proposed in this paper provides a strategy for studying the cell morphology and cytoskeleton response of the two-cell system under different biophysical stimuli, and also lays the foundation for the further research on more mechanical factors of cell pluripotency.

关键词： pluripotent stem cells Morphology Cell model Mechanical stimuli

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

pluripotent stem cells exhibiting similar characteristics can be isolated from human fetal bone marrow, heart, liver, muscle, lung, derma, kidney, and fat

引用

Frontiers of Medicine 2007年第2期1卷 185-191页

作者： FANG Baijun SONG Yongping ZHAO Chunhua SHI Mingxia LIN Quande Henan Tumor Hospital Henan Institute of HematologyZhengzhou 450008China Institute of Basic Medical Sciences Center for Tissue EngineeringChinese Academy of Medical ScienceBeijing 100005China

Previously,we reported that a cell population derived from human fetal bone marrow(BM),termed here Flk1+CD34−postembryonic pluripotent stem cells(PPSCs)that have the characteristics of mesenchymal stem cells(MSCs),could differentiate into ectodermal,endodermal and mesodermal cell types at the single cell level in vitro,and that these cells could also differentiate into the epithelium of liver,lung,gut,as well as the hematopoietic and endothelial lineages after transplantion into irradiated non-obese diabetic/severe combined immunodeficient(NOD/SCID)mice.In this study,we further isolated pluripotent stem cells from human fetal heart,liver,muscle,lung,derma,kidney,and fat and then analyzed the characteristics and function of these stem cells.It was found that the phenotype of the culture-expanded pluripotent stem cells from different fetal tissues was similar to BM-derived Flk1^(+)CD34^(−)PPSCs,i.e.Flk1 and CD44 positive,GlyA,CD34,CD45,class I-HLA and HLA-DR negative.Morphologically,these cells were fibroblast-like and the doubling time was about 30 h.More importantly,culture-expanded pluripotent stem cells from all these fetal tissues were able to differentiate into cells with morphologic and phenotypic characteristics of adipocytes,osteocytes,neurons,glial cells and hepatocytes.These pluripotent stem cells with characteristics similar to fetal BM-derived Flk1^(+)CD34^(−)PPSCs can be selected and cultured from tissues other than the BM.This phenomenon may help explain the“stem cell plasticity”found in multiple human tissues.In addition,as fetal BM-derived Flk1^(+)CD34^(−)PPSCs,these pluripotent stem cells from different fetal tissues had the capacity for self-renewal and multi-lineage differentiation even after being expanded for more than 40 population doublings in vitro.Thus,they may be an ideal source of stem cells for treatment of inherited or degenerative diseases.

关键词： pluripotent stem cells fetal plasticity

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Two-step protocol for regeneration of immunocompetent T cells from mouse pluripotent stem cells

引用

Blood Science 2020年第3期2卷 79-88页

作者： Tongjie Wang Cui Lv Fangxiao Hu Lijuan Liu Jinyong Wang State Key Laboratory of Experimental Hematology CAS Key Laboratory of Regenerative BiologyGuangzhou Institutes of Biomedicine and HealthChinese Academy of SciencesGuangzhou510530China Guangdong Provincial Key Laboratory of stem Cell and Regenerative Medicine Guangzhou Institutes of Biomedicine and Health510530GuangzhouChina Bioisland Laboratory 510005GuangzhouChina

Numerous efforts have been attempted to regenerate T cells in culture dish from pluripotent stem cells(PSCs).However,in vitro generated T cells exhibited extremely low activity and compromised immunocompetency in vivo.Here,we describe a two-step protocol for regenerating functional T cells using an inducible Runx1-Hoxa9-PSC(iR9-PSCs)line.The procedure mainly includes generation of induced hematopoietic progenitor cells(iHPCs)in vitro,transplantation,and development of functional induced T cells(iT)in vivo via transplantation.The entire induction process in vitro requires 21 days before iHPCs transplantation.The development of mature T cells in vivo takes 4 to 6 weeks post-transplantation.We provide a simple and reproducible approach for functional T cell regeneration from iR9-PSCs for research purpose.

关键词： Induced hematopoietic progenitor cells pluripotent stem cells T cells regeneration Transplantation

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Regeneration of immunocompetent B lymphopoiesis from pluripotent stem cells guided by transcription factors

引用

Cellular & Molecular Immunology 2022年第4期19卷 492-503页

作者： Qi Zhang Bingyan Wu Qitong Weng Fangxiao Hu Yunqing Lin Chengxiang Xia Huan Peng Yao Wang Xiaofei Liu Lijuan Liu Jiapin Xiong Yang Geng Yalan Zhao Mengyun Zhang Juan Du Jinyong Wang CAS Key Laboratory of Regenerative Biology Guangzhou Institutes of Biomedicine and HealthChinese Academy of SciencesGuangzhou510530China Bioland Laboratory(Guangzhou Regenerative Medicine and Health Guangdong Laboratory) Guangzhou510005China Guangdong Provincial Key Laboratory of stem Cell and Regenerative Medicine Guangzhou Institutes of Biomedicine and HealthChinese Academy of SciencesGuangzhou510530China University of Chinese Academy of Sciences Beijing100049China Institute for stem Cell and Regeneration Chinese Academy of SciencesBeijing100101China GMU-GIBH Joint School of Life Sciences Guangzhou Medical UniversityGuangzhou511436China

Regeneration of functional B lymphopoiesis from pluripotent stem cells(PSCs)is challenging,and reliable methods have not been developed.Here,we unveiled the guiding role of three essential factors,Lhx2,Hoxa9,and Runx1,the simultaneous expression of which preferentially drives B lineage fate commitment and in vivo B lymphopoiesis using PSCs as a cell source.In the presence of Lhx2,Hoxa9,and Runx1 expression,PSC-derived induced hematopoietic progenitors(iHPCs)immediately gave rise to pro/pre-B cells in recipient bone marrow,which were able to further differentiate into entire B cell lineages,including innate B-1a,B-1b,and marginal zone B cells,as well as adaptive follicular B cells.In particular,the regenerative B cells produced adaptive humoral immune responses,sustained antigen-specific antibody production,and formed immune memory in response to antigen challenges.The regenerative B cells showed natural B cell development patterns of immunoglobulin chain switching and hypermutation via cross-talk with host T follicular helper cells,which eventually formed T cell-dependent humoral responses.This study exhibits de novo evidence that B lymphopoiesis can be regenerated from PSCs via an HSC-independent approach,which provides insights into treating B cell-related deficiencies using PSCs as an unlimited cell resource.

关键词： Lhx2 Hoxa9 Runx1 B lymphopoiesis pluripotent stem cells

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Telomere regulation in pluripotent stem cells

引用

Protein & Cell 2014年第3期5卷 194-202页

作者： Yan Huang Puping Liang Dan Liu Junjiu Huang Zhou Songyang Key Laboratory of Reproductive Medicine of Guangdong Province the First Affiliated Hospital and Key Laboratory of Gene_ Engineering of the Ministry of Education School of Life Sciences Sun Yat-sen University Guangzhou 510275 China Cell-Based Assay Screening Core Baylor College of Medicine One Baylor Plaza Houston TX 77030 USA Dan L. Duncan Cancer Center Baylor'College of Medicine one Baylor'Plaza Houston TX 77030 uSA Verna and Marrs Mclean Department of Biochemistry and Molecular Biology Baylor College of Medicine One Baylor Plaza Houston TX 77030 USA

pluripotent stem cells （PSCs） have the potential to pro- duce any types of cells from all three basic germ layers and the capacity to self-renew and proliferate indefinitely in vitro. The two main types of PSCs, embryonic stem cells （ESCs） and induced pluripotent stem cells （iPSCs）, share common features such as colony morphology, high expression of Oct4 and Nanog, and strong alkaline phosphatase activity. In recent years, increasing evi- dences suggest that telomere length represents another important internal factor in maintaining stem cell pluri- potency. Telomere length homeostasis and its structural integrity help to protect chromosome ends from recom- bination, end fusion, and DNA damage responses, ensuring the divisional ability of mammalian cells. PSCs generally exhibit high telomerase activity to maintain their extremely long and stable telomeres, and emerging data indicate the alternative lengthening of telomeres （ALT） pathway may play an important role in telomere functions too. Such characteristics are likely key to their abilities to differentiate into diverse cell types in v/vo. In this review, we will focus on the function and regulation of telomeres in ESCs and iPSCs, thereby shedding light on the importance of telomere length to pluripotency and the mechanisms that regulate telomeres in PSCs.

关键词： telomere pluripotent stem cells alternativelengthening of telomeres （ALT） regulators

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Modeling axonal defects in hereditary spastic paraplegia with human pluripotent stem cells

引用

Frontiers in Biology 2016年第5期11卷 339-354页

作者： Kyle R. Denton Chongchong Xu Harsh Shah Xue-Jun Li Department of Neuroscience University of Connecticut Health Center Farmington CT 06030 USA Department of Biomedical Sciences University of Illinois College of Medicine at Rockford IL 61107 USA MD program College of Medicine at Rockford IL 61107 USA MD program College of Medicine at Rockford IL 61107 USA Department of Btoengmeertng University of Illinois at Chicago IL 60607 USA

BACKGROUND： Cortical motor neurons, also known as upper motor neurons, are large projection neurons whose axons convey signals to lower motor neurons to control the muscle movements. Degeneration of cortical motor neuron axons is implicated in several debilitating disorders including hereditary spastic paraplegia （HSP）. Since the discovery of the first HSP gene, SPASTthat encodes spastin, over 70 distinct genetic loci associated with HSP have been identified. How the mutations of these functionally diverse genes result in axonal degeneration and why certain axons are affected in HSP remain largely unknown. The development of induced pluripotent stem cell （iPSC） technology has provided researchers an excellent resource to generate patient-specific human neurons to model human neuropathological processes including axonal defects. METHODS： In this article, we will first review the pathology and pathways affected in the common forms of liSP subtypes by searching the PubMed database. We will then summarize the findings and insights gained from studies using iPSC-based models, and discuss challenges and future directions. RESULTS： HSPs, a heterogeneous group of genetic neurodegenerative disorders, exhibit similar pathological changes that result from retrograde axonal degeneration of cortical motor neurons. Recently, iPSCs have been generated from several common forms of HSP including SPG4, SPG3A, and SPG11 patients. Neurons derived from HSP iPSCs exhibit impaired neurite outgrowth, increased axonal swellings, and reduced axonal transport, recapitulating disease-specific axonal defects. CONCLUSIONS： These patient-derived neurons offer a unique tool to study the pathogenic mechanisms and explore the treatments for rescuing axonal defects in HSP, as well as other diseases involving axonopathy.

关键词： HSP axonal degeneration pluripotent stem cells spastin atlastin-1

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：