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Evaluation of molecular and immunological methods for diagnosis of tuberculosis pleurisy

Asian Pacific Journal of Tropical Medicine 2008年第4期1卷 14-19页

作者： Maysaa El Sayed Zaki Clinical Pathology Faculty of MedicineMansoura UniversityEgypt

Objective:The inefficiency of conventional laboratory methods for diagnosis of Pleural tuberculosis(TBP) and the reliance on pleural biopsy have motivated the evaluation of alternative diagnostic *** goal was to evaluate different laboratory techniques Ziehl Neelsen,Mantoux skin test,determination of interferon gamma in serum and pleural fluid,polymerase chain reaction and serological study of specific IgG,IgM and IgA beside bacteriological culture by BACTEC 460 TB for rapid and accurate diagnosis of tuberculosis pleurisy. Methods:Patients presented with pleural effusions were subjected to study by ZN,PCR,serological study By specific IgG,IgM and IgA for A60 of tuberculosis compared to culture by BACTEC460 *** gamma was determined both in serum and pleural ***:Mantoux skin test was positive in 19 TBP patients and four patients with exudative pleural effusion.Z.N staining results of pleural biopsy specimens were positive in only 1 of 23 patients(4.3%) in the tuberculous pleural effusion *** was positive in 20 cases of group 1(87%).Serum and pleural fluid interferon had significantly elevated levels(P<0.000 1) in TBP and both measurements had significant correlation in TBP(P<0.000 1).The serum IgA ELISA test was positive in 7/23(30.4%),IgM was positive in 17/23(73.9%) patients and IgG was positive in 16/23(69%) *** of the non TBP had either ZN,PCR or positive serum IgA,IgG,or *** the positive results for IgG and IgM were combined together the serological tests correcdy identified 20/23(87%) of patients. Conclusion:We suggest that in TBP serological diagnosis by combined use of IgG and IgM for A60 antigen with serum determination of interferon gamma can provide rapid and non invasive diagnostic tool that can justify the starting of chemotherapy while awaiting the results of culture.

关键词： Tuberculous pleurisy Molecular diagnosis Tuberculin Serology Interferon gamma

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pleurisy in Hemodialysis Patients: Epidemiological and Etiological Aspects at Donka National Hemodialysis Center

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Open Journal of Nephrology 2021年第1期11卷 1-8页

作者： M. Traoré F. Diakité M. S. Baldé I. Chérif M. Sidibé A. B. Bah N. M. A. Nyaméni M. L. T. Camara M. Camara M. K. Kanu A. O. Bah M. L. Kaba Department of Nephrology-Hemodialysis Conakry Guinea Medical Care Clinic Freetown Sierra-Leone

Introduction: Pleural effusion being a frequent complication in hemodialysis patients, its etiologies are diverse and the diagnosis is easy, based on clinical and radiological proofs. The main objective of this study was to describe the epidemiological and etiological profiles of pleurisy in hemodialysis patients at the National Hemodialysis Center of Donka National Hospital. Patients and methods: it was a retrospective, descriptive and analytical study that extended over a period of three years from January 1, 2017 to December 31, 2019. It concerned, among hemodialysis patients in the center during the study period, all those who had a confirmed pleurisy on chest x-ray. The variables were epidemiological (based on frequency, sex and age), clinical (based on history of the disease, physical examination looking for a reduction or elimination of vesicular murmur) and paraclinical (mainly radiological). Results: Among 286 patients undergoing hemodialysis in our center, pleural effusion was diagnosed in 35 or 12.24%. The average age of our patients was 52.22 years with ranges of 18 and 78 years. The sex ratio M/F was 2.5. Bilateral pleurisy was found in 51.43% of patients;unilateral right in 40% of cases and unilateral left in 8.57%. We observed 68.57% citrus yellow fluid and 31.43% sero haematic fluid. The bacteriology of the pleural fluid was positive in 62.86% against 37.14% negative. Tumor, tuberculosis and non-specific bacterial etiologies have been encountered. Conclusion: pleurisy is therefore a frequent complication in hemodialysis patients at Donka National Hemodialysis Center. Etiological research is a major step for better management of these patients. Improving the technical platform of the laboratories should be an important contribution to this stage.

关键词： pleurisy Hemodialysis Epidemiology Etiologies

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Genetic Association of Interferon Gamma Induced Protein-10 (IP-10), <i>CXCL-</i>10 Gene Polymorphisms with TB pleurisy Susceptibility in South Indian Population

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Open Journal of Immunology 2015年第2期5卷 72-78页

作者： Ghousunnissa Sheikh Venkata Sanjeev Kumar Neela Satya Sudheer Pydi Naveen Chandra Suryadevara Ramulu Gaddam Suman Latha Gaddam Sai Kumar Auzumeedi Vijaya Lakshmi Valluri LEPRA-India Blue Peter Public Health & Research Centre Hyderabad India Government Chest & General Hospital Hyderabad India Bhagwan Mahavir Medical Research Centre Hyderabad India Osmania University Hyderabad India

CXCL-10 known as Interferon gamma-induced protein 10 (IP-10) or small-inducible cytokine 10 is a 8.7 kDa protein, which is secreted in response to IFN-γ by monocytes, endothelial cells and fi-broblasts. It has chemo-attraction for monocytes/macrophages, T cells, NK cells and dendritic cells in promotion of T cell adhesion to endothelial cells. In the present study, we investigated whether polymorphisms in CXCL-10 gene have any role in the manifestation of Tuberculous (TB) pleurisy. Two SNPs in CXCL-10 promoter region (﹣1447A > G and ﹣135G > A) were genotyped in patients with TB pleurisy (n = 186), Pulmonary TB patients (n = 159) and healthy controls (n = 205) by PCR-RFLP. Disease associations were statistically analyzed by Fisher exact test. At the ﹣135G > A position, the frequencies of genotype GA and allele G were significantly high in TB pleurisy patients compared to healthy controls. While the frequencies of genotype AA and allele A were significantly low in TB pleurisy patients compared to healthy controls. The frequency of haplotype A-G with the combination of 1447A > G and ﹣135G > A was significantly high in TB pleurisy. Our results reveal that genotype GA and allele G at ﹣135G > A position were strongly associated with susceptibility to tuberculous pleurisy. The GA genotype may be a useful genetic marker for early detection of the disease in high risk individuals.

关键词： CXCL-10 Polymorphism Haplotype Susceptibility Tuberculous pleurisy

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Research Progress of microRNA in the Diagnosis of Tuberculous pleurisy

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Journal of Biosciences and Medicines 2024年第7期12卷 160-169页

作者： Surui Chen Xinyu Li Jiale Chen Yunnan Shen Boyu Hui Wei Wu Clinical Medicine School Xian Medical University Xian China Department of Respiratory and Critical Care Medicine The Second Affiliated Hospital of Xian Medical University Xian China

Tuberculosis has become a major public health and social problem threatening human health, and a large proportion of pulmonary tuberculosis patients are associated with tuberculous pleurisy (TP). Therefore, it is of great significance to find markers with high specificity and sensitivity for the rapid and accurate diagnosis and differential diagnosis of TP under the severe background of high infectivity and mortality due to the occult nature of TP. The extraction of microRNA (miRNA) from pleural effusion satisfies the characteristics of strong operability. miRNA exists not only in cells, but also in various body fluids and participates in the pathophysiological process of various diseases including infectious diseases. miRNA is a highly specific biomarker in pleural fluid in patients with TP. Therefore, this article provides a review of the research progress of mRNA in tuberculous pleurisy.

关键词： Tuberculous pleurisy miRNA Diagnosis

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A single administration of fish oil inhibits the acute inflammatory response in rats

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Asian Pacific Journal of Tropical Medicine 2017年第8期10卷 830-837页

作者： Laura Licia Milani de Arruda Franciele Queiroz Ames Damila Rodrigues de Morais Renata Grespan Ana Paula Maziero Gil Maria Angelica Raffaini Covas Pereira Silva Jesui Vergilio Visentainer Roberto Kenji Nakamura Cuman Ciomar Aparecida Bersani-Amado Department of Pharmacology and Therapeutics State University of MaringaMaringa-PRBrazil Department of Chemistry State University of MaringaMaringa-PRBrazil

Objective: To investigate the antiinflammatory effects of a single administration of fish oil(FO) on the acute inflammatory response. Methods: The paw edema and pleurisy models were used to evaluate the effects of FO dissolved in olive oil(FOP) orally administered in a single dose in rats. Nitric oxide(NO) concentrations in the pleural exudate were performed according to the Griess method and the cytokine concentrations were determined by Luminex bead-based multiplex assay. Results: FOP treatment(30 and 300 mg/kg) significantly reduced paw edema. FOP treatment at 18.75, 37.5, 75.0, 150.0, and 300 mg/kg decreased both the volume of pleural exudate and cellular migration into the pleural cavity and each of these doses presented the same effectiveness. Treatment with FOP(300 mg/kg) reduced NO, TNF-α, IL-1β, and IL-6 concentrations in the pleural exudate. Conclusions: The present data provide evidence that FO has inhibitory effects on the acute inflammatory response when administered in a single dose in rats. This effect might be attributable to a direct inhibitory effect of FO on the production or release of inflammatory mediators that are involved in the pathological processes evaluated herein.

关键词： Omega-3 fatty acid Polyunsaturated fatty acid pleurisy Leukocyte migration EPA DHA

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胸腔积液腺苷脱氨酶测定对结核性胸膜炎的诊断价值

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医学检验与临床 2006年第2期18卷 44-,47页

作者：华裕忠许俊华陈敏江国峰青岛市胸科医院青岛266043

目的探讨胸腔积液腺昔脱氨酶(ADA)活性检测对结核性胸膜炎的诊断价值。方法对96例结核性胸腔积液和32例恶性胸腔积液标本进行ADA检测,同时与本人血清ADA活性作比较。结果结核性胸腔积液ADA活性明显增高(48.8±12.8),恶性胸腔积液ADA活... 详细信息

目的探讨胸腔积液腺昔脱氨酶(ADA)活性检测对结核性胸膜炎的诊断价值。方法对96例结核性胸腔积液和32例恶性胸腔积液标本进行ADA检测,同时与本人血清ADA活性作比较。结果结核性胸腔积液ADA活性明显增高(48.8±12.8),恶性胸腔积液ADA活性无异常变化(9.23±1.6),两者相比差别有极显著意义(P0.05)。结核组ADA胸水/血清>1(3.96±2.15),非结核组胸水/血清<1(0.78±0.14),两者相比差别有极显著意义(p<0.01)。结论胸腔积液腺苷脱氨酶测定对结核性胸膜炎具有很高的临床诊断价值。

关键词：结核性胸腔积液腺苷脱氨酶酶测定结核性胸膜炎诊断价值 tuberculous pleurisy diagnosis of 恶性胸腔积液活性检测人血清异常变化胸水比差非结核临床方法标本

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