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A near-infrared plasma membrane-specific AIE probe for fluorescence lifetime imaging of phagocytosis

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Science China Chemistry 2022年第5期65卷 979-988页

作者： Ming-Yu Wu Jong-Kai Leung Chuen Kam Tsu Yu Chou Jia-Li Wang Xueqian Zhao Shun Feng Sijie Chen Ming Wai Lau Centre for Reparative Medicine Karolinska InstitutetHong KongChina Sichuan Engineering Research Center for Biomimetic Synthesis of Natural Drugs School of Life Science and EngineeringSouthwest Jiaotong UniversityChengdu 610031China

Phagocytosis is a biological process that plays a key role in host defense and tissue *** approaches for real-time imaging of phagocytosis are highly desired but ***,an AIE-active near-infrared fluorescent probe,named TBTCP,was developed for fluorescence lifetime imaging of *** could selectively label the cell plasma membrane with fast staining,wash-free process,high signal-to-background ratio,and excellent *** membrane statuses under different osmolarities as well as macrophage phagocytosis of bacteria or large silica particles in early stages could be reported by the fluorescence lifetime changes of *** with current fluorescence imaging methods,which target the bioenvironmental changes in the late phagocytosis stage,this approach detects the changes in the cell membrane,thus giving a faster response to *** article provides a functional tool to report the phagocytic dynamics of macrophages which may greatly contribute to the studies of phagocytic function-related diseases.

关键词： fluorescence lifetime imaging phagocytosis plasma membrane aggregation-induced emission near-infrared

Changes of plasma membrane ATPase activity, membrane potential and transmembrane proton gradient in Kandelia candel and Avicennia marina seedlings with various salinities

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Journal of Environmental Sciences 2004年第5期16卷 742-745页

作者： ZHAOZhong-qiu ZHENGHai-lei ZHUYong-guan ResearchCenterforEco-EnvironmentalSciences ChineseAcademyofSciencesBeijing100085China SchoolofLifeSciences XiamenUniversityXiamen361005China

The salt-secreting mangrove, Avicennia marina, and non-salt-secreting mangrove, Kandelia candel were cultivated in sand with various salinities(0‰, 10‰, 20‰, 30‰, 40‰) for 60 d. plasma membrane vesicles of high-purity in leaves and roots of *** and K. candel seedlings were obtained by two-phase partitioning. The function of the plasma membranes, the activity of ATPase, membrane potential and transmembrane proton gradient, at various salinities were investigated. The results showed that within a certain range of salinity(A. marina and roots of K. candel: 0—30‰; leaves of ***: 0—20‰), the activity of ATPase increased with increasing salinity, while high salinity(above 30‰ or 20‰) inhibited ATPase activity. In comparison with A. marina, K. candel appeared to be more sensitive to salinity. The dynamics of membrane potential and transmembrane proton gradient in leaves and roots of A. marina and K. candel seedlings were similar to that of ATPase. When treated directly by NaCl all the indexes were inhibited markedly: there was a little increase within 0—10‰(K. candel) or 0—20‰(A. marina) followed by sharp declining. It indicated that the structure and function of plasma membrane was damaged severely.

关键词： salinity A. marina K. candel plasma membrane H-ATPase Ca-ATPase membrane potential transmembrane proton gradient

Effects of salinity on activities of H^+-ATPase, H^+-PPase and membrane lipid composition in plasma membrane and tonoplast vesicles isolated from soybean(Glycine max L.) seedlings

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Journal of Environmental Sciences 2005年第2期17卷 259-262页

作者： YUBing-jun LAMHon-ming SHAOGui-hua LIUYou-ling CollegeofLifeSciences NanjingAgriculturalUniversityNanjing210095China／／DepartmentofBiologyTheChineseUniversityofHongKongShatinN.T.HongKongChina DepartmentofBiology TheChineseUniversityofHongKongShatinN.T.HongKongChina DepartmentofBiology TheChineseUniversityofHongKongShatinN.T.HongKongChina／／InstituteofCropBreedingandCultivationChineseAcademyofAgriculturalSciencesBeijing100081China CollegeofLifeSciences NanjingAgriculturalUniversityNanjing210095China

The effects of NaCl stress on the H +-ATPase, H +-PPase activity and lipid composition of plasma membrane(PM) and tonoplast(TP) vesicles isolated from roots and leaves of two soybean cultivars(Glycine max L.) differing in salt tolerance(Wenfeng7, salt-tolerant; Union, salt-sensitive) were investigated. When Wenfeng7 was treated with 0.3%(W/V) NaCl for 3 d, the H +-ATPase activities in PM and TP from roots and leaves exhibited a reduction and an enhancement, respectively. The H +-PPase activity in TP from roots also increased. Similar effects were not observed in roots of Union. In addition, the increases of phospholipid content and ratios of phospholipid to galactolipid in PM and TP from roots and leaves of Wenfeng7 may also change membrane permeability and hence affect salt tolerance.

关键词： salinity Glycine max L. plasma membrane tonoplast H +-ATPase H +-PPase membrane lipid composition

Functional analysis of MdSUT2.1,a plasma membrane sucrose transporter from apple

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Journal of Integrative Agriculture 2023年第3期22卷 762-775页

作者： ZHANG Bo FAN Wen-min ZHU Zhen-zhen WANG Ying ZHAO Zheng-yang State Key Laboratory of Crop Stress Biology for Arid Areas College of HorticultureNorthwest A&F UniversityYangling 712100P.R.China Shaanxi Research Center of Apple Engineering and Technology Yangling 712100P.R.China

Sugar content is a determinant of apple(Malus×domestica Borkh.)***,the molecular mechanism underlying sucrose accumulation in apple fruit remains ***,this study reported the role of the sucrose transporter MdSUT2.1 in the regulation of sucrose accumulation in *** MdSUT2.1 gene encoded a protein with 612 amino acid residues that could be localized at the plasma membrane when expressed in tobacco leaf ***2.1 was highly expressed in fruit and was positively correlated with sucrose accumulation during apple fruit ***,complementary growth assays in a yeast mutant validated the sucrose transport activity of ***2.1 overexpression in apples and tomatoes resulted in significant increases in sucrose,fructose,and glucose contents compared to the wild type(WT).Further analysis revealed that the expression levels of sugar metabolism-and transport-related genes SUSYs,NINVs,FRKs,HXKs,and TSTs increased in apples and tomatoes with MdSUT2.1 overexpression compared to ***,unlike the tonoplast sugar transporters MdTST1 and MdTST2,the promoter of MdSUT2.1 was not induced by exogenous *** findings provide valuable insights into the molecular mechanism underlying sugar accumulation in apples.

关键词： apple MdSUT2.1 sugar transport plasma membrane

Proteomic Analysis of Rice plasma membrane-associated Proteins in Response to Chitooligosaccharide Elicitors

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Journal of Integrative Plant Biology 2007年第6期49卷 863-870页

作者： Fang Chen Qun Li Zuhua He State Key Laboratory of Plant Molecular Genetics Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences the Chinese Academy of Sciences Shanghai 200032 China

Chitooligomers or chitooligosaccharides （COS） are elicitors that bind to the plasma membrane （PM） and elicit various defense responses. However, the PM-bound proteins involved in elicitor-mediated plant defense responses still remain widely unknown. In order to get more information about PM proteins involved in rice defense responses, we conducted PM proteomic analysis of the rice suspension cells elicited by COS. A total of 14 up- or downregulated protein spots were observed on 2-D gels of PM fractions at 12 h and 24 h after COS incubation. Of them, eight protein spots were successfully identified by MS （mass spectrography） and predicted to be associated to the PM and function in plant defense, including a putative PKN/PRK1 protein kinase, a putative pyruvate kinase isozyme G, a putative zinc finger protein, a putative MAR-binding protein MFP1, and a putative calcium-dependent protein kinase. Interestingly, a COS-induced pM5-like protein was identified for the first time in plants, which is a transmembrane nodal modulator in transforming growth factor-β（TGFβ） signaling in vertebrates. We also identified two members of a rice polyprotein family, which were up-regulated by COS. Our study would provide a starting point for functionality of PM proteins in the rice basal defense.

关键词： defense response plasma membrane proteomics rice chitooligosaccharide.

Vacuolar Sorting Receptor （VSR） Proteins Reach the plasma membrane in Germinating Pollen Tubes

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Molecular Plant 2011年第5期4卷 845-853页

作者： Hao Wang Xiao-Hong Zhuang Stefan Hillmer David G. Robinson Li-Wen Jiang School of Life Sciences Centre for Cell and Developmental Biology The Chinese University of Hong Kong Shatin New Territories Hong Kong China State Key Laboratory of Agrobiotechnology The Chinese University of Hong Kong Shatin New Territories Hong Kong China Department of Cell Biology Heidelberg Institute for Plant Science University of Heidelberg Germany

Vacuolar sorting receptors （VSRs） are type I integral membrane proteins that mediate the vacuolar transport of soluble cargo proteins via prevacuolar compartments （PVCs） in plants. Confocal immunofluorescent and immunogold Electron Microscope （EM） studies have localized VSRs to PVCs or multivesicular bodies （MVBs） and trans-Golgi network （TGN） in various plant cell types, including suspension culture cells, root cells, developing and germinating seeds. Here, we provide evidence that VSRs reach plasma membrane （PM） in growing pollen tubes. Both immunofluorescent and immunogold EM studies with specific VSR antibodies show that, in addition to the previously demonstrated PVC/MVB localization, VSRs also localize to PM in lily and tobacco pollen tubes prepared from chemical fixation or high-pressure freezing/frozen substitution. Such a PM localization suggests an additional role of VSR proteins in mediating protein transport to PM and endocytosis in growing pollen tubes. Using a high-speed Spinning Disc Confocal Microscope, the possible fusion between VSR-positive PVC organelles and the PM was also observed in living tobacco pollen tubes transiently expressing the PVC reporter GFP-VSR. In contrast, the lack of a prominent PM localization of GFP-VSR in living pollen tubes may be due to the highly dynamic situation of vesicular transport in this fast-growing cell type.

关键词： plasma membrane pollen tube vacuolar sorting receptor.

A critical review on natural compounds interacting with the plant plasma membrane H^(+)-ATPase and their potential as biologicals in agriculture

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Journal of Integrative Plant Biology 2022年第2期64卷 268-286页

作者： Nanna W.Havshφi Anja T.Fuglsang Department of Plant and Environmental Sciences Section for Transport BiologyUniversity of CopenhagenFrederiksbergCopenhagenDK-1871 Denmark

The plant plasma membrane(PM)H^(+)-ATPase is an essential enzyme controlling plant growth and *** is an important factor in response to abiotic and biotic stresses and is subject to tight *** are in demand for new sustainable natural growth regulators and as a key enzyme for regulation of transport into the plant cell the PM H^(+)-ATPase is a potential target for *** this review,we have evaluated the known non-protein natural compounds with regulatory effects on the PM H^(+)-ATPase,focusing on their mechanism of action and their potential as biologicals/growth regulators in plant production of future sustainable agriculture.

关键词： biologicals cyclic-lipodepsipeptides H^(+)-ATPase H+pump natural compounds phosphatidylinositol plasma membrane pore forming compound tenuazonic acid

The connection of cytoskeletal network with plasma membrane and the cell wall

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Journal of Integrative Plant Biology 2015年第4期57卷 330-340页

作者： Zengyu Liu Staffan Persson Yi Zhang Max-Planck Institute for Molecular Plant Physiology ARC Centre of Excellence in Plant Cell Walls School of BotanyUniversity of Melbourne

The cell wall provides external support of the plant cells, while the cytoskeletons including the microtubules and the actin filaments constitute an internal framework. The cytoskeletons contribute to the cell wall biosynthesis by spatially and temporarily regulating the transportation and deposition of cell wall components. This tight control is achieved by the dynamic behavior of the cytoskeletons, but also through the tethering of these structures to the plasma membrane. This tethering may also extend beyond the plasma membrane and impact on the cell wall, possibly in the form of a feedback loop. In this review, we discuss the linking components between the cytoskeletons and the plasma membrane, and/or the cell wall. We also discuss the prospective roles of these components in cell wall biosyn- thesis and modifications, and aim to provide a platform for further studies in this field.

关键词： Cytoskeleton plant cell wall plasma membrane

Actin Dynamics Regulates Voltage-Dependent Calcium-Permeable Channels of the Vicia faba Guard Cell plasma membrane

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Journal of Integrative Plant Biology 2009年第10期51卷 912-921页

作者： Wei Zhang Liu-Min Fan National Laboratory of Plant Physiology and Biochemistry College of Biological Sciences China Agricultural University Beijing 100094 China School of Life Sciences Shandong University Jinan 250100 China peking-Yale Joint Center for Plant Molecular Genetics and Agro-Biotechnology National Laboratory of Protein Engineering and Plant Genetic Engineering College of Life Sciences Peking University Beijing 100871 China

Free cytosolic Ca^2＋（[Ca^2＋]cyt） is an ubiquitous second messenger in plant cell signaling, and [Ca^2＋]cyt elevation is associated with Ca^2＋-permeable channels in the plasma membrane and endomembranes regulated by a wide range of stimuli. However, knowledge regarding Ca^2＋ channels and their regulation remains limited in planta. A type of voltage- dependent Ca^2＋-permeable channel was identified and characterized for the Vicia faba L. guard cell plasma membrane by using patch-clamp techniques. These channels are permeable to both Ba^2＋ and Ca^2＋, and their activities can be inhibited by micromolar Gd^3＋. The unitary conductance and the reversal potential of the channels depend on the Ca^2＋ or Ba^2＋ gradients across the plasma membrane. The inward whole-cell Ca^2＋（Ba^2＋） current, as well as the unitary current amplitude and NPo of the single Ca^2＋ channel, increase along with the membrane hyperpolarization. Pharmacological experiments suggest that actin dynamics may serve as an upstream regulator of this type of calcium channel of the guard cell plasma membrane. Cytochalasin D, an actin polymerization blocker, activated the NPo of these channels at the single channel level and increased the current amplitude at the whole-cell level. But these channel activations and current increments could be restrained by pretreatment with an F-actin stabilizer, phalloidin. The potential physiological significance of this regulatory mechanism is also discussed.

关键词： actin dynamics calcium-permeable channels guard cell patch clamp plasma membrane Vicia faba.