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plant extract-loaded bacterial cellulose composite membrane for potential biomedical applications

Journal of Bioresources and Bioproducts 2021年第1期6卷 26-32页

作者： Atiya Fatima Sumayia Yasir MohdShariq Khan Sehrish Manan Muhammad Wajid Ullah Mazhar Ul-Islam Department of Chemical Engineering College of EngineeringDhofar UniversitySalalahSultanate of Oman Department of Biomedical Engineering Huazhong University of Science and TechnologyWuhan 430074China

Bacterial cellulose(BC)has been extensively explored as biomaterial for various biomedical applications owing to its non-toxic nature and unique structural morphology and impressive physicochemical and mechanical ***,its high production cost and lack of antimicrobial activity have restricted its large-scale production and therapeutic ***,the current study is aimed to devise a strategy for low-cost BC production and develop its composite with bioactive materials to bless it with antimicrobial ***,5 mm thick reticulated fibrous and highly porous BC was produced by utilizing the wasted rotten tomatoes as the production *** produced bacterial cellulose waste(BCW)(i.e.,produced from wastes)was ex-situ modified with bioactive plant extract(PE)obtained from Euclea schimperi,and the bactericidal activity of the developed BCW/PE was evaluated against Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli through disc diffusion and colony forming unit(CFU)count *** BCW/PE composite showed high bactericidal activities against *** and produced clear inhibition zone whereas negligible activity was observed against ***,indicating its bactericidal activity mainly against the Gram-positive ***,this study illustrates that there is a huge potential for developing valuable biomaterials from food wastes and utilizing their liquid holding capabilities for value-added applications in medical and pharmaceutical fields.

关键词： Bacterial cellulose Food waste plant extract Bactericidal activity Biomedical application

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Anti-Fertility Effects and Mechanism of the plant extract Shikonin on Mice

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Journal of Biosciences and Medicines 2016年第8期4卷 30-39页

作者： Heping Fu Darhan Bao Man Duhu Shuai Yuan An Xing Suwen Yang Xiaodong Wu Inner Mongolia Agricultural University Hohhot China Key Laboratory of Prataculture and Grassland Resources Ministry of Education Hohhot China

Controlling fertility of rodent pests has become an effective means of controlling the population of grassland rodents in China. Recently, research has focused on how to select environmentally-friendly sterilants without pollution effects, and to realize sustainable control of pest rodent populations. Sterilants from plant extracts have been mainly selected. In this study, mice were used as the experimental subjects for research on the anti-fertility effects of plant extracts of shikonin and the anti-fertility mechanism of shikonin extract was determined. The mice were divided into four groups, including one control group and three experimental groups. There were three applications of shikonin extract in different concentrations (5 mg·kg-1, 20 mg·kg-1 and 50 mg·kg-1). The mice gavage experiments indicated that a shikonin concentration of 50 mg·kg-1 had the expected anti-fertility effects. Mice copulation experiments showed that the 50 mg·kg-1 shikonin treatment had significant anti-fertility effects on both female-treatment and female-male-treatment groups. The results of the PCR analysis on the AgRP and ghrelin mRNA from female ovaries and male testicles indicated that shikonin could control mice reproduction by regulating the pituitary gonadal axis. Shikonin, as plant source sterile agent, would have more ideal effects for functioned both sexes sterility.

关键词： Anti-Fertility Mechanism plant extract Shikonin Mice

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On-line two dimensional pre-column based on carbon fiber for MS/MS comprehensive analysis of plant extract

On-line two dimensional pre-column based on carbon fiber for...

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中国化学会第二届全国质谱分析学术报告会

作者： Donghao Li Biao Ma Jinhua Zhao Key Laboratory of Natural Resource of the Changbai Mountain and Functional Molecular （Yanbian University） Ministry of Education

A sample pretreatment technique based on the tandem nano carbon fiber columns has been developed to pre-separating different polar components in the plant extract. The first dimensional separation on the short carbon fibers column, apolar components are retained and analyzed, thereby the medium polar components and polar components are automatically eluted at the dead time from short carbon fibers column to the second dimensional separation on the activated carbon fiber column, where these components are subsequently analyzed [1,2]. The different polar components are determined with MS/MS technique. It obtains more components, higher resolution to compare to directly injecting plant extract to HPLC–MS/MS, largely reduces the amount of organic solvent and labor to compare with traditional sample pretreatment [3-5]. The feasibility and reliability of the method was evaluated by the analysis of a mixture of 13 standard compounds. Under the optimized conditions, recoveries of targets ranged from 75.4% to 93.9%, relative standard deviation（RSD%） ranged below than 14.6%, correlation coefficients were above 0.99 across a certain concentration. The limits of quantitation for these targets in plant sample ranged from 0.10 ng to 11.0 ng. Finally, the method was applied to determine of real plant samples of Changbai Mountaion. The results show that this method is simple, rapid, economic, easy to operate, high throughput, make plant sample total analysis come true and it has widely application prospect in comprehensive analysis of complex plant samples（Fig. 1）.

关键词： Two dimension carbon fibers sample treatment plant extract HPLC-MS/MS

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Efficient reduction of Cr(Ⅵ)by guava(Psidium guajava)leaf extract and its mitigation effect on Cr toxicity in rice seedlings

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Journal of Environmental Sciences 2024年第7期141卷 1-15页

作者： Maodi Ran Yongqing Lu Yanzhen Ren Li He Jiaokun Li School of Minerals Processing and Bioengineering Central South UniversityChangsha 410083China

Hexavalent chromium(Cr(Ⅵ))is a toxic element that has negative impacts on crop growth and *** plant extracts to convert toxic Cr(Ⅵ)into less toxic Cr(Ⅲ)may be a more favorable option compared to chemical reducing *** this study,the potential effects and mechanisms of using an aqueous extract of Psidium guajava ***(AEP)in reducing Cr(Ⅵ)toxicity in rice were comprehensively ***,the reducing power of AEP for Cr(Ⅵ)was confirmed by the cyclic voltammetry combined with X-ray photoelectron spectroscopy(XPS)*** highest Cr(Ⅵ)reduction efficiency reached approximately 78%under 1.5mg gallic acid equivalent(GAE)/mL of AEP and 10 mg/L Cr(Ⅵ)***,Cr(Ⅵ)stress had a significant inhibitory effect on rice ***,the exogenous application of AEP alleviated the growth inhibition and oxidative damage of rice under Cr(Ⅵ)stress by increasing the activity and level of enzymatic and non-enzymatic ***,the addition of AEP restored the ultrastructure of root cells,promoted Cr adsorption onto root cell walls,and limited the translocation Cr to *** shoots,AEP application also triggered the expression of specific genes involved in Cr defense and detoxification response,including photosynthesis pathways,antioxidant systems,flavonoids biosynthesis,and plant hormone signal *** results suggest that AEP is an efficient reduction agent for Cr(Ⅵ),and exogenous application of AEP may be a promising strategy to mitigate the harm of Cr(Ⅵ)on rice,ultimately contributing to improved crop yield in Crcontaminated environments.

关键词： Hexavalent chromium plant extract Reduction Rice plants Mechanisms

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Antioxidant Activity and Phytochemical Screening in Acacia rigidula Benth. Leaves

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Advances in Bioscience and Biotechnology 2024年第1期15卷 15-38页

作者： Aneth Ochoa-Negrete Rene Rangel Marcus Antonius Ynalvez Ruby A. Ynalvez Department of Biology and Chemistry Texas A&M International University Laredo TX USA Department of Social Sciences Texas A&M International University Laredo TX USA

This study aimed to compare the total phenolic content (TPC) and antioxidant activities of A. rigidula extracts. It also aimed to identify phenolic acids present in the extracts. The 2,2-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP), and ferric thiocyanate lipid peroxidation antioxidant assays were performed. High performance liquid chromatography was used to identify phenolic acids. There was no solvent effect on TPC nor on scavenging activities, and inhibition of lipid peroxidation (p > 0.05) among solvent extracts. On the other hand, 1:1:3 water: acetone: methanol extract (10.22 mg GAE/g sample) had significantly higher reducing potential than 50% ethanol extract (EE) (9.259 mg GAE/g sample) (p extract (9.781 mg GAE/g sample) (p > 0.05). Phenolic fraction designated as fraction 4 had the highest antioxidant activity (p extracts contain gallic, caffeic, vanillic, p-coumaric, salicylic acids and vanillin.

关键词： Acacia plant extract Antioxidant Activity Total Phenolic Acid HPLC Gallic Acid

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plant phenolic extracts for the quality protection of frying oil during deep frying:Sources,effects,and mechanisms

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粮油科技:英文版 2023年第3期6卷 148-161页

作者： Fa Wang Yixi Sun Shanshan Li Jing Yan Wen Qin Ahmed S.M.Saleh Qing Zhang College of Food Science Sichuan Agricultural UniversityYa'anSichuanChina Key Laboratory of Agricultural Product Processing and Nutrition and Health of the Ministry of Agriculture and Rural Affairs(Jointly Built by the Ministry and the Province) Ya'anSichuanChina Department of Food Science and Technology Faculty of AgricultureAssiut UniversityEgypt

Protection of frying oil from deterioration by adding plant phenolic extracts to guarantee the quality of fried foods becomes the primary approach to promote the sustainable development of deep ***,sources,antioxidant effects,and mechanisms of plant phenolic extracts recently applied in the quality protection of frying oil as well as challenges for the actual use of these extracts are comprehensively reviewed in this ***,herbs,berries,tea leaves,and fruit and vegetable wastes are common sources for preparing phenolic extracts showing comparative antioxidant capacity referring to the synthetic *** general effect of using these natural antioxidants is the improvement of thermal stability to extend the shelf life of frying oil and thus the modification of edible quality of fried ***,the increases in common quality attributes and amount of hazardous products and the oxidative reduction of unsaturated triacylglycerols without negatively influencing the sensory quality are inhibited when suitable plant extracts are *** incorporation of plant phenolic extracts other than synthetic counterparts in frying oil has been demonstrated as a potential method to improve the frying performance of ***,challenges for the scale application of plant phenolic extracts,such as the purity,thermal stability,and antioxidant timing,are still needed to be further investigated.

关键词： Frying oil Phenolic antioxidants plant extract Physicochemical properties Antioxidant mechanisms

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Nutritional strategies to alleviate oxidative stress in sows

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Animal Nutrition 2022年第2期9卷 60-73页

作者： Qihui Li Siwang Yang Fang Chen Wutai Guan Shihai Zhang Guangdong Province Key Laboratory of Animal Nutrition Control College of Animal ScienceSouth China Agricultural UniversityGuangzhou 510642China College of Animal Science and National Engineering Research Center for Breeding Swine Industry South China Agricultural UniversityGuangzhou 510642China Guangdong Laboratory for Lingnan Modern Agriculture South China Agricultural UniversityGuangzhou 510642China

The performance of high-yielding sows is directly related to the productivity of pig farming. Fetaldevelopment mainly occurs during the last month of pregnancy, and the aggressive metabolic burden ofsows during this stage eventually leads to systemic oxidative stress. When affected by oxidative stress,sows exhibit adverse symptoms such as reduced feed intake, hindered fetal development, and evenabortion. In addition, milk synthesis during the lactation period causes a severe metabolic burden. Thebiological response to oxidative stress during this period is associated with a decrease in milk production,which further affects the growth of piglets. Understanding the nutritional strategies to alleviateoxidative stress in sows is crucial to maintain their reproduction and lactation performance. Recently,advances have been made in the field of nutrition to relieve oxidative stress in sows during late pregnancyand lactation. This review highlights the nutritional strategies to relieve oxidative stress in sowsreported within the last 20 years.

关键词： Sow Oxidative stress Nutritional strategy plant extract Selenium Vitamin E

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Effect of dietary magnolia bark extract supplementation in finishing pigs on the oxidative stability of meat

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Journal of Animal Science and Biotechnology 2023年第1期14卷 200-211页

作者： Ruggero Menci Hajer Khelil‑Arfa Alexandra Blanchard Luisa Biondi Marco Bella Alessandro Priolo Giuseppe Luciano Antonio Natalello Dipartimento Di Agricoltura Alimentazione e Ambiente(Di3A)University of CataniaVia Valdisavoia 595123 CataniaItaly. Pancosma ADM A One Business Center1180 RolleSwitzerland.

Background:Magnolia bark extract(MBE)is a natural supplement with antioxidant,anti-inflammatory,and antimicrobial *** properties suggest that the dietary supplementation in livestock could improve the quality of ***,the aim of this study was to investigate,for the first time,the effect of dietary MBE supplementation(0.33 mg/kg)in finishing pigs on the oxidative stability of *** stability is of paramount importance for pork,as it affects storage,retail,and consumer *** the purpose,the fatty acid profile,cholesterol,fatsoluble vitamins,antioxidant enzymes(catalase,glutathione peroxidase,and superoxide dismutase),non-enzymatic antioxidant capacity(TEAC,FRAP,and Folin-Ciocalteu assays),color stability,and lipid stability of pork were ***:Concerning carcass characteristics,dietary MBE did not affect cold carcass yield,but reduced(P=0.040)the chilling weight *** meat from pigs fed MBE had a lower(P=0.031)lightness index than the control *** effect on intramuscular fat,cholesterol,and fatty acid profile was *** MBE did not affect the content of vitamin E(α-tocopherol andγ-tocopherol)in pork,whereas it reduced(P=0.021)the retinol *** catalase activity was 18%higher(P=0.008)in the meat from pigs fed MBE compared with the control *** MBE supplementation reduced(P=0.039)by 30%the thiobarbituric acid reactive substances(TBARS)in raw pork over 6 d of aerobic refrigerated ***,no effect on lipid oxidation was observed in cooked ***,the meat from pigs fed MBE reduced Fe3+-ascorbate catalyzed lipid oxidation in muscle homogenates,with a lower(P=0.034)TBARS value than the control group after 60 min of ***:Dietary MBE supplementation in finishing pigs delayed the lipid oxidation in raw *** effect was combined with an increased catalase *** results suggest that dietary MBE could have implications for improving the shelf-life of pork

关键词： Antioxidant capacity Antioxidant enzyme Color Fatty acid Lipid oxidation plant extract Pork Shelf-life TBARS Vitamin

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Potential of a mixture of eugenol and garlic tincture to improve performance and intestinal health in broilers under necrotic enteritis challenge

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Animal Nutrition 2022年第1期8卷 26-37页

作者： Alip Kumar Nishchal K.Sharma Sarbast K.Kheravii Chake Keerqin Catherine Ionescu Alexandra Blanchard Shu-Biao Wu School of Environmental and Rural Science University of New EnglandArmidaleNSW2351Australia ADMi Pancosma SA A-One Business CenterLa piece 3CH-1180RolleSwitzerland

plant extracts(PE)are gaining increased attention as potential alternatives to in-feed antimicrobials(AM)due to their known antimicrobial *** study was conducted to examine the potential of PE,a microencapsulated product composed of eugenol and garlic tincture as an alternative to AM-agent on performance and intestinal health in broilers under necrotic enteritis(NE)challenge.A total of 960 dayold mixed-sex Cobb 500 chicks were randomly distributed to 48-floor pens with 6 treatments replicated 8 times with 20 birds *** 6 treatments were as follows:UC,unchallenged control;CC,challenged control;PE,challenged group plus PE;AM,challenged group plus AM;FAP,challenged group plus a full dose of AM with PE;HAP,challenged group plus a half dose of AM with PE in starter,grower and finisher *** in the challenged groups were inoculated with Eimeria *** d 9 and Clostridium perfringens on d *** body weight gain(BWG),feed intake(FI),feed conversion ratio(FCR),and livability of birds were compromised,and intestinal lesions and mortality were increased(P<0.05)by NE challenge,illustrating a successful clinical NE *** fed AM had higher BWG and FI,and lower FCR,mortality,and intestinal lesions compared to the CC group(P<0.05).Birds fed PE had improved FCR(P<0.05)and livability(5.8%)in an overall period compared to the CC *** d 16,PE supplementation reduced ileal lesion scores in only male birds(P<0.05).Birds fed PE had decreased Eimeria maxima and Eimeria acervulina oocyst counts in caecal content(P<0.05).Birds fed PE had decreased Eimeria brunetti and total oocyst counts in caecal content,and *** oocyst counts in ileal content in only female birds(P<0.05).On d 35,PE supplementation reduced variation of BW in both male and female birds and increased yellowness(b*value,14.4%)in the *** findings suggest the potential of PE supplementation in diets to improve the performance and intestinal health of birds under clinical NE a

关键词： plant extract Performance Alternative to antimicrobials Clinical necrotic enteritis Broiler chicken

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Orientin and vitexin attenuate lipopolysaccharide-induced inflammatory responses in RAW264.7 cells:a molecular docking study,biochemical characterization,and mechanism analysis

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Food Science and Human Wellness 2022年第5期11卷 1273-1281页

作者： Yue Yu Fusheng Pei Zhanming Li School of Grain Science and Technology Jiangsu University of Science and TechnologyZhenjiang 212004China

plant flavonoids have received considerable attention for their health ***,structure-activity relationships between flavonoids such as orientin and vitexin with similar structures are rarely *** the present study,molecular docking study suggested that orientin and vitexin suppressed inflammatory responses through the modulation of the mitogen-activated protein kinase(MAPK)signaling ***,RAW264.7 cells with lipopolysaccharide-induced inflammation were used to evaluate the anti-inflammatory activities of orientin and vitexin,based on the inflammation-related cytokines production,quantitative real-time reverse transcription PCR,and western blotting *** a result,orientin or vitexin attenuated inflammatory responses through modulation of the MAPK/NF-κB signaling pathway by suppressing NF-κB translocation.

关键词： Anti-inflammatory Mitogen-activated protein kinase plant extract NF-κB Molecular docking

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