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Enzymatic synthesis of bioactive compounds by Rhus laccase from Chinese Rhus vernicifera

Science China Chemistry 2007年第2期50卷 179-182页

作者： AKIYAMA Kazuhiro MIYAKOSHI Tetsuo Department of Industrial Chemistry School of Science and TechnologyMeiji UniversityHigashi-mitaTama-kuKawasaki 214Japan

A simple one step synthesis of pinoresinol and its derivatives-active components of Du-Zhong(Eu-commia ulmoides) -from coniferyl alcohol and p-courmaryl alcohol with higher yields was achieved by Rhus laccases(RL) catalysis in water miscible organic solvents. Biomacromolecules dehydro-genative polymers(DHP) were only synthesized by fungal laccases,not by RL. The structures and the reaction mechanism were discussed to promote the understanding of the function of laccases in the process of lignin biosynthesis.

关键词： lignan laccases pinoresinol dehydrogenative polymers enzymatic mechanism

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Quantitative Assessment of Different Phenolic Compounds in Texas Olive Oils versus Foreign Oils

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Journal of Agricultural Science and Technology 2010年第5期4卷 61-67页

作者： N. S. A. Malik J. L. Perez United States Department of Agriculture Agricultural Research Service 2413 E. Hwy83 Weslaco TX 78596 USA

The purpose of this study was to evaluate the quality of the first time commercially produced Texas olive oil. Thus, quantitative assessments of different phenolic compounds in Texas olive versus foreign oils were performed using modern analytical techniques. Samples of commercial and laboratory extracted Texas olive oils were compared with sample from 11 foreign oils. Total polyphenols in each oil sample were measured by the Folin-Ciocalteau method. Individual polyphenols were separated by high performance liquid chromatography and identified by comparing retention time and UV spectra with known compounds. Free acidity and the UV absorption were measured by standard AOCS （American Oil Chemists Society） methods. The Texas olive oil contained 27% higher amounts of total phenols than the highest amount of a foreign oil sample. Similarly, Texas olive oil also contained higher amounts ofoleuropein, hydroxytyrosol, and pinoresinol compared to all other oils tested. Thus, olive oil pressed from olives harvested in hot summer days will be very competitive with other oils with regard to polyphenols contents and hence its health benefits. The free acidity in the commercial Texas oil was 0.35%, based on oleic acid, and its coefficient at K268 UV absorption was 〈0.166. These numbers affirm that the Texas olive is ＂Extra Virgin＂.

关键词： Oleuropein olive oil pinoresinol polyphenols tyrosol.

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Enzymatic Preparation of Bioactive Compounds by Rhus Laccases

Enzymatic Preparation of Bioactive Compounds by Rhus Laccase...

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中国化学会第28届学术年会

作者： Tetsuo Miyakoshi Department of Industrial Chemistry Meiji University

＜正＞Monolignols （lignin precursors） were catalyzed by Rhus laccases （RL） in organic solvent-water mixture. A simple one-step synthesis of bioactive pinoresinol and its derivatives, lignin and lignan model compounds from coniferyl alcohol and p-courmaryl alcohol etc. with higher yields was achieved by RL catalysis in water miscible organic

关键词： Rhus laccase lignin lignan monolignol pinoresinol dehydrogenative polymer(DHP)

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