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A novel protease-activated receptor 1 inhibitor from the leech Whitmania pigra

Chinese Journal of Natural Medicines 2019年第8期17卷 591-599页

作者： REN Shen-Hong LIU Zhao-Jun CAO Yuan HUA Yi CHEN Cong GUO Wei KONG Yi School of Life Science & Technology China Pharmaceutical University Nanjing 210009 China School of Life Science China Pharmaceutical University Nanjing 210009 China Jiangsu Province Hospital of Chinese Medicine Affiliated Hospital of Nanjing University of Chinese Medicine Nanjing 210029 China

Whitmania pigra has been used as a traditional Chinese medicine(TCM) for promoting blood circulation, alleviating blood coagulation, activating meridians and relieving stasis for several hundred years. However, the therapeutic components of this species, especially proteins and peptides were poorly exploited. Until now only a few of them were obtained by using chromatographic isolation and purification. In recent decade, transcriptome techniques were rapidly developed, and have been used to fully reveal the functional components of many animal venoms. In the present study, the c DNA of the salivary gland of Whitmania pigra was sequenced by illumina and the transcriptome was assembled by using Trinity. The proteome were analysed by LC-MS/MS. Based on the data of the transcriptome and the proteome, a potential antiplatelet protein named pigrin was found. Pigrin was cloned and expressed using P. pastoris GS115. The antiplatelet and antithrombotic bioactivities of pigrin were tested by using aggregometer and the rat arterio-venous shunt thrombosis model, respectively. The bleeding time of pigrin was measured by a mice tail cutting method. The docking of pigrin and protease-activated receptor 1(PAR1) or collagen were conducted using the ZDOCK Server. Pigrin was able to selectively inhibit platelet aggregation stimulated by PAR1 agonist and collagen. Pigrin attenuated thrombotic formation in vivo in rat, while did not prolong bleeding time at its effective dosage. There are significant differences in the key residues participating in binding of Pigrin-Collagen complex from Pigrin-PAR1 complex. In conclusion, a novel PAR1 inhibitor pigrin was found from the leech Whitmania pigra. This study helped to elucidate the mechanism of the leech for the treatment of cardiovascular disorder.

关键词： Whitmania pigra Transcriptome and proteome PAR1 inhibitor Thrombosis

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Marked electrical synapses on AP neuron of the leech (Whitmania pigra)

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Chinese Science Bulletin 1998年第16期43卷 1376-1380页

作者： Duanwen Shen Li Wang Renji Zhang Peking Univ Coll Life Sci Dept Physiol & Biophys Beijing 100871 Peoples R China

An initial attempt reveals parameters of marked electric synapse on AP perceptive neuron of leech. The width of gap between marked AP and either neuro- or glio-process is 2-3 nm, the diameter of the hexagonal array of connexion is 8-9 nm with a central pole of 2-2.5 nm, the same as the structure of annular lamellar bodies internalized by AP-glia processes in the AP.

关键词： Whitmania pigra AP neuron marked gap junction glial cell annular lamellar body.

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THE MORPHOLOGICAL SIMILARITY AND SYMMETRY OF NEURONES IN THE LEECH Whitmania pigra

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Science China Chemistry 1990年第7期33卷 821-827页

作者：王丹冰张人骥 Department of Biology Peking University Beijing 100871 PRC

Using the method of intracellular injection of horseradish peroxidase, the arborizations of several neurones with different functions in the segmental ganglia of the leech Whitmania pigra are investigated. Comparison is made in each kind of neurones in order to verify their typical pattern. Results show that the morphology of the axonal arbors of identified neurones has an accurate similarity within and among the individuals of the leech, and the axonal arborizations of the bilaterally identified neurones in the same ganglion have a stable symmetry. The possible reasons of the formation and its functional significance of the symmetrical nervous system are discussed.

关键词： Whitmania pigra identified neur ones horseradish peroxidase axonal symmetry similarity of the arborizations

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放养时间段对宽体金线蛭产茧率的影响

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河北渔业 2019年第8期 32-33,55页

作者：舒斌董扬帆郝晨光李军涛张秀霞冼健安周小云王冬梅华中农业大学水产学院湖北武汉430070 中国热带农业科学院热带生物技术研究所海南海口571101 海南省海洋生物资源功能性成分研究与利用重点实验室海南海口571101

为探究不同放养时间段对宽体金线蛭(Whitmania pigra Whitman)繁殖时产茧率的影响,找到繁殖时最佳需要放养的时间,选用体重规格为20.00 g±2.00 g的宽体金线蛭亲本,按5 d间隔设放养时间不同的7个试验组,其放养时间分别为10 d、15 d、20 ... 详细信息

为探究不同放养时间段对宽体金线蛭(Whitmania pigra Whitman)繁殖时产茧率的影响,找到繁殖时最佳需要放养的时间,选用体重规格为20.00 g±2.00 g的宽体金线蛭亲本,按5 d间隔设放养时间不同的7个试验组,其放养时间分别为10 d、15 d、20 d、25 d、30 d、35 d、40 d,将亲本放养于聚乙烯泡沫箱中,观察其繁殖产茧及孵化情况。结果表明:30 d及35 d组产茧率最高,分别为11.71%和14.63%;10 d组产茧率最低,为3.97%;40 d组孵化率最低为95.38%;其余组都为100%。因此,放养繁殖时间在30~35 d时产茧率和孵化率较好,经济效益最佳。

关键词：宽体金线蛭(Whitmania pigra Whitman) 放养时间段产茧率孵化率

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