检索结果-南通市图书馆

phytochrome B and AGB1 Coordinately Regulate Photomorphogenesis by Antagonistically Modulating PIF3 Stability in Arabidopsis

引用

Molecular Plant 2019年第2期12卷 229-247页

作者： Pengbo Xu Hongli Lian Feng Xu Ting Zhang Sheng Wang Wenxiu Wang Shasha Du Jirong Huang Hong-Quan Yang School of Life Sciences and Biotechnology Shanghai Jiao Tong University Shanghai 200240 China School of Agriculture and Biology Shanghai Jiao Tong University Shanghai 200240 China State Key Laboratory of Genetic Engineering Collaborative Innovation Center for Genetics and Development School of Life Sciences Fudan University Shanghai 200438 China Shanghai Key Laboratory of Plant Molecular Sciences College of Life Sciences Shanghai Normal University Shanghai 200234 China These authors contributed equally to this article.

phytochrome B (phyB), the primary red light photoreceptor, promotes photomorphogenesis in Arabidopsis by interacting with the basic helix-loop-helix transcriptional factor PIF3 and inducing its phosphorylation and degradation. Heterotrimeric G proteins are known to regulate various developmental processes in plants and animals. In Arabidopsis, the G-protein β subunit AGB1 is known to repress photomorphogenesis. However, whether and how phyB and AGB1 coordinately regulate photomorphogenesis are largely unknown. Here we show that phyB physically interacts with AGB1 in a red light-dependent manner and that AGB1 interacts directly with PIF3. Moreover, we demonstrate that the AGB1-PIF3 interaction inhibits the association of PIF3 with phyB, leading to reduced phosphorylation and degradation of PIF3, whereas the phyB-AGB1 interaction represses the association of PIF3 with AGB1, resulting in enhaneed phosphorylation and degradation of PIF3. Our results suggest that phyB and AGB1 antagonistically regulate PIF3 stability by dynamically interacting with each other and PIF3. This dynamic mechanism may allow plants to balanee phyB and G-protein signaling to optimize photomorphogenesis.

关键词： Arabidopsis phytochrome B heterotrimeric G-protein β sub unit AGB1 PIF3 photomorphogenesis protein stability

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

A Rice phytochrome A in Arabidopsis： The Role of the N-terminus under red and far-red light

引用

Molecular Plant 2008年第1期1卷 84-102页

作者： Julia Kneissl Tomoko Shinomura Masaki Furuya Cordelia Bolle Ludwig-Maximilians-Universitat Munchen Bereich Botanik Menzinger Str. 67 80638 Munchen Germany Hitachi Advanced Research Laboratory Hatoyama Saitama 350-0395 Japan

The phytochrome （phy）A and phyB photoreceptors mediate three photobiological response modes in plants; whereas phyA can mediate the very-low-fluence response （VLFR）, the high-irradiance response （HIR） and, to some extent, the low fluence response （LFR）, phyB and other type U phytochromes only mediate the LFR. To investigate to what level a rice phyA can complement for Arabidopsis phyA or phyB function and to evaluate the role of the serine residues in the first 20 amino acids of the N-terminus of phyA, we examined VLFR, LFR, and HIR responses in phyB and phyAphyB mutant plants transformed with rice PHYA cDNA or a mutant rice PHYA cDNA in which the first 10 serine residues were mutated to alanines （phyA SA）. Utilizing mutants without endogenous phyB allowed the evaluation of red-light-derived responses sensed by the rice phyA. In summary, the WT rice phyA could complement VLFR and LFR responses such as inhibition of hypocotyl elongation under pulses of FR or continuous R light, induction of flowering and leaf expansion, whereas the phyA SA was more specific for HIR responses （e.g. inhibition of hypocotyl elongation and anthocyanin accumulation under continuous far-red light）. As the N-terminal serines can no longer be phosphorylated in the phyA SA mutant, this suggests a role for phosphorylation discriminating between the different phyA-dependent responses. The efficacy of the rice phyA expressed in Arabidopsis was dependent upon the developmental age of the plants analyzed and on the physiological response, suggesting a stage-dependent downstream modulation of phytochrome signaling.

关键词： phytochrome A N-Terminus phytochrome KCNJ4 gene ITLN1 gene Arabidopsis far red light VLFR

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Effects of G protein and cGMP on phytochrome-mediated amaranthin synthesis in Amaranthus caudatus seedlings

引用

Science China(Life Sciences) 1998年第3期41卷 232-237页

作者：周君莉马力耕孙大业 1. Laboratory of Molecular Cell Biology Hebei Normal University 050016 Shijiazhuang China

The effects of G protein and cGMP on phytochrome mediated amaranthin biosynthesis in Amaranthus caudatus seedlings were studied. It was shown that G protein agonist cholera toxin induced amaranthin synthesis in darkness, whereas G protein antagonist pertussis toxin inhibited red light induced amaranthin synthesis. Amaranthin synthesis was also induced by exogenous cGMP, while the amaranthin biosynthesis induced by cholera toxin, red light and exogenous cGMP was inhibited by genistein. LY 83583, an inhibitor of guanylyl cyclase, inhibited the amaranthin synthesis induced both by red light and cholera toxin, while it was not able to inhibit the amaranthin synthesis induced by exogenous cGMP. These results suggest that G protein, guanylyl cyclase and cGMP were the candidates in phytochrome signal transduction chain for red light induced amaranthin biosynthesis and the red light signal transduction chain might be as follows: red light→phytochrome→G protein→guanylyl cyclase→cGMP.

关键词： G protein cGMP phytochrome Amaranthus caudatus amaranthin biosynthesis signal transduction.

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Stress responsive gene CIPK14 is involved in phytochrome A-mediated far-red light inhibition of greening in Arabidopsis

引用

Science China(Life Sciences) 2010年第11期53卷 1307-1314页

作者： QIN YuZhi2,3, GUO Ming1, LI Xu1, XIONG XingYao2,3, HE ChangZheng2,3, NIE XianZhou4 & LIU XuanMing1 1College of Life Science and Biotechnology, Hunan University, Changsha 410082, China 2College of Horticulture and Landscape, Hunan Agricultural University, Changsha 410128, China 3Hunan Provincial Key Laboratory of Crop Germplasm Innovation and Utilization, Hunan Agricultural University, Changsha 410128, China 4Potato Research Centre, Agriculture and Agri-Food Canada, Fredericton, New Brunswick E3B 4Z7, Canada 2. College of Horticulture and Landscape Hunan Agricultural University Changsha 410128 China3. Hunan Provincial Key Laboratory of Crop Germplasm Innovation and Utilization Hunan Agricultural University Changsha 410128 China1. College of Life Science and Biotechnology Hunan University Changsha 410082 China4. Potato Research Centre Agriculture and Agri-Food Canada Fredericton New Brunswick E3B 4Z7 Canada

In this study, we show that CIPK14,a stress responsive CBL-interacting protein kinase gene,is involved in phytochrome A-mediated far-red light inhibition of greening in Arabidopsis seedlings. The CIPK14-impairment mutant cipk14 grown in continuous far-red (FR) light did not show greening when exposed to white light illumination for 15 h. By contrast, the FR-grown phytochrome A null mutant phyA greened within 0.5 h of exposure to white light. Although greening of Col-4 (wild-type) was not completely abolished by FR, it exhibited a significantly decreased greening capacity compared with that of phyA. Further analyses demonstrated that the expression of protochlorophyllide reductase (POR) genes was correlated with the greening ability of the genotypes. In addition, CIPK14 appeared to be regulated by both the circadian clock and PhyA. Taken together, these results suggest that CIPK14 plays a role in PhyA-mediated FR inhibition of seedling greening, and that a Ca-related kinase may be involved in a previously undefined branch point in the phytochrome A signaling pathway.

关键词： chlorophyll CIPK14 far-red light inhibition of greening POR phytochrome A

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Arabidopsis FHY1 and FHY1-LIKE Are Not Required for phytochrome A Signal Transduction in the Nucleus

引用

Plant Communications 2020年第2期1卷 34-44页

作者： Chiara Menon Cornelia Klose Andreas Hiltbrunner Center for Plant Molecular Biology University of Tubingen72076 TubingenGermany Faculty of Biology Institute of Biology IIUniversity of Freiburg79104 FreiburgGermany Signalling Research Centres BIOSS and CIBSS University of Freiburg79104 FreiburgGermany

Photoreceptors of the phytochrome family control a multitude of responses in *** A(phyA)is essential for far-red light perception,which is important for germination and seedling establishment in strong canopy *** of phyA from the cytosol into nucleus is a key step in farred light signaling and requires FAR-RED ELONGATED HYPOCOTYL 1(FHY1)and FHY1-LIKE(FHL).FHY1/FHL bind to phyA downstream signaling ***,it has been suggested that FHY1/FHL also have a function in assembling phyA transcription factor complexes in the ***,in this study,we show that constitutively nuclear-localized phyA is active in the absence of FHY1 and ***,an artificial FHY1,consisting of an SV40 NLS,a phyA binding site,and a YFP tag as spacer between them,complements the fhy1-3 fhl-1 double *** findings show that FHY1 and FHL are not required for phyA downstream signaling in the ***,we found that lines expressing phyA-NLS-YFP are hypersensitive to red and far-red light and that slightly increased levels of constitutively nuclear-localized phyA result in photomorphogenic development in the ***,restricting phyA to the cytosol and inducing nuclear transport in light by interaction with FHY1/FHL might be important to suppress photomorphogenesis in the dark.

关键词： phytochrome phyA FHY1 FHL nuclear transport far-red light

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Effect of calcium on phytochrome-mediated synthesis of amaranthin in Amaranthus caudatus seedlings

引用

Chinese Science Bulletin 1995年第17期40卷 1469-1473页

作者：马力耕周君莉安宜崔国军孙大业 HidakaHiroyoshi Hidaka Hiroyoshi Department of Biology Hebei Normal University. Shijiazhuang 050016 China) Department of Pharmacology College of Medicine Nagoya University Nagoya 466. Japan)

Light plays a critical role in the regulation of plant morphogenetic and physiologicalresponses. As one of the extracellular signals, light signa1 is first received by the plantcells, then it affects the physiological and biochemical status of the cells and the expressionof genetic characters through signal transduction. Tong has pointed out that until recentlythere had been little progress in the intersteps between phytochrome activation and

关键词： calcium phytochrome Amaranthus caudatus amaranthin synthesis.

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

OsFKBP1 interacts with phytochrome B in rice

引用

Progress in Natural Science:Materials International 2009年第11期19卷 1523-1528页

作者： Suhai Gu,Fang Zhang,Naihu Wu~* Institute of Genetics and Developmental Biology,Chinese Academy of Sciences,3 Zhongguancunnanyitiao,Beijing 100080,China Institute of Genetics and Developmental Biology Chinese Academy of Sciences 3 Zhongguancunnanyitiao Beijing 100080 China

Part of the C-terminal of rice phytochrome B was used as bait to screen a cDNA library constructed from young rice panicles in a yeast two-hybrid screening.A positive clone was selected,and the sequence was characterized as encoding a FKBP protein,which is a putative developmental *** in vitro pull-down assay using recombinant proteins and analysis of bimolecular fluorescence complementation in onion epidermal cells indicated that two proteins interact in vitro and in *** results of reverse transcription polymerase chain reaction showed that two genes were co-expressed in the examined rice organs,suggesting that OsFKBP1 may play a role in light signal transduction in rice.

关键词： Rice Yeast two hybrid phytochrome BiFC

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

The sulfur metabolism regulator MetR is a global regulator controlling phytochrome-dependent light responses in Aspergillus nidulans

引用

Science Bulletin 2021年第6期66卷 592-602,M0004页

作者： Zhenzhong Yu Jia Gao Olumuyiwa Igbalajobi Marek Skoneczny Marzena Sienko Agnieszka M.Maciejewska Jerzy Brzywczy Reinhard Fischer Department of Microbiology Institute for Applied BiosciencesKarlsruhe Institute of Technology(KIT)-South CampusKarlsruhe D-76131Germany Key Laboratory of Plant Immunity Jiangsu Provincial Key Lab for Organic Solid Waste UtilizationJiangsu Collaborative Innovation Center for Solid Organic Waste Resource UtilizationNational Engineering Research Center for Organic-based FertilizersNanjing Agricultural UniversityNanjing 210095China Institute of Biochemistry and Biophysics Polish Academy of SciencesWarszawa 02-106Poland Michael Smith Laboratories Department of Microbiology and ImmunologyUniversity of British ColumbiaVancouverBritish Columbia VGT 1Z4Canada

phytochrome-dependent light signaling has been studied in several *** Aspergillus nidulans lightstimulated phytochrome activates the high-osmolarity glycerol(HOG)signaling pathway and thereby controls the expression of a large number of genes,many of which are related to stress *** a genome-wide expression analysis in *** we found that phytochrome,fph A,is under strict expression control of the central regulator of the sulfur-starvation response,Met *** transcriptional regulator is required for the expression of genes involved in inorganic sulfur *** the presence of organic sulfur,Met R is probably ubiquitinated and possibly degraded and the transcription of sulfur-assimilation genes,e.g.,sulfate permease,is turned *** expression analysis described here revealed,however,that Met R additionally controls the expression of hundreds of genes,many of which are required for secondary metabolite *** also show that met R mutation phenocopies fph A deletion,and five other histidine-hybrid kinases are down-regulated in the met R1 ***,we found that light and phytochrome regulate the expression of at least three carbon–sulfur *** work is a further step towards understanding the interplay between light sensing and metabolic pathways.

关键词： phytochrome Sulfur metabolism Transcriptome Cys3 Met4

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Strigolactone-Regulated Hypocotyl Elongation Is Dependent on Cryptochrome and phytochrome Signaling Pathways in Arabidopsis

引用

Molecular Plant 2014年第3期7卷 528-540页

作者： Kun-Peng Jia Qian Luo Sheng-Bo He Xue-Dan Lu Hong-Quan Yang Key Laboratory of Urban Agriculture (South) Ministry of Agriculture and School of Agricultural and Biological Sciences Shanghai Jiaotong University800 Dongchuan Road Shanghai 200240 China Schoolof Life Sciences and Biotechnology Shanghai Jiaotong University. 800 Dongchuan Road Shanghai 200240 China

Seedling development including hypocotyl elongation is a critical phase in the plant life cycle. Light regula- tion of hypocotyl elongation is primarily mediated through the blue light photoreceptor cryptochrome and red/far-red light photoreceptor phytochrome signaling pathways, comprising regulators including COP1, HY5, and phytochrome- interacting factors （PIFs）. The novel phytohormones, strigolactones, also participate in regulating hypocotyl growth. However, how strigolactone coordinates with light and photoreceptors in the regulation of hypocotyl elongation is largely unclear. Here, we demonstrate that strigolactone inhibition of hypocotyl elongation is dependent on cryp- tochrome and phytochrome signaling pathways. The photoreceptor mutants cry1 cry2, phyA, and phyB are hyposensi- tive to strigolactone analog GR24 under the respective monochromatic light conditions, while cop1 and pifl pif3 pif4 pif5 （pifq） quadruple mutants are hypersensitive to GR24 in darkness. Genetic studies indicate that the enhanced respon- siveness of cop1 to GR24 is dependent on HY5 and MAX2, while that of pifq is independent of HY5. Further studies demonstrate that GR24 constitutively up-regulates HY5 expression in the dark and light, whereas GR24-promoted HY5 protein accumulation is light- and cryptochrome and phytochrome photoreceptor-dependent. These results suggest that the light dependency of strigolactone regulation of hypocotyl elongation is likely mediated through MAX2-dependent promotion of HY5 expression, light-dependent accumulation of HY5, and PIF-regulated components.

关键词： hypocotyl elongation cryptochrome phytochrome strigolactones COP1 HY5 PIF MAX2.

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Red Light-Induced phytochrome Relocation into the Nucleus in Adiantum capillus-veneris

引用

Molecular Plant 2012年第3期5卷 611-618页

作者： Hidenori Tsuboi Sachihiko Nakamura Eberhard Schaifer Masamitsu Wadaa Kyushu University Hakozaki 6-10-1 Fukuoka 812-8581 Japan Tokyo Metropolitan University Minami-Osawa 1-1 Hachioji Tokyo 192-0397 Japan Biologie II/Institut f6r Botanik University of Freiburg D-79104 Freiburg Germany Freiburg Institute for Advanced Studies School of Life Sciences Freiburg Germany

phytochromes in seed plants are known to move into nuclei in a red light-dependent manner with or without interacting factors. Here, we show phytochrome relocation to the nuclear region in phytochrome-dependent Adiantum capillus-veneris spore germination by partial spore-irradiation experiments. The nuclear or non-nuclear region of imbibed spores was irradiated with a microbeam of red and/or far-red light and the localization of phytochrome involved in spore germination was estimated from the germination rate. The phytochrome for spore germination existed throughout whole spore under darkness after imbibition, but gradually migrated to the nuclear region following red light irradiation. In- tracellular distribution of PHY-GUS fusion proteins expressed in germinated spores by particle bombardment showed the migration of Acphy2, but not Acphyl, into nucleus in a red light-dependent manner, suggesting that Acphy2 is the photoreceptor for fern spore germination.

关键词： Adiantum capillus-veneris far-red light microbeam phytochrome red light spore germination

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：