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physiological,transcriptome and gene functional analysis provide novel sights into cadmium accumulation and tolerance mechanisms in kenaf

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Journal of Environmental Sciences 2024年第3期137卷 500-514页

作者： Shan Cao Meng Wang Jiao Pan Dengjie Luo Samavia Mubeen Caijin Wang Jiao Yue Xia Wu Qijing Wu Hui Zhang Canni Chen Muzammal Rehman Sichen Xie Ru Li Peng Chen Guangxi Key Laboratory of Agro-environment and Agric-products safety Key Laboratory of Plant Genetics and BreedingCollege of AgricultureGuangxi UniversityNanning 530004China State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources College of Life Science and TechnologyGuangxi UniversityNanning 530004China

Kenaf is considered to have great potential for remediation of heavy metals in ***,studies on molecular mechanisms of root Cd accumulation and tolerance are still *** this study,two differently tolerant kenaf cultivars were selected as materials and the physiological and transcriptomic effects were evaluated under Cd *** study showed that 200μmol/L CdCl_(2) treatment triggered the reactive oxygen species(ROS)explosion and membrane lipid *** with the Cd-sensitive cultivar‘Z',the Cd-tolerant cultivar‘F'was able to resist oxidative stress in cells by producing higher antioxidant enzyme activities and increasing the contents of ascorbic acid(AsA)and glutathione(GSH).The root cell wall of‘F'exhibited higher polysaccharide contents under Cd treatment,providing more Cd-binding *** were 3,439 differentially expressed genes(DEGs)that were co-regulated by Cd treatment in two *** biosynthesis and plant hormone signal transduction pathways were significantly enriched by functional annotation *** associated with pectin,cellulose,and hemi-cellulose metabolism were involved in Cd chelation of root cell wall;V-ATPases,ABCC3 and Narmp3 could participated in vacuolar compartmentalization of Cd;PDR1 was responsible for Cd effux;the organic acid transporters contributed to the absorption of Cd in *** genes might have played key roles in kenaf Cd tolerance and Cd ***,HcZIP2 was identified to be involved in Cd uptake and transport in *** findings provide a deeper understanding of the molecular pathways underlying Cd accumulation and detoxification mechanisms in kenaf.

关键词： Kenaf Cd stress physiological Transcriptome HcZIP2

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physiological and genome-wide gene expression analyses of cold-induced leaf rolling at the seedling stage in rice(Oryza sativa L.)

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The Crop Journal 2019年第4期7卷 431-443页

作者： Jingfang Dong Junliang Zhao Shaohong Zhang Tifeng Yang Qing Liu Xingxue Mao Hua Fu Wu Yang Bin Liu Rice Research Institute Guangdong Academy of Agricultural Sciences/Guangdong Provincial Key Laboratory of New Technology in Rice Breeding

Leaf rolling and discoloration are two chilling-injury symptoms that are widely used as indicators for the evaluation of cold tolerance at the seedling stage in rice. However, the difference in cold-response mechanisms underlying these two traits remains unknown. In the present study, a cold-tolerant rice cultivar, Lijiangxintuanheigu, and a cold-sensitive cultivar, Sanhuangzhan-2, were subjected to low-temperature treatments and physiolog-ical and genome-wide gene expression analyses were conducted. Leaf rolling occurred at temperatures lower than 11℃, whereas discoloration appeared at moderately low temperatures such as 13℃. Chlorophyll contents in both cultivars were significantly decreased at 13℃, but not altered at 11℃. In contrast, the relative water content and relative electrolyte leakage of both cultivars decreased significantly at 11℃, but did not change at 13℃. Expression of genes associated with calcium signaling and abscisic acid (ABA) degradation was significantly altered at 11℃ in comparison with 25℃ and 13℃. Numerous genes in the DREB, MYB, bZIP, NAC, Zinc finger, bHLH, and WRKY gene families were differentially expressed. Many aquaporin genes and the key genes in trehalose and starch synthesis were down regulated at 11℃ in comparison with 25℃ and 13℃. These results suggest that the two chilling injury symptoms are temperature-specific and are controlled by different mechanisms. Cold-induced leaf rolling is associated with calcium and ABA signaling pathways and is regulated by multiple transcriptional regulators. The suppression of aquaporin genes and reduced accumulation of soluble sugars under cold stress results in a reduction in cellular water potential and consequently leaf rolling.

关键词： Rice(Oryza sativa L.) Cold-induced leaf yellowing Cold-induced leaf rolling physiological analysis Genome-wide expression profiling

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physiological effects of weightlessness: countermeasure system development for a long-term Chinese manned spaceflight

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Frontiers of Medicine 2019年第2期13卷 202-212页

作者： Linjie Wang Zhili Li Cheng Tan Shujuan Liu Jianfeng Zhang Siyang He Peng Zou Weibo Liu Yinghui Li State Key Laboratory of Space Medicine Fundamentals and Application China Astronaut Research and Training CenterBeijing 100094China

The Chinese space station will be built around 2020. As a national space laboratory, it will offer unique opportunities for studying the physiological effects of weightlessness and the efficacy of the countermeasures against such effects. In this paper, we described the development of countermeasure systems in the Chinese space program. To emphasize the need of the Chinese space program to implement its own program for developing countermeasures, we reviewed the literature on the negative physiological effects of weightlessness, the challenges of completing missions, the development of countermeasure devices, the establishment of countermeasure programs, and the efficacy of the countermeasure techniques in American and Russian manned spaceflights. In addition, a brief overview was provided on the Chinese research and development on countermeasures to discuss the current status and goals of the development of countermeasures against physiological problems associated with weightlessness.

关键词： countermeasure physiological effects of weightlessness effect evaluation long-term manned spaceflight

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physiological and molecular responses to cold stress in rapeseed(Brassica napus L.)

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Journal of Integrative Agriculture 2019年第12期18卷 2742-2752页

作者： YAN Lei Tariq Shah CHENG Yong Lü Yan ZHANG Xue-kun ZOU Xi-ling Oil Crops Research Institute Chinese Academy of Agricultural Sciences/Key Laboratory of Biology and Genetic Improvement of Oil CropsMinistry of Agriculture and Rural AffairsWuhan 430062P.R.China College of Plant Science and Technology Huazhong Agricultural UniversityWuhan 430070P.R.China

Low temperature is one of the most important abiotic factors inhibiting growth, productivity, and distribution of rapeseed(Brassica napus L.). Therefore, it is important to identify and cultivate cold-tolerant germplasm. The objective of this study was to figure out the mechanism of chilling(4 and 2°C) and freezing(–2 and –4°C) stresses along with a control(22°C) in B. napus cultivars(1801 and C20) under controlled environment(growth chamber). The experiment was arranged in a complete randomized design with three replications. Our results exhibited that under chilling and freezing stresses, the increment of proline accumulation, soluble sugar and protein contents, and antioxidant enzyme activity were enhanced more in 1801 cultivar compared with C20 cultivar. At –2 and –4°C, the seedlings of C20 cultivar died completely compared with 1801 cultivar. Hydrogen peroxide(H2 O2) and malondialdehyde contents(MDA) increased in both cultivars, but when the temperature was decreased up to –2 and –4°C, the MDA and H2 O2 contents continuously dropped in 1801 cultivar. Moreover, we found that leaf abscisic acid(ABA) was enhanced in 1801 cultivar under chilling and freezing stresses. Additionally, the transcriptional regulations of cold-tolerant genes(COLD1, CBF4, COR6.6, COR15, and COR25) were also determined using real-time quantitative PCR(RT-q PCR). RT-q PCR showed that higher expression of these genes were found in 1801 as compared to C20 under cold stress(chilling and freezing stresses). Therefore, it is concluded from this experiment that 1801 cultivar has a higher ability to respond to cold stress(chilling and freezing stresses) by maintaining hormonal, antioxidative, and osmotic activity along with gene transcription process than C20. The result of this study will provide a solid foundation for understanding physiological and molecular mechanisms of cold stress signaling in rapeseed(B. napus).

关键词： Brassica napus L. cold stress morphological features molecular regulation physiological indicators

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The influence of physiological environment on the targeting effect of aptamer-guided gold nanoparticles

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Nano Research 2019年第1期12卷 129-135页

作者： Ding Ding Yinling Zhang Edward A.Sykes Long Chen Zhuo Chen Weihong Tan Molecular Science and Biomedicine Laboratory(MBL) State Key Laboratory of Chemo/Bio-Sensing and ChemometricsCollege of Chemistry and Chemical Engineering and College of Life SciencesHunan UniversityChangsha 410082China School of Medicine Queens University81 Barrie StreetKingstonONK7L 3N6Canada Faculty of Science and Technology University of MacaoAvenida da UniversidadeMacao Taipa 999078China Department of Chemistry Departments of Physiology and Functional GenomicsCenter far Research at the Bio/Nano InterfaceUF Health Cancer CenterUF Genetics Institute and McKnight Brain InstituteUniversity of FloridaGainesvilleFlorida 32611USA Institute of Molecular Medicine Renji HospitalShanghai Jiao Tong University School of MedicineShanghai Jiao Tong UniversityShanghai 200240China

Aptamer guided nano medicine shows great promise in targeted cancer *** the loss of targeting capacity duri ng in vivo or clinical trials has largely hindered its popularity and there are no systematic studies to elucidate the ***,we investigated such loss of targeting capacity by examining how the physiological milieu affected targeting *** functionalized gold nanoparticle(AuNP)was chosen as the model and exposed to human blood serum that is used to mimic physiological *** light scattering(DLS),flow cytometry and label-free liquid chromatography tan dem mass spectrometry(LC-MS/MS)were employed to determi ne variations of NPs'surface chemistry and biological identities changes after serum *** showed that the targeting ability loss was caused by protein corona blocking,replacement and enzymatic cleavage of surface aptamer targeting ***,the aggregation issue is critical for the smaller *** of the protein corona profile indicated the accumulation of immune-related proteins on the surface of aptamer-conjugated NPs,which could induce immune response,resulting in rapid clearanee of NPs.

关键词： targeting effect aptamer nanomedicine physiological environment

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Flexible Piezoresistive Sensors based on Conducting Polymer-coated Fabric Applied to Human physiological Signals Monitoring

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Journal of Bionic Engineering 2020年第1期17卷 55-63页

作者： Xiuzhu Lin Tong Zhang Junhou Cao Han Wen Teng Fei Sen Liu Rui Wang Hui Ren Hongran Zhao State Key Laboratory on Integrated Optoelectronics College of Electronic Science and EngineeringJilin UniversityChangchun 130012China State Key Laboratory of Transducer Technology Shanghai 200050China Department of Food Science and Engineering College of Food Science and EngineeringJilin UniversityChangchun 130062China

This paper describes a flexible pressure sensor based on polypyrrole(PPy)-Cotton composites,in which PPy is grown on cellulose fibers of cotton pads via an in situ vapor growth method,which is beneficial to the homogeneity of the *** resulting devices exhibits rapid response and recovery speed,the response and recovery times are 220 ms and 240 ms,*** optimal PPy-Cotton Pads(PCPs)sensor shows low detection limit,which is about 50 *** the same time,it exhibits excellent durability in the measurement of repeated loading-unloading pressure over 1000 *** resultant sensor can be attached on different positions of body and applied to recording physiological signals,such as wrist pulse,vocal cord vibration,respiration and eyes ***,a 4×4 pressure sensor array shows that the PCPs sensor has capability in pressure distribution detection and represents great potential in the fields of wearable electronics and biomedical devices.

关键词： flexible device pressure sensor vapor growth physiological signals monit

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Effect of diets on the feeding behavior and physiological properties of suspension-feeding sea cucumber Cucumaria frondosa

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Journal of Oceanology and Limnology 2020年第3期38卷 883-893页

作者： YU Zonghe ROBINSON Shawn MACDONALD Bruce LANDER Terralynn SMITH Craig College of Marine Sciences South China Agricultural UniversityGuangzhou 510642China Fisheries and Oceans Canada St.Andrews Biological StationSt.AndrewsNB E5B 2L9Canada Biology Department and Centre for Coastal Studies and Aquaculture University of New BrunswickSaint JohnNB E2L 4L5Canada

The suspension-feeding sea cucumber Cucumaria frondosa has become commercially important in recent *** proper diets is the first important step for intensive aquaculture of this sea *** this study,adult *** were exposed to one of the following diet treatments:control(no diet provided),two powdered seaweeds(Ascophyllum nodosum and Saccharina latissima),a commercially available microalgal diet(shellfish diet)and natural *** effects of diets on the feeding behavior and physiological properties of sea cucumbers were investigated after a 5-week rearing *** show that sea cucumbers fed with shellfish diet exhibited a significantly higher tentacle insertion rate(1.80±0.20 insertion/min)than these fed with seaweed powders,and there was no significant different between the two groups fed by seaweed *** significant difference was found on the fecal production rate among the feeding *** minimum oxygen consumption rate was observed in the control group(5.76±0.99μg O2/(g·h)),which is significantly lower than individuals fed with ***,shellfish diet,and natural seston;however,no significant difference was shown between those of control and *** *** maximum ammonium excretion rate was found in the *** group(0.03±0.01μmol/(g·h)),which is significantly higher than other *** minimum O/N ratio was observed in the *** group(14.57±1.04),which is significantly lower than the ***,shellfish diet,and natural seston *** fed with seaweed powders had similar physiological properties with these fed with microalgae diet and natural seston,indicating that *** and *** can be explored as promising diets for intensive aquaculture of ***.

关键词： sea cucumber Cucumaria frondosa physiological property feeding behavior nutritional condition

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Effects of sodium benzoate on growth and physiological characteristics of wheat seedlings under compound heavy metal stress

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Journal of Integrative Agriculture 2020年第4期19卷 1010-1018页

作者： LIANG Pan-pan ZHAO Chen LIN Yuan GENG Ji-jia CHEN Yuan CHEN De-hua ZHANG Xiang Key Laboratory of Crop Genetics and Physiology of Jiangsu Province/Agricultural College Yangzhou UniversityYangzhou 225009P.R.China

In this study, we investigated the effect of exogenous sodium benzoate on wheat seedlings(Yangmai 16) grown under heavy metal stress. The results showed that 2.4 mmol kg-1 of heavy metals significantly inhibited growth and delayed emergence of wheat seedlings. Under compound heavy metal stress, application of 2-4 g L^-1 sodium benzoate significantly increased(P6 g L^-1) inhibited the growth of wheat seedlings and even caused damage to seedlings. Correlation analysis showed that when the sodium benzoate concentration was in the range of 1.97-3.12 g L^-1(2016) and 1.58-3.27 g L^-1(2017), values of chlorophyll and its components, root activity, SOD activity, soluble protein, and free proline content were the highest. When the sodium benzoate concentration was raised to 2.59 g L^-1(2016) or 3.02 g L^-1(2017), MDA content was the lowest. Ultimately, exogenous sodium benzoate(2-4 g L^-1) facilitates root development and improves the root activity of wheat seedlings grown under compound heavy metals stress, thereby effectively alleviating the damage of compound heavy metal stress in wheat seedlings.

关键词： sodium benzoate compound heavy metals wheat seedlings physiological characteristics

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Field identification of morphological and physiological traits in two special mutants with strong tolerance and high sensitivity to drought stress in upland rice(Oryza sativa L.)

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Journal of Integrative Agriculture 2019年第5期18卷 970-981页

作者： HUANG Min XU Yu-hui WANG Hua-qi College of Agronomy and Biotechnology China Agricultural University

The two mutants idr1-1 and 297-28, which were obtained from the radiation mutation of HD297 and IAPAR9, were used as experimental materials in this study for a 2-year(2012 and 2013) experiment about field drought resistance identification in Beijing, China. Key agronomic traits and water-related physiological indexes were observed and measured, including the leaf anti-dead level(LADL), days to heading, plant height, setting percentage, aboveground biomass, leaf water potential(LWP), net photosynthetic rate(Pn) and transpiration rate. The results showed that the mutant idr1-1 that was under drought stress(DS) conditions for 2 years had the highest LADL grades(1.3 and 2.0) among all the materials, and they were 2–3 grades stronger than the wild-type IAPAR9 with an average that was 21.4% higher for the setting percentage than the wild type. Compared with the IAPAR9 for the 2-year average delay in the days to heading and the reduction rates in the plant height, setting percentage, and aboveground biomass under DS compared with the well-watered(WW) treatment, idr1-1 showed 3.2% less delay and 19.1, 16.4, and 6.1% less reduction, respectively. The idr1-1 in the LWP always exhibited the highest performance among all the materials. The Pn of idr1-1 under severe and mild DS comparing with that under WW was slightly decreased and even slightly increased, respectively, leading to an average reduction rate of only 0.92%, which was 26.93% less than that of IAPAR9. Under the severe DS, idr1-1 still showed the highest value of 16.88 μmol CO2 m–2 s–1 among all the materials and was significantly higher than that of IAPAR9(11.66 μmol CO2 m–2 s–1). Furthermore, only idr1-1 had the increased and the highest transpiration rate values(7.6 and 6.04 mmol H2 O m–2 s–1) under both mild and severe DS compared with the values under WW, when the transpiration rate of all the other materials significantly decreased. By contrast, the 297-28 in terms of the LADL grade under DS was the lowest(7.0)

关键词： rice mutants morphological and physiological traits drought resistance field identification

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Effect of physiological heterogeneity of E. coli population on antibiotic susceptivity test

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Science China(Life Sciences) 2007年第6期50卷 808-813页

作者： LIU YuQing1,3, ZHANG HuaiQiang2, SHEN JianZhong3 & GAO PeiJi2 1 Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Science, Jinan 250100, China 2 State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, China 3 College of Veterinary Medicine, China Agricultural University, Beijing 100094, China 1. Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Science Jinan 250100 China 3. College of Veterinary Medicine China Agricultural University Beijing 100094 China 2. State Key Laboratory of Microbial Technology Shandong University Jinan 250100 China

According to the instantaneous growth rate (dN/dt) of E. coli CVCC249 growing in batch culture, the entire growth progress was distinguished into four phases: accelerating growth phase, constant growth phase, decelerating growth phase; declining phase, in each of which obvious variation in physiological; biochemical properties was detected, including total DNA, total protein,; MTT-dehydrogenase activity, etc., that led to difference in their antibiotic susceptivity. Antibiotic susceptivity of the population sampled from each phase was tested by Concentration-killing Curve (CKC) approach following the formula N=N 0/{1+exp[r·(x-BC 50)]}, showing as normal distribution at the individual cell level for an internal population, in which the median bactericidal concentration BC 50 represents the mean level of susceptivity, while the bactericidal span BC 199=(2lnN 0)/r indicates the variation degree of the antibiotic susceptivity. Furthermore, tested by CKC approach, the antibiotic susceptivity of E. coli CVCC249 population in each physiological phase to gentamicin or enoxacin was various: susceptivity of the population in the constant growth phase; declining phase all increased compared with that in the accelerating growth phase for gentamicin but declined for enoxacin. The primary investigations revealed that the physiological phase should be taken into account in the context of antibiotic susceptivity; research into antimicrobial mechanism. However there are few reports concerned with this study. Further research using different kinds of antibiotics with synchronized continuous culture of different bacterial strains is required.

关键词： instantaneous growth rate, batch culture, physiological heterogeneity, concentration-killing curve, antibiotic susceptivity test

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