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Nucleotide Sequence Assessment of Four ORFs of Citrus Tristeza Virus: Evidence of Recombination

Phyton-International Journal of Experimental Botany 2023年第3期92卷 691-705页

作者： Adel A.Rezk Hala A.Amin Department of Agricultural Biotechnology College of Agricultural and Food ScienceKing Faisal UniversityAl-Ahsa31982Saudi Arabia Virus and Phytoplasma Research Department Plant Pathology Research InstituteAgricultural Research Center(ARC)Giza12619Egypt

Citrus Tristeza Virus(CTV),usually occurs in nature as a mixture of *** naturally infected citrus(Citrus sinensis)trees grafted on sour orange rootstock were collected from three citrus growing governorates in Egypt(Sharqia,Qalyubia and Garbia).In this study,RT-PCR,Single-Strand Conformation Polymorphism(SSCP)and nucleotide sequence analysis were used for four independent CTV genomic regions(p65,p18,p20,and p23)to detect and assess the sequence and genetic variabilities among CTV Egyptian *** products(650 bp)for the CTV p23 gene obtained from the selected isolates were used for the SSCP analysis and DNA *** patterns of p23 gene for individual isolates yielded different complex haplotype *** sequence analysis of p23 region amplified from six isolates under study revealed that p23 shared high nucleotide identity 98.7%with T36 isolate from USA,*** analysis of p23 gene indicated a close evolutionary relationship between all examined isolates and Qaha isolate(T36 isolate group),suggesting that they may have originated from closely related *** sequence analysis of the three genes located on CTV 3′-coterminal overhang,p18,p20 and p65,amplified from isolate A3,Sharqia governorate,revealed that the p18,p65,and p20 genes were related to the T3-KB isolate from South Africa with 99%–100%sequence *** relationship analysis for p65,p18 and p20 ORFs clustered the current A3 isolate with T3 genotype *** recombination analysis identified three of six isolates from Sharqia,and Garbia as potential recombinant for p23 *** isolates T36 and T3 were identified as major donors for recombination events in isolate *** results concluded that p23 ORF likely to be as a hotspot region for recombination and originated through recombination *** current study indicated that recombination is an important factor for the origin of CTV strains in Egypt.

关键词： Citrus Tristeza Virus hotspot region phylogenetic relationship analysis sequence comparison SSCP analysis

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