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Synergy and translation of allogenic bone marrow stem cells after photodynamic treatment of rheumatoid arthritis with tetra sulfonatophenyl porphyrin and TiO2 nanowhiskers

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Nano Research 2016年第11期9卷 3305-3321页

作者： Fawad U. Rehman Chunqiu Zhao Changyu Wu Xiaoqi Li Hui Jiang Matthias Selke Xuemei Wang State Key Laboratory of Bioelectronics School of Biological Science and Medical Engineering Southeast University Nanjing 210096 China Department of Chemistry and Biochemistry California State University Los Angeles CA 90032 USA Nanjing Foreign Language School Nanjing 210096 China

Rheumatoid arthritis （RA） etiology and amelioration remains a challenge in modern therapeutics. Herein, we explored the synergistic effect of allogenic bone marrow stem cell （BMSC） translation and photodynamic treatment of RA with tetra sulfonatophenyl porphyrin （TSPP） and TiO2 nanocomposites as a new strategy for RA theranostics. The translation of BMSCs with miRNAs into infected joints in long bones post-photodynamic therapy is helpful for treating and understanding RA pathophysiology. We observed that allogenic BMSC translation combined with TSPP-TiO2 nanocomposites can significantly （p 〈 0.01） lower the concentrations of serum biomarkers （tumor necrosis factor-α and interleukin-17） in a collagen induced arthritis （CIA） murine model, both in vitro and in vivo, as well as improve other parameters such as arthritis score, BMSC count, complete blood count, and numbers of platelets, red blood cells, and white blood cells. Moreover, a fluorescent TSPP in the feet or long bones and X-ray bioimaging of RA joints revealed the clinical efficacy of BMSCs combined with TSPP-TiO2 nanocomposites. Microarray data analysis illustrated that rnomir-375-3p and rno-mir-196b-3p were up-regulated by approximately 100-fold in the BMSCs of ameliorated RA post-photodynamic therapy with TSPP-TiO2 nanocomposites. Our study not only suggests a new approach for RA theranostics, but also helps in understanding RA pathophysiology.

关键词： rheumatoid arthritis allogenic bone marrowstem cells (BMSCs) tetra sulfonatophenyl porphyrin (TSPP)-TiO2nanocomposites photodynamic treatment miRNAs

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Experimental study on the photodynamic treatment of choroidal neovasculization with nanophthaloc-yanine photosensitizer

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International Journal of Ophthalmology(English edition) 2011年第6期4卷 575-581页

作者： Song-Yi Wu Guo-Xing Xu Yi-Ru Peng Xiao-Fang Zhou and Jian Guo Fujian Institute of Ophthalmology the First Affiliated Hospital of Fujian Medical University Fuzhou 350005 Fujian Province China

AIM: To investigate the therapeutic effects of nanophthalocyanine photosensitizers on an experimental rat choroidal neovescularization (CNV) model, as well as to evaluate the cytotoxicity of which on human retinal pigment epithelia (HRPE) and human retinal endothelial cells (HRECs). METHODS: Two types of photosensitizers, G(1)-ZnPc(COOH)(8) and G(1)-ZnPc (COOH)(8)/m respectively, were administrated for photodynamic therapy (PDT) after a successful establishment of CNV model on Brown-Norway (BN) rats via fundus photocoagulation. The therapeutic effects of the two drugs were assessed through optical coherence tomography (OCT), fluorescein fundus angiography (FFA) and transmission electron microscopy (TEM). For cytotoxicity tests, cell counting kit-8 (CCK-8) assays and changes of mitochondrial transmembrane potential (Delta psi m) were conducted on HRPE and HRECs after initial uptake of the two drugs. RESULTS: Both photosensitizers demonstrated an improvement of vascular leakage and closure of CNV 1 week after PDT as confirmed by fundus image, OCT, FFA and TEM. Two weeks after PDT, G(1)-ZnPc (COOH)(8)/m showed a better CNV closure effect versus G(1)-ZnPc(COON)(8)(P0.05). Both photosensitizers showed cytotoxicity on HRPE, but G(1)-ZnPc(COOH)(8)/m induced a lower cell viability. CONCLUSION: G(1)-ZnPc (COOH)(8)/m mediated PDT is better than G(1)-ZnPc (COOH)(8) in CNV closure and also have the advantage of fast metabolism leading to less side effect.

关键词： phthalocyanine carrier nanoparticles photodynamic treatment retina pigment epithelial cells choroidal neovascularization

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