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5-Amino-4-oxopentanoic acid photodynamic diagnosis guided microsurgery and photodynamic therapy on VX2 brain tumour implanted in a rabbit model

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Chinese Medical Journal 2009年第11期122卷 1316-1321页

作者： XIAO Hong LIAO Qiong CHENG Ming LI Fei XIE Bing LI Mei FENG Hua Department of Neurosurgery Southwest Hospital Third Military Medical University Chongqing 400038 China Department of Radiology Southwest Hospital Third Military Medical University Chongqing 400038 China Department of Pathology Sichuan Provincial Cancer Hospital Chengdu Sichuan 610041 China Department of Ophthalmology Research Institute of Surgery Daping Hospital Third Military Medical University Chongqing 400042 China

Background Complete tumour resection is important for improving the prognosis of brain tumour patients. However, extensive resection remains controversial because the tumour margin is difficult to be distinguished from surrounding brain tissue. It has been established that 5-amino-4-0xopentanoic acid （5-aminolevulinic acid, ALA） can be used as a photodynamic diagnostic marker and a photosensitizer for photodynamic therapy in surgical treatment of brain tumours. We investigated the efficacy of ALA photodynamically guided microsurgery and photodynamic therapy on VX2 brain tumour implanted in a rabbit model. Methods Eighty New Zealand rabbits implanted with VX2 brain tumours were randomly assigned to five groups： control, conventional white light microsurgery, a photodynamic therapy group, a photodynamically guided microsurgery group and a group in which guided microsurgery was followed by photodynamic therapy. The VX2 tumour was resected under a surgical microscope. The tumour resection was confirmed with histological analysis. All animals were examined with MRI for presence of any residual tumour tissue. The survival time of each rabbit was recorded. Results All treatment groups showed a significantly extended survival time compared with the control group. photodynamically guided microsurgery combined with photodynamic therapy significantly prolonged survival time, compared with guided microsurgery alone. MRI and the autopsy results confirmed removal of most of the tumours. Conclusions Our results suggest that photodynamically guided surgery and photodynamic therapy significantly reduce or delay local recurrence, increase the effectiveness of radical resection and prolong the survival time of tumour bearing rabbits. Their combination has the potential to be used as a rapid and highly effective treatment of metastatic brain tumours.

关键词： VX2 tumour model 5-amino-4-oxopentanoic acid photodynamic diagnosis photodynamic therapy mean survival time

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photodynamic diagnostics of early gastric cancer:Complexity measures of gastric microcirculation and new model of metastatic adenocarcinoma of rat stomach

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Journal of Innovative Optical Health Sciences 2019年第2期12卷 64-73页

作者： Alexey Pavlov Ekaterina Borisova Olga Pavlova Ilana Agranovich Alexander Khorovodov Andrey Terskov Aysel Mamedova Maria Klimova Latchezar Avramov Oxana Semyachkina-Glushkovskaya Saratov State University 83 Astrakhanskaya Str.Saratov 410012Russia Institute of Electronics Bulgarian Academy of Sciences Tsarigradsko Chaussee Blvd.72Sofia 1784Bulgaria

The detection of early gastric cancer that often develops asymptomatically is crucial for improving patient *** photodynamic diagnosis(PDD)of gastric cancer using 5-aminolevulinic acid/protoporphyrin IX(5-ALA/PpIX)has been reported in several ***,the selectivity of PDD of gastric tumor is poor with often false-positive results that require the development of new methods to improve PDD for early gastric ***,a measure of the complexity of gastric microcirculation(multi-scale entropy,MSE)and the detrendedfluctuation analysis(DFA)were applied as additional tools to detect early gastric cancer in *** this experimental study,we used our original model of metastatic adenocarcinoma in the stomach of a *** induce a gastric tumor,we used a long-term combination(for 9 months,which is 1/2 of the life of rats)of two natural factors,such as chronic stress(overpopulation being typical for modern cities)and the daily presence of nitrites in food and drinks,which are common ingredients added to processed meat andfish to help preserve *** results clearly show that both methods,namely,PDD using 5-ALA/PpIX and complexity/correlation analysis,can detect early gastric cancer,which was confirmed by histological ***-cancerous areas in the stomach were detected as an intermediatefluorescent signal or MSE level between normal and malignant lesions of the ***,in some cases,PDD with 5-ALA/PpIX produced a false-positivefluorescence of exogenousfluorophores due to its accumulation in benign and inflammatory areas of the *** fact indicates that the PDD itself is not sufficient for the correct diagnosis of gastric cancer,and the use of additional characteristics,e.g.,complexity measures or scaling exponents,can significantly improve the diagnostic accuracy of PDD of gastric cancer that should be confirmed in further clinical studies and applications.

关键词： Experimental model of gastric cancer in animals highly heterogeneous adenocarcinoma photodynamic diagnosis complexity measures gastric microcirculation

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The Effectiveness of a Portable Fluorescence Spectrophotometer for Early Detection of Oral Cancer

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Open Journal of Stomatology 2022年第1期12卷 30-31页

作者： Katsuhiro Asaka Akira Fukatsu Chiaki Komine Laboratory Medicine for Dentistry Graduate School of Dentistry at Matsudo Nihon University School of Dentistry at Matsudo Matsudo Japan Department of Laboratory Medicine and Dentistry for the Compromised Patient Nihon University School of Dentistry at Matsudo Matsudo Japan

Purpose: To investigate whether photodynamic diagnosis (PDD) using a portable fluorescence spectrophotometer (FC-1) can easily and objectively discriminate between normal and tumor cells at the dental chairside, and to further compare it with PDD that requires speculum examination by focusing on protoporphyrin IX (PPIX). Methods: Three cell lines (2 human oral squamous cell carcinoma-derived cell lines, HSC-2 and HSC-3 cells, and oral keratinocytes, HOK cells) were cultured. 5-Aminolevulinic acid hydrochloride (5-ALA) and deferoxamine mesylate (DFO) were mixed in DMEM, and the mixture was set to Control (DMEM only) and PDD (5-ALA+DFO) groups. And then, a fluorescence was measured under room temperature (RT) and 37°C (Incubation) by using FC-1. In this study, the two conditions were combined with the Control and PDD groups to form the Control/RT, Control/Incubate, PDD/RT, and PDD/Incubate groups. Additionally, the amount of singlet oxygen (1O2) generated by irradiation with 405 nm LED was measured using electron spin resonance spectroscopy to detect PPIX in the cell supernatant after 24 hours. Results: In HSC-2 and HSC-3, the fluorescence intensity values increased significantly at 2 hours between the Control/RT and PDD/RT groups. In addition, there was a significant difference between HSC-2 and HSC-3 compared to HOK. In all cell lines, the fluorescence intensity values of the PDD/Incubate group were significantly higher than those of the PDD/Control group. The amount of 1O2 generated by 405 nm LED irradiation was higher in the cell supernatants of all cell lines in the order of Control/RT Conclusion: It is suggested that PDD using FC-1 can clearly distinguish between normal cells and tumor cells in vitro studies using cell lines at 2 hours und

关键词： photodynamic diagnosis Portable Fluorescence Spectrophotometer Oral Cancer Protoporphyrin IX

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