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Effects of pH on the peripheral light-harvesting antenna complex for Rhodopseudomonas palustris

Science China(Life Sciences) 2008年第8期51卷 760-766页

作者： FENG Juan, LI XueFeng & LIU Yan School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, China School of Life Science and Technology University of Electronic Science and Technology of China Chengdu China

In this work steady-state absorption spectroscopy, circular dichroism spectroscopy and sub-micro-second time-resolved absorption spectroscopy were used to investigate the effect of pH on the struc-tures and functions of LH2 complex for Rhodopseudomonas palustris. The results revealed that: (1) B800 Bchla was gradually transformed to free pigments absorbing around 760 nm on the minutes timescale upon the induction of strong acidic pH, and subsequently there disappeared the CD signal for Qy band of B800 in the absence of B800. In addition, Carotenoids changed with the similar tendency to B850 BChl. (2) The introduction of strong basic pH gave rise to no significant changes for B800 Bchla, while B850 BChla experienced remarkable spectral blue-shift from 852 to 837 nm. Similar phe-nomenon was seen for the CD signal for Qy band of B850. Carotenoids displayed strong and pH-independent CD signals in the visible range. (3) In the case of both physiological and basic pH, broad and asymmetrical positive Tn←T1 transient absorption appeared following the pulsed photo-excitation of Car at 532 nm. By contrast, the featureless and weak positive signal was observed on the sub-microsecond timescale in the acidic pH environment. The aforementioned experimental results indicated that acidic pH-induced removal of B800 Bchla prevented the generation of the caro-tenoid triplet state (3Car*), which is known to be essential for the photo-protection function. Neverthe-less, carotenoids can still perform this important physiological role under the basic pH condition, where the spectral blue shift of B850 exerts little effect on the overall structure of the cyclic aggregate, therefore favoring the formation of carotenoid triplet state.

关键词： pH-induced B800-removal triplet state photo-protection function

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Research Progress of Sunscreen Agents and Encapsulate Technology

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China Detergent & Cosmetics 2023年第2期8卷 56-61页

作者： Zheng Xiaoyang Meng Xiangjing Li Min Liu Bo Zhang Xiangkui Duan Chong gang Zhang Daizhou Ling Peixue Wang Dongliang Shandong Engineering Research Center of Skin and Mucosa Drug Administration Shandong Academy of Pharmaceutical SciencesChina Hebei Edible Bird’s Nest Fresh Stew Technology Innovation Center China

The harm of ultraviolet radiation to human skin and the mechanism of traditional sunscreen agents and natural sunscreen agents were *** agents were usually encapsulated with delivery systems due to the instability and skin *** research progress of several common sunscreen delivery systems was introduced,the future research direction of sunscreen agents was also prospected.

关键词： UV radiation sunscreen encapsulate photo-protection

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