检索结果-南通市图书馆

Tumor suppressor function of ezrin-radixin-moesin-binding phosphoprotein-50 through β-catenin/E-cadher in pathway in human hepatocellular cancer

引用

World Journal of Gastroenterology 2013年第8期19卷 1306-1313页

作者： Xiu-Lan Peng Meng-Yao Ji Zi-Rong Yang Jia Song Wei-Guo Dong Department of Gastroenterology Renmin Hospital of Wuhan University

AIM:To determine the effect and molecular mechanism of ezrin-radixin-moesin-binding phosphoprotein-50(EBP50) in hepatocellular carcinoma(HCC).METHODS:Three human HCC cell lines,i.e.,SMMC7721,HepG2 and Hep3B,were *** transfected the Pbk-CMV-HA-EBP50 plasmid into SMMC7721 cells with Lipofectamine 2000 to overexpress *** blotting were performed to determine the effects of the plasmid on EBP50 expression and to detect the expression of β-catenin and E-cadherin before and after the transfection of the plasmid into SMMC7721 *** vitro cell proliferation was assessed with a Cell Counting Kit-8(CCK-8) *** cycle distribution was assessed with flow *** and migration ability of before and after the transfection were determined with a transwell *** apoptosis was demonstrated with Annexin *** effect of EBP50 overexpressing on tumor growth in vivo was performed with a xenograft tumor model in nude ***:The transfection efficiency was confirmed with Western blotting(1.36 ± 0.07 vs 0.81 ± 0.09,P 50 was significantly lower than control cells(P 50(61.3% ± 3.1% vs 54.0% ± 2.4%,P 50 compared with control cells(5.8 ± 0.8 vs 21.6 ± 1.3,P 50 compared with control cells(14.8% ± 2.7% vs 3.4% ± 1.3%,P 50 compared with control cells(0.28 ± 0.07 vs 0.56 ± 0.12,P 50 could obviously slow the growth of HCC derived from SMM

关键词： Hepatocellular carcinoma Ezrin-radixinmoesin-binding phosphoprotein-50 Growth Migration Invasion

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：