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Biological Analysis and Response to Low phosphate Stress of phosphate transporter Family 1 (PHT1) genes in Solanum tuberosum L.

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Phyton-International Journal of Experimental Botany 2023年第8期92卷 2325-2348页

作者： Yangyang Du Yuanyuan Bao Lu Zheng Qinglong Tian Xinyong Zhang Key Laboratory of Crop Germplasm Innovation and Sustainable Utilization Collage of Agronomy and BiotechnologyYunnan Agricultural UniversityKunming650201China Laboratory of Fruit and Vegetable Preservation and Processing College of Food Science and TechnologyYunnan Agricultural UniversityKunming650201China

Inorganic phosphate(Pi)is one of the main nutrients necessary for plant *** transporters mediate the acquisition,transport and recycling of phosphate,which is essential for plant growth and *** PHT1 has been reported in many plants at home and abroad,it is rarely studied in ***,it is of great signiﬁcance to study the PHT1 family members in order to understand the molecular response mechanism of potato in low phosphate *** this study,a total of 6 potato PHT1 genes were identiﬁed and *** was found that after the expression of different members of potato PHT1 gene,there were certain differences in amino acids and proteins,and the transmembrane domains ranged from 6 to *** difference in the secondary and tertiary protein structure of potato PHT1 also led to a difference in protein *** addition,the expression of the PHT1 gene in potato increased obviously during 3~9 h of Pi deﬁciency ***,the expression levels of different genes in roots,stems and leaves are distinctly different,but the expression levels of the StPHT1;6 and StPHT1;10 genes are very high in roots,stems and leaves,indicating that these two genes may participate in the absorption of Pi in potato and play a role in Pi *** two genes play a major role in the regulation of expression under short-term Pi deﬁciency *** results provide an important reference for further understanding the evolution and function of potato phosphate transporters,and have important signiﬁcance for improving the ability of potato to tolerate low Pi.

关键词： Potato phosphate transporter gene short-term phosphate-deficiency stress biological analysis

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