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Glutamine synthetase as an early marker for hepatocellular carcinoma based on proteomic analysis of resected smal hepatocel ular carcinomas

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Hepatobiliary & Pancreatic Diseases International 2010年第3期9卷 296-305页

作者： Jiang Long,Zhen-Wei Lang,Hua-Guang Wang,Tai-Ling Wang,Bao-En Wang and Si-Qi Liu Liver Research Center,Beijing Friendship Hospital, Capital Medical University,Beijing 100050,China Department of Hepatology,Department of Pathology, Beijing Youan Hospital,Capital Medical University,Beijing 100069,China Department of Pharmaceutical Affairs,Beijing Chaoyang Hospital,Capital Medical University,Beijing 100020,China Department of Pathology,China-Japan Friendship Hospital,Beijing 100029,China Beijing Genomics Institute,Chinese Academy of Science,Beijing Airport Industrial Zone B-6,Shunyi,Beijing 101300,China Liver Research Center Beijing Friendship Hospital Capital Medical University Beijing China.

BACKGROUND:Hepatocellular carcinoma(HCC)is a highly malignant tumor with a poor *** small HCCs possess most of the characteristics of early HCC,we investigated small HCCs to screen potential biomarkers for early ***:proteins were extracted from 10 sets of paired tissue samples from HBV-infected small-HCC *** extracted proteins were well resolved by two-dimensional *** HCC-associated proteins were then identified by MALDI-TOF/TOF MS following image *** blotting and immunohistochemistry were used to assess glutamine synthetase(GS)and phenazine biosynthesislike domain-containing protein(PBLD)expression in liver ***-linked immunosorbent assays in 152 serum samples(from 49 healthy donors,24 patients with liver cirrhosis,and 79 with HCC)were used to further assess the significance of GS ***:Fifteen up-regulated and three down-regulated proteins were *** blotting confirmed GS overexpression and decreased PBLD expression in liver *** showed that GS was expressed in 70.0%(84/120)of HCCs and 35.8%(43/120)of nontumor tissues;PBLD was expressed in 74.2%(89/120) of nontumor tissues and 40.8%(49/120)of *** Chi-square test showed significant expression differences between HCCs and adjacent *** with this,serum GS levels in HCC patients were significantly higher than those in liver cirrhosis patients and healthy donors,while the latter two groups were also significantly *** addition, a diagnostic cutoff value of 2.6 mg/ml was used for GS;it was elevated in 19(76.0%)of 25 HCC patients with AFP≤20 ng/ml and 47(88.7%)of 53 HCC patients with AFP≤200 ng/***:GS and PBLD are abnormally expressed in most *** may be a novel serum marker for early HCC, especially for those patients with low AFP levels(≤200 ng/ml).

关键词： glutamine synthetase phenazine biosynthesis-like domain-containing protein proteomics

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