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Influence of sinomenine on protein profiles of peripheral blood mononuclear cells from ankylosing spondylitis patients： a pharmacoproteomics study

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Chinese Medical Journal 2013年第19期126卷 3645-3650页

作者： HUANG Zhi-xiang TAN Jin-hui LI Tian-wang DENG Wei-ming QIU Ke-wei LIAO Ze-tao ZENG Zhao-qiu Department of Rheumatology and Immunology Guangdong No. 2Provincial People's Hospital Guangzhou Guangdong 510317 China Department of Rheumatology Third Affiliated Hospital of Sun Yat-sen University Guangzhou Guangdong 510630 China

Background Ankylosing spondylitis （AS） is a common inflammatory rheumatic disease which lacks satisfactory treatment so *** （SIN） is an alkaloid and has recently been utilized in treating multiple rheumatic diseases including AS in China,but its exact mechanism remains to be *** study investigated the alteration of proteome in peripheral blood mononuclear cells （PBMCs） from AS *** Thirty AS patients were enrolled in this *** from each AS patient were cultured in medium with or without SIN *** PBMCs proteins from both groups were separated by two-dimensional electrophoresis （2-DE） and analyzed by mass spectrometry （MS）.Two differentially expressed proteins were then chosen to be verified using Western *** Seven proteins,including α-synuclein （SNCA）,calmodulin （CALM）,acidic leucine-rich nuclear phosphoprotein 32 family member A （ANP32A）,chloride intracellular channel protein 1 （CLIC1）,guanine nucleotide-binding protein G（I）/G（S）/ G（T） subunit beta-1 （GNB1）,gelsolin （GSN） and histone H2B type 1-M （HISTH2BM）were over-expressed,while coronin1A （CORO1A） was under-expressed in the SIN-treated *** bioinformatics search indicated that the changes of SNCA,ANP32A and CLIC1 pertained to apoptosis,while changes of GSN and CORO1A were associated with both apoptosis and inhibition of immunological *** GSN and CORO1A were selected to validate by Western blotting and the results were consistent with those of *** There were 8 differentially expressed proteins in the SIN-treated PBMCs,which might shed some light on the mechanism of SIN in the treatment of AS.

关键词： ankylosing spondylitis sinomenine peripheral blood mononuclear cells pharmacoproteomics

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