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Development of Marker-Free Transgenic Cry1Ab Rice with Lepidopteran pest resistance by Agrobacterium Mixture-Mediated Co-transformation

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Rice science 2009年第3期16卷 181-186页

作者： QI Yong-bin YE Sheng-hai Lu Yan-ting JIN Qing-sheng ZHANG Xiao-ming Institute of Crop and Nuclear Technology Utilization Zhejiang Academy of Agricultural Sciences Hangzhou 310021 China Biotechnology Institute Zhejiang University Hangzhou 310029 China

CrylAb gene was transformed into four rice varieties, Zhejing 22, Zhejing 27, Jiahua 1 and Xiushui 63 mediated by Agrobacterium-mixture co-transformation. Rice genotype had an important effect on callus induction and transformation efficiency. Different mixtures of Agrobacterium strains （EHA105 and EHA101） contained Hpt and CrylAb genes resulted in different frequencies of resistant calli. There was no correlation between the frequency of transformants with the ratio of the Agrobacterium strain mixture contained Hpt and CrylAb genes. A total of 509 transgenic plants were obtained from the four rice varieties, and 272 T2 progenies were analyzed for CrylAb and Hpt genes. PCR analysis revealed that 412 regenerated plants were Hpt positive （80.94%）, 62 plants were also CrylAb co-transformants （15.05% in total frequency）, and 42 plants among the 272 T2 progenies were CrylAb positive but Hpt negative. This suggests that marker-free transgenic plants could be produced by co-transformation mediated by mixed Agrobacterium strains with the selectable marker gene and target gene Southern blot analysis of five independent marker-free T2 transgenic lines co-transformed from Zhejing 22 showed that CrylAb gene had been inserted into rice genome with a single copy. The transgenic plants showed significantly stronger resistance to lepidopteron than the non-transgenic plants under no application of insecticides against lepidopteron.

关键词： rice (Oryza sativa) Agrobacterium mixture-mediated co-transformation CrylAb gene Hpt gene gene transfer marker-free transgenic plants pest resistance

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Comparison of three transgenic Bt rice lines for insecticidal protein expression and resistance against a target pest, Chilo suppressalis （Lepidoptera： Crambidae）

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Insect Science 2016年第1期23卷 78-87页

作者： Ya-Nan Wang Kai-Qie Ke Yun-He Li Lan-Zhi Han Yan-Min Liu Hong-Xia Hua Yu-Fa Peng State Key Laboratory for Biology of Plant Disease and Insect pests Institute of Plant Protection Chinese Academy of Agricultural ScienceBeUing 100193 College of Plant Science & Technology Huazhong Agricultural University Wuhan 430070 China

Two transgenic rice lines （T2A-1 and T1C-19b） expressing cry2A and crylC genes, respectively, were developed in China, targeting lepidopteran pests including Chilo suppressalis （Walker）（Lepidoptera： Crambidae）. The seasonal expression of Cry proteins in different tissues of the rice lines and their resistance to C. suppressalis were assessed in comparison to a Bt rice line expressing a crylAb/Ac fusion gene, Huahui 1, which has been granted a biosafety certificate. In general, levels of Cry proteins were T2A- 1 〉 Huahui I 〉 T1C-19b among rice lines, and leaf 〉 stern 〉 root among rice tissues. The expression patterns of Cry protein in the rice line plants were similar： higher level at early stages than at later stages with an exception that high Cry 1C level in T 1 C- 19b stems at the maturing stage. The bioassay results revealed that the three transgenic rice lines exhibited significantly high resistance against C. suppressalis larvae throughout the rice growing season. According to Cry protein levels in rice tissues, the raw and corrected mortalities of C. suppressalis caused by each Bt rice line were the highest in the seedling and declined through the j ointing stage with an exception for T1 C-19b providing an excellent performance at the maturing stage. By comparison, T1C-19b exhibited more stable and greater resistance to C. suppressalis larvae than T2A-1, being close to Huahui 1. The results suggest crylC is an ideal Bt gene for plant transformation for lepidopteran pest control, and T1C-19b is a promising Bt rice line for commercial use for tolerating lepidopteran rice pests.

关键词： Chilo suppressalis crylAb/Ac cryl C ery2A pest resistance transgenic rice

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An SI integrated pest management model with pesticide resistance to susceptible pests

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International Journal of Biomathematics 2020年第5期13卷 125-147页

作者： Jinyang Li Qingdao Huang Bing Liu School of Mathematics Jilin University Changchun 130012P.R.China School of Mathematics and Information Science Anshan Normal University Anshan 114007 P.R.China

In this paper,epidemnic diseases among pests are assumed to occur,so pests are divided into susceptible pests and infected pests,and only susceptible pests are harmful to *** spraying pesticides and releasing of natural enermies and infected pests to control pests,as well as the long-term application of the same pesticide to induce resistance,an integrated pest management with pesticide resistance is *** pollution emission model is introduced to model the action process of pesticides,which well reflects its residual and delay *** using comparison theorem of impulsive differential equation and analysis method,the threshold condition for eradication of susceptible pests is *** we analyze the frequency of spraying pesticide on the success of pests *** shows that it is not that the more frequently pesticides are applied,the better the result of the susceptible pests control *** the sensitivity analysis,the key factors on the threshold are ***,the strategies to control susceptible pests are given,including switching pesticides and releasing infected pests and natural enemies elastically.

关键词： pest management model eradication of susceptible pests global attractive-ness pest resistance pest control strategies

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Physiological Response and resistance of Plants to Disease and pest Stress

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Plant Diseases and pests 2024年第4期15卷 1-6,12页

作者： Cong CHEN Xiaoxia CAI Yimin PENG Lingling LIU Zilei LIANG Guangzu DU Yanjun GUO Institute of Pomology/College of Life Sciences Zhaoqing UniversityZhaoqing 526061China College of Plant Protection Yunnan Agricultural University/State Key Laboratory for Conservation and Utilization of Bio-Resources in YunnanKunming 650201China

This paper outlines the physiological responses of plants to pathogenic microbial infection and pest feeding stress,as well as the resistance characteristics of plants to diseases and pests,and proposes new directions for future research on crop resistance to diseases and *** objective of this paper is to provide a reference framework for the breeding of crops with enhanced resistance to diseases and pests,the utilization of natural immunity in crops,and the efficient prevention and control of diseases and *** framework is intended to facilitate the healthy and sustainable development of the agricultural industry.

关键词： Pathogenic microorganism pest Disease resistance pest resistance resistance breeding Defensive response

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Relationship between trophic component of different poplar strains and occurrence of Saperda poplnea

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Journal of Forestry Research 2001年第4期12卷 263-265,278页

作者：高长启孙守慧任晓光宋丽文张晓军戴万琢肖辉庄翠云易德国朱振宇任海波吉林省林业科学研究院长春130031 松原地区防治站 131200 乾安防治站 131400

An experimental area of poplar was established in Songyuan of Jilin Province in 1999 for testing the resistance of different poplar stains to Saperda poplnea. Incidence of S. poplnea on ten poplar strains were investigated, and the main trophic component of branches of these poplar trees were measured and analyzed in April 2001. The results showed that there existed significant difference in population size of S. poplnea on different poplar strains, and the branches of these poplar strains have significant difference in nutrient component and content of amino acids. The population size of this pest had a significantly positive correlation with dissolvable total sugar and water content but had no significant correlation with content of total nitrogen and protein nitrogen.

关键词： Saperda poplnea Poplar strains pest resistance

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