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Re-evaluation of physical interaction between plant peroxisomes and other organelles using live-cell imaging techniques

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Journal of Integrative Plant Biology 2019年第7期61卷 836-852页

作者： Kazusato Oikawa Makoto Hayashi Yasuko Hayashi Mikio Nishimura Biomacromolecules Research Team RIKEN Center for Sustainable Resource Science2-1 HirosawaWakoSaitama 351-0198Japan Department of Bioscience Nagahama Institute of Bio-Scie nee and Technology1266 Tamura-ChoNagahama 526-0829Japan Department of Biology Faculty of scienceNiigata UniversityNiigata 950-2181Japan Department of Cell Biology National Institute for Basic BiologyOkazaki 444-8585Japan

The dynamic behavior of organelles is essential for plant survival under various environmental conditions. Plant organelles, with various functions,migrate along actin filaments and contact other types of organelles, leading to physical interactions at a specific site called the membrane contact site. Recent studies have revealed the importance of physical interactions in maintaining efficient metabolite flow between organelles.In this review, we first summarize peroxisome function under different environmental conditions and growth stages to understand organelle interactions. We then discuss current knowledge regarding the interactions between peroxisome and other organelles, i.e., the oil bodies, chloroplast, and mitochondria from the perspective of metabolic and physiological regulation, with reference to various organelle interactions and techniques for estimating organelle interactions occurring in plant cells.

关键词： physical interaction plant peroxisomes live-cell

Plant peroxisomes at the crossroad of NO and H2O2 metabolism

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Journal of Integrative Plant Biology 2019年第7期61卷 803-816页

作者： Francisco J.Corpas Luis A.del Río José M.Palma Group of Antioxidants Free Radicals and Nitric Oxide in Biotechnology Food and Agriculture Department of Biochemistry Cell and Molecular Biology of Plants Estación Experimental del Zaidín CSIC

Plant peroxisomes are subcellular compartments involved in many biochemical pathways during the life cycle of a plant but also in the mechanism of response against adverse environmental conditions.These organelles have an active nitro-oxidative metabolism under physiological conditions but this could be exacerbated under stress situations.Furthermore,peroxisomes have the capacity to proliferateand also undergo biochemical adaptations depending on the surrounding cellular status.An important characteristic of peroxisomes is that they have a dynamic metabolism of reactive nitrogen and oxygen species(RNS and ROS)which generates two key molecules,nitric oxide(NO)and hydrogen peroxide(H2O2).These molecules can exert signaling functions by means of post-translational modifications that affect the functionality of target molecules like proteins,peptides or fatty acids.This review provides an overview of the endogenous metabolism of ROS and RNS in peroxisomes with special emphasis on polyamine and uric acid metabolism as well as the possibility that these organelles could be a source of signal molecules involved in the functional interconnection with other subcellular compartments.

关键词： Plant peroxisomes NO H2O2 metabolism

Plant peroxisomes:Small organelles with versatility and complexity

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Journal of Integrative Plant Biology 2019年第7期61卷 782-783页

作者： Jianping Hu MSU-DOE Plant Research Laboratory and Plant Biology Department Michigan State University

peroxisomes are small,highly dynamic,and multifunctional organelles in eukaryotes.Essential to plant survival,peroxisomes house various crucial metabolic activities,such as degradation of hydrogen peroxide(H2O2),lipid metabolism,photorespiration,and hormone biosynthesis and catabolism,and remodel their proteome in response to developmental and environmental changes(Hu et al.2012;Pan and Hu 2018).The four reviews and three research articles in this special issue on plant peroxisomes provide new insights into the diverse roles and dynamics of these structurally simple but functionally complicated organelles,raising exciting new questions for future investigations.

关键词： Plant peroxisomes Small organelles versatility complexity

peroxisomes,oxidative stress,and inflammation

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World Journal of Biological Chemistry 2012年第5期3卷 93-97页

作者： Stanley R Terlecky Laura J Terlecky Courtney R Giordano Department of Pharmacology Wayne State University School of Medicine540 E.Canfield Ave.DetroitMI 48201United States

peroxisomes are intracellular organelles mediating a wide variety of biosynthetic and biodegradative reactions.Included among these are the metabolism of hydrogen peroxide and other reactive species,molecules whose levels help define the oxidative state of cells.Loss of oxidative equilibrium in cells of tissues and organs potentiates inflammatory responses which can ultimately trigger human disease.The goal of this article is to review evidence for connections between peroxisome function,oxidative stress,and inflammation in the context of human health and degenerative disease.Dysregulated points in this nexus are identified and potential remedial approaches are presented.

关键词： peroxisomes Oxidative stress Inflammation

Arabidopsis RING Peroxins are E3 Ubiquitin Ligases that Interact with Two Homologous Ubiquitin Receptor Proteins

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Journal of Integrative Plant Biology 2013年第1期55卷 108-120页

作者： Navneet Kaur Qingzhen Zhao Qi Xie Jianping Hu MSU-DOE Plant Research Laboratory Michigan State University Institute of Genetics and Developmental Biology the Chinese Academy of Sciences

peroxisomes are essential eukaryotic organelles that mediate various metabolic processes. Peroxisome import depends on a group of peroxisome biogenesis factors called peroxins, many of which are evolutionarily conserved. PEX2, PEX10, and PEX12 are three RING-finger-domain-containing integral membrane peroxins crucial for protein import. In yeast （Saccharomyces cerevisae）, RING peroxins act as E3 ligases, facilitating the recycling of the peroxisome import receptor protein PEX5 through ubiquitination. In plants, RING peroxins are essential to plant vitality. To elucidate the mode of action of the plant RING peroxins, we employed in vitro assays to show that the Arabidopsis RING peroxins also have E3 ligase activities. We also identified a PEX2-interacting protein, DSK2b, which is a member of the ubiquitin receptor family known to function as shuttle factors ferrying polyubiquitinated substrates to the proteasome for degradation. DSK2b and its tandem duplicate DSK2a are localized in the cytosol and the nucleus, and both interact with the RING domain of PEX2 and PEX12. DSK2 artificial microRNA lines did not display obvious defects in plant growth or peroxisomal processes, indicating functional redundancies among Arabidopsis ubiquitin receptor proteins. Our results suggest that Arabidopsis RING peroxins can function as E3 ligases and act together with the ubiquitin receptor protein DSK2 in the peroxisomal membrane-associated protein degradation system.

关键词： Arabidopsis RING domain proteins peroxisomes ubiquitin receptor.

Interactomic study on interaction between lipid droplets and mitochondria

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Protein & Cell 2011年第6期2卷 487-496页

作者： Jing Pu Cheol Woong Ha Shuyan Zhang Jong Pil Jung Won-Ki Huh Pingsheng Liu National Laboratory of Biomacromolecules Institute of BiophysicsChinese Academy of SciencesBeijing 100101China Graduate University of Chinese Academy of Sciences Beijing 100101China School of Biological Sciences Research Center for Functional CellulomicsInstitute of MicrobiologySeoul National UniversitySeoul 151-747Republic of Korea

An increasing body of evidence shows that the lipid droplet,a neutral lipid storage organelle,plays a role in lipid metabolism and energy homeostasis through its interaction with mitochondria.However,the cellular functions and molecular mechanisms of the interaction remain ambiguous.Here we present data from transmission electron microscopy,fluorescence imaging,and reconstitution assays,demonstrating that lipid droplets physically contact mitochondria in vivo and in vitro.Using a bimolecular fluorescence complementation assay in Saccharomyces cerevisiae,we generated an interactomic map of protein-protein contacts of lipid droplets with mitochondria and peroxisomes.The lipid droplet proteins Erg6 and Pet10 were found to be involved in 75%of the interactions detected.Interestingly,interactions between 3 pairs of lipid metabolic enzymes were detected.Collectively,these data demonstrate that lipid droplets make physical contacts with mitochondria and peroxisomes,and reveal specific molecular interactions that suggest active participation of lipid droplets in lipid metabolism in yeast.

关键词： peroxisomes bimolecular fluorescence complementation assay protein-protein interaction lipid metabolism Erg6

At Long Last： Evidence for Pexophagy in Plants

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Molecular Plant 2014年第8期7卷 1257-1260页

作者： Tamar Avin-Wittenberg Alisdair R. Fernie Max-Planck-Institute of Molecular Plant Physiology Am Mühlenberg 1 14476 Potsdam-Golm Germany

peroxisomes are highly dynamic single-membrane-bound eukaryotic organelles displaying great variability in enzymatic content （Platta and Erdmann, 2007）. Plant peroxisomes function in a plethora of crucial development and stress ameliorating processes. Among the functions of plant peroxisomes are 13-oxidation of fatty acids, phytohormone production, participation in photorespiration, the glyoxyalte cycle, detoxification processes, and signal molecule generation （Hu et al., 2012）.

关键词： AUTOPHAGY peroxisomes ARABIDOPSIS DEGRADATION