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Opening of liver mitochondrial permeability transition pore in streptozotocin-induced diabetic rats and its inhibition by methanol fraction of Ficus mucoso(Welw) root bark

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Journal of Integrative Medicine 2019年第6期17卷 446-454页

作者： Olubukola Titilope Oyebode Foluso Olayemi Ogunbiyi Olufunso Olabode Olorunsogo Laboratories for Biomembrane Research and Biotechnology Department of Biochemistry Faculty of Basic Medical Sciences College of Medicine University of Ibadan

Objective: Several pathologies arise from the inappropriate opening of the mitochondrial permeability transition(mPT) pore. In this regard, inhibition of mPT pore represents a cytoprotective approach to preserve mitochondrial function for treatment of diseases characterized by excessive tissue wastage such as diabetes mellitus. The aim of this study, therefore, was to study the effects of fractions of Ficus mucoso, a medicinal plant used in the traditional treatment of diabetes, on mPT pore in normal and streptozotocin(STZ)-induced diabetic rat ***: Different solvent fractions of the crude methanol extract of F. mucoso were obtained by vacuum liquid chromatography and were tested on the mPT pore. Of all the fractions tested, methanol fraction of F. mucoso(MFFM) was the most potent and was used for in vivo studies. Diabetes mellitus was induced by a single intraperitoneal injection of 60 mg/kg STZ, while treatment lasted for 14 d. In vivo, 30 male Wistar rats were divided into five groups: A, normo-glycemic control(distilled water);B, STZ(65 mg/kg;diabetic control);C, STZ + MFFM(25 mg/kg);D, STZ + MFFM(50 mg/kg);E, STZ + glibenclamide(5 mg/kg). The mPT, mitochondrial ATPase activity, lipid peroxidation and cytochrome c release were assessed spectrophotometrically while blood glucose levels were monitored using ***: In vitro, the solvent fractions of F. mucoso, at all concentrations tested, had no effect on the mPT pore, in the absence of calcium, with no significant release of cytochrome c. Interestingly, calciumdependent pore opening was inhibited by all solvent fractions of F. mucoso, with the MFFM having the highest inhibitory effect of 83% at 3 mg/mL. Induction of opening of the mPT pore, significant(P < 0.001) enhancement of mitochondrial ATPase activity and elevated malondialdehyde(MDA) levels in STZ-induced diabetes were significantly(P < 0.001) reversed by MFFM and were comparable with the effects of glibenclamide, a standard antidiab

关键词： Mitochondria Ficus mucoso permeability transition Cytochrome c

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Schisandrin B decreases the sensitivity of mitochondria to calcium ioninduced permeability transition and protects against ischemia-reperfusion injury in rat hearts

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Acta Pharmacologica Sinica 2007年第10期28卷 1559-1565页

作者： Po-yee CHIU Hoi-yan LEUNG Ada HL SIU Michel KTPOON Kam-ming KO Department of Biochemistry Hong Kong University of Science and Technology Hong Kong SAR China

Aim:In order to elucidate the molecular mechanism underlying the cardioprotectionafforded by schisandrin B （Sch B）,the effect of Sch B treatment on the sensitivityof mitochondria to Ca2+-stimulated permeability transition （PT） was investigatedin rat hearts under normal and ischemia-reperfusion （I-R） ***:Myocardial I-R injury caused an increase in the sensitivity of mitochondria toCa2+-stimulated PT in *** enhanced sensitivity to mitochondrial PT wasassociated with increases in mitochondrial Ca2+content as well as the extent ofreactive oxidant species production in vitro and cytochrome c release in *** cardioprotection afforded by Sch B pretreatment against I-R-induced injurywas paralleled by the decrease in the sensitivity of myocardial mitochondria toCa2+-stimulated PT,particularly under I-R ***:The resultssuggest that Sch B treatment increases the resistance of myocardial mitochondriato Ca2+-stimulated PT and protects against I-R-induced tissue injury.

关键词： schisandrin B mitochondria permeability transition calcium myocardial ischemiareperfusion

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