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Superiority of Laparoscopy in the peritoneal dialysis catheter Reset Surgery

Journal of Huazhong University of Science and Technology(Medical Sciences) 2015年第1期35卷 71-75页

作者：陈文莉丁国华郑直刘昌璇 Department of Nephrology Renmin Hospital of Wuhan University Department of Hepatopancreatobiliary Surgery Wuhan Central Hospital Tongji Medical College Huazhong University of Science and Technology Department of Nephrology Wuhan Central Hospital Tongji Medical College Huazhong University of Science and Technology

peritoneal dialysis catheter surgery has been used in clinical treatment for nearly 40 years, and open surgery under local anesthesia is the conventional method. However, catheter displacement after open surgery is still the thorny issue during our clinical practice. Then the reset surgery is often required to be taken again. Nowadays, laparoscopic peritoneal dialysis catheter draws our attention due to its advantages of accurate positioning, smaller incision, and less pain, and its clinical application has been limited. While laparoscopic surgery is recognized, there are few relevant studies on whether there is difference during the catheter reset process between the two surgical approaches. In this study, we mainly discussed the rate of secondary catheter migration, the incidence of complications after catheter reset for two surgical approaches and the hospital stay as well as the total clinical cost for the two surgical approaches. In this study, we retrospectively analyzed 25 cases of end-stage renal disease, who received catheterization for peritoneal dialysis and regular peritoneal dialysis in our hospital from March 2010 to December 2013, and had a medical history of catheter migration. We collected the relevant clinical data for all patients. Fifteen patients selected laparoscopic catheter reset, and 10 patients selected the traditional surgical method for catheter reset by themselves. For all patients enrolled, we analyzed the incidence of secondary catheter migration and postoperative complications, hospitalization time, and total cost for different methods of reset. Through the studies above, we found that laparoscopic peritoneal dialysis catheter surgery offered accurate catheter location and a small incision that was easy to heal. Besides, the incidence of postoperative complications for the laparoscopic surgery was lower than that for traditional surgical approach for catheter reset. The average hospitalization time for laparoscopic surgery was shorter than that for the traditional surgical approach

关键词： laparoscope peritoneal dialysis catheter catheter migration

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The effect of matrine against biofilm formation in Escherichia coli

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广西医科大学学报 2017年第6期34卷 801-805页

作者： Yuming Huang Xi Peng Yizheng Li He Zhao Ju Qin Yashan Song Qiaoyuan Wu Yunhua Liao Renal Division Department of MedicalThe First Affiliated Hospital of Guangxi Medical University Department of Nephrology The Affiliated Hospital of Guilin Medical College

Objective:To investigate the effect of matrine against biofilm formation of Escherichia coli(E.coli)in the early stage.Methods:Biofilms of E.coli(ATCC25922)was induced by using a peritoneal dialysis catheter in 96-well plates.After treatment with matrine for 24 h,the formation of biofilm on the catheters was observed by scanning electron microscope.The expressions of the flagellar gene(fliC)and motility genes(motA and motB)were determined by qPCR,and the protein expression of fliC was detected by western blotting.Results:The biofilm formation of E.coli was suppressed by different concentrations(less than the minimal inhibitory concentration)of matrine,and the mRNA levels of motA and motB as well as the mRNA and protein expression levels of fliC were decreased in a dose-dependent manner.Conclusion:Matrine could inhibit biofilm formation of E.coli via downregulating the expression of motA,motB and fliC.

关键词： matrine Escherichia coli peritoneal dialysis catheter biofilm

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