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Spatiotemporal microRNA profile in peripheral nerve regeneration:miR-138 targets vimentin and inhibits Schwann cell migration and proliferation

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Neural Regeneration Research 2018年第7期13卷 1253-1262页

作者： Travis B.Sullivan Litchfield C.Robert Patrick A.Teebagy Shannon E.Morgan Evan W.Beatty Bryan J.Cicuto Peter K.Nowd Kimberly M.Rieger-Christ David J.Bryan Department of Translational Research Lahey Hospital & Medical Center Tissue Engineering Laboratory Lahey Hospital & Medical Center Department of Plastic and Reconstructive Surgery Lahey Hospital & Medical Center

While the peripheral nervous system has regenerative ability,restoration of sufficient function remains a *** has been shown to be localized in axonal growth fronts and associated with nerve regeneration,including myelination,neuroplasticity,kinase signaling in nerve axoplasm,and cell migration;however,the mechanisms regulating its expression within Schwann cell（SC） remain *** aim of this study was to profile the spatial and temporal expression profile of micro RNA（mi RNA） in a regenerating rat sciatic nerve after transection,and explore the potential role of mi R-138-5 p targeting vimentin in SC proliferation and migration.A rat sciatic nerve transection model,utilizing a polyethylene nerve guide,was used to investigate mi RNA expression at 7,14,30,60,and 90 days during nerve *** levels of mi RNA expression were determined using microarray analysis and subsequently validated with quantitative real-time polymerase chain *** vitro assays were conducted with cultured Schwann cells transfected with mi RNA mimics and assessed for migratory and proliferative *** top seven dysregulated mi RNAs reported in this study have been implicated in cell migration elsewhere,and GO and KEGG analyses predicted activities essential to wound *** of one of these,mi RNA-138-5 p,into SCs reduced cell migration and *** R-138-5 p has been shown to directly target vimentin in cancer cells,and the luciferase assay performed here in rat Schwann cells confirmed *** results detail a role of mi R-138-5 p in rat peripheral nerve regeneration and expand on reports of it as an important regulator in the peripheral nervous system.

关键词： non-coding RNA neural regeneration nerve guide sciatic nerve transection peripheral nerve injury wound healing gene ontology processes Kyoto Encyclopedia of genes and Genomes pathways microarray luciferase assay

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