检索结果-南通市图书馆

Irisin-loaded electrospun core-shell nanofibers as calvarial periosteum accelerate vascularized bone regeneration by activating the mitochondrial SIRT3 pathway

引用

Regenerative Biomaterials 2024年第1期3卷 44-57页

作者： Xi Hua Mingzhuang Hou Lei Deng Nanning Lv Yong Xu Xuesong Zhu Huilin Yang Qin Shi Hao Liu Fan He Department of Orthopaedics The First Affiliated Hospital of Soochow UniversitySoochow UniversitySuzhou 215006China Orthopaedic Institute Suzhou Medical CollegeSoochow UniversitySuzhou 215000China Department of Orthopedics Suzhou Wuzhong People’s HospitalSuzhouJiangsu Province 215128China Department of Orthopedic Surgery Lianyungang Clinical College of Xuzhou Medical UniversityLianyungang 222003China

The scarcity of native periosteum poses a significant clinical barrier in the repair of critical-sized bone defects.The challenge of enhancing regenerative potential in bone healing is further compounded by oxidative stress at the fracture site.However,the introduction of artificial periosteum has demonstrated its ability to promote bone regeneration through the provision of appropriate mechanical support and controlled release of proosteogenic factors.In this study,a poly(L-lactic acid)(PLLA)/hyaluronic acid(HA)-based nanofibrous membrane was fabricated using the coaxial electrospinning technique.The incorporation of irisin into the core-shell structure of PLLA/HA nanofibers(PLLA/HA@Irisin)achieved its sustained release.In vitro experiments demonstrated that the PLLA/HA@Irisin membranes exhibited favorable biocompatibility.The osteogenic differentiation of bone marrow mesenchymal stem cells(BMMSCs)was improved by PLLA/HA@Irisin,as evidenced by a significant increase in alkaline phosphatase activity and matrix mineralization.Mechanistically,PLLA/HA@Irisin significantly enhanced the mitochondrial function of BMMSCs via the activation of the sirtuin 3 antioxidant pathway.To assess the therapeutic effectiveness,PLLA/HA@Irisin membranes were implanted in situ into critical-sized calvarial defects in rats.The results at 4 and 8 weeks post-surgery indicated that the implantation of PLLA/HA@Irisin exhibited superior efficacy in promoting vascularized bone formation,as demonstrated by the enhancement of bone matrix synthesis and the development of new blood vessels.The results of our study indicate that the electrospun PLLA/HA@Irisin nanofibers possess characteristics of a biomimetic periosteum,showing potential for effectively treating critical-sized bone defects by improving the mitochondrial function and maintaining redox homeostasis of BMMSCs.

关键词： periosteum irisin mitochondrial function critical-sized bone defect redox homeostasis

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Current practice in autologous rib and costal-cartilage harvest for rhinoplasty: A systematic review

引用

Chinese Journal of Plastic and Reconstructive Surgery 2024年第1期6卷 41-48页

作者： Mengyuan Jiang Haizhong Huo Lu Zhang 1.Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine 2.Department of General Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine Department of Plastic and Reconstructive Surgery Shanghai Ninth People's Hospital Shanghai Jiao Tong University School of Medicine Department of General Surgery Shanghai Ninth People's Hospital Shanghai Jiao Tong University School of Medicine

Background: Autologous costal grafts are used universally in clinical practice for rhinoplasty and reconstruction.However, surgeons worldwide have not agreed on the details of graft harvesting, including rib selection, side preference, operation mode, cutting methods, and handling of the periosteum and perichondrium. This study aimed to provide an overview of the novel techniques used for auto-rib harvesting in rhinoplasty within the past 5years and identify potential avenues for future research.Methods: We searched for related articles in Pub Med, Embase, and Web of Science from 2019 to 2023, summarized crucial but controversial steps in recent practice, and analyzed their theoretical basis and clinical feasibility.Results: Auto-rib and cartilage open harvest is still mainstream in rhinoplasty and reconstruction, with the 5th to8th ribs and cartilage being the most used. The laparoscopic harvest is gaining attention, being second only to the open harvest, with the 9th/10th ribs and cartilages being particularly convenient. The clinical applications of fullcut and split-cut methods differ in their advantages. Except for some special reasons, almost all studies tended to preserve the periosteum and perichondrium in situ, and few surgeons chose to harvest the grafts on the left side.Conclusion: Multiple techniques have emerged, requiring surgeons to balance the benefits and risks of various strategies at each step. New theories and techniques should be fully tested promptly and in clinical practice before wide application. Overall, a professional consensus is needed for better directivity, precision, and stability in clinical practice.

关键词： Rhinoplasty Autologous graft Cartilage Rib periosteum Perichondrium

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Giant nodular fasciitis originating from the humeral periosteum:A case report

引用

World Journal of Clinical Cases 2022年第5期10卷 1572-1579页

作者： Shi-Li Yu Ping-Li Sun Jian Li Meng Jia Hong-Wen Gao Department of Pathology The Second Hospital of Jilin UniversityChangchun 130041Jilin ProvinceChina

BACKGROUND Nodular fasciitis(NF)is a self-limiting tumor that mostly occurs in the subcutaneous superficial fascia.NF originating from the appendicular periosteum is extremely rare.A large NF lesion of periosteal origin can be misdiagnosed as a malignant bone tumor and may cause overtreatment.CASE SUMMARY A right axillary mass was found in a 46-year-old man and was initially diagnosed intraoperatively as low-grade sarcoma,but later diagnosed as NF after postresection histopathological evaluation.Furthermore,fluorescence in situ hybridization analysis revealed a USP6 gene rearrangement that confirmed the diagnosis.To the best of our knowledge,this is the first case of NF in the humeral periosteum.CONCLUSION NF poses a diagnostic challenge as it is often mistaken for sarcoma.Postoperative histopathological examination of whole sections can be combined with immunohistochemical staining and,if necessary,the diagnosis can be confirmed by molecular detection,and thus help avoid overtreatment.

关键词： Nodular fasciitis periosteum Differential diagnosis USP6 Fluorescence in situ hybridization Case report

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Metal-phenolic networks modified polyurethane as periosteum for bone regeneration

引用

Chinese Chemical Letters 2022年第3期33卷 1623-1626页

作者： Qingyi Zhang Kai Huang Jie Tan Xiongxin Lei Liping Huang Yuting Song Qianjin Li Chenyu Zou Huiqi Xie Laboratory of Stem Cell and Tissue Engineering Orthopedic Research InstituteMed-X Center for MaterialsState Key Laboratory of BiotherapyWest China HospitalSichuan UniversityChengdu 610041China

Treatment of bone defects still poses a great challenge in orthopedic clinics, and the vital role of periosteum in such processes has attracted widespread attention. However, studies focusing on the oxidative stress micro-environment with an artificial periosteum at the site of defect have been scarce. The intrinsic anti-oxidative properties and therapeutic potential for bone defects of metal-phenolic networks(MPNs)have provided a potential solution to this. Herein, we have developed a protocatechualdehyde + zinc ion(PCA+Zn^(Ⅱ)) MPN coating on a thermoplastic polyurethane membrane with a one-pot method to fabricate a new-type of periosteum with meritorious biocompatibility and abilities of modulating oxidative stress condition and promoting osteogenesis and mineralization for better bone regeneration, which has shown to be a promising strategy for constructing artificial periosteum with various MPNs.

关键词： Metal-phenolic networks Surface modification periosteum Oxidative stress Mineralization

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Reconstruction of segmental bone defects in the rabbit ulna using periosteum encapsulated mesenchymal stem cells-loaded poly（lactic-co-glycolic acid） scaffolds

引用

Chinese Medical Journal 2012年第22期125卷 4031-4036页

作者： ZHANG Xin QI Yi-ying ZHAO Teng-fei LI Dan DAI Xue-song NIU Lie HE Rong-xin Zhejiang Univ Sch Med Affiliated Hosp 2 Dept Orthoped Surg Hangzhou 310009 Zhejiang Peoples R China Zhejiang Univ Dept Polymer Sci & Engn Minist Educ Key Lab Macromol Synth & Functionalizat Hangzhou 310027 Zhejiang Peoples R China Dongping Cty Peoples Hosp Dept Orthoped Surg Tai An 271500 Shandong Peoples R China

Background Repair of large bone defects remains a challenge for clinicians. The present study investigated the ability of mesenchymal stem cells （MSCs） and/or periosteum-loaded poly（lactic-co-glycolic acid）（PLGA） to promote new bone formation within rabbit ulnar segmental bone defects. Methods Rabbit bone marrow-derived MSCs （passage 3） were seeded onto porous PLGA scaffolds. Forty segmental bone defects, each 15 mm in length, were created in the rabbit ulna, from which periosteum was obtained. Bone defects were treated with either PLGA alone （group A）, PLGA ＋ MSCs （group B）, periosteum-wrapped PLGA （group C） or periosteum-wrapped PLGA/MSCs （group D）. At 6 and 12 weeks post-surgery, samples were detected by gross observation, radiological examination （X-ray and micro-CT） and histological analyses. Results Group D, comprising both periosteum and MSCs, showed better bone quality, higher X-ray scores and a greater amount of bone volume compared with the other three groups at each time point （P 〈0.05）. No significant differences in radiological scores and amount of bone volume were found between groups B and C （P 〉0.05）, both of which were significantly higher than group A （P 〈0.05）. Conclusions Implanted MSCs combined with periosteum have a synergistic effect on segmental bone regeneration and that periosteum plays a critical role in the process. Fabrication of angiogenic and osteogenic cellular constructs or tissue-engineered periosteum will have broad applications in bone tissue engineering.

关键词： mesenchymal stem cell periosteum bone defect regeneration

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Effect of estrogen on gene expression of fatty acid synthase in periosteum

引用

Chinese Medical Journal 2009年第15期122卷 1775-1779页

作者： ZHENG Rui-min LIN Shou-qing LIU Yong HUANG Man-ting GONG Wei-yan WU Zhi-hong Department of Obstetrics and Gynecology Peking Union Medical College Hospital Chinese Academy of Medical Sciences & Peking Union Medical College Beijing 100730 China Department of Orthopaedics Peking Union Medical College Hospital Chinese Academy of Medical Sciences & Peking Union Medical College Beijing 100730 China

Background Estrogen deficiency contributes to postmenopausal osteoporosis. periosteum might be a potential target of estrogen, but the underlying mechanism at gene level is far from being elucidated. The objective of this study was to investigate the correlation between estrogen and fatty acid synthase （FAS） expression in periosteum. Methods Human periosteum cells were cultured in vitro. Expressed genes in the substrated cDNA library were verified using semi-quantitative PCR and real-time PCR. The expression of FAS in periosteum of ovarectomized （OVX） SD rats was investigated. Results FAS gene was most significantly expressed in the subtracted cDNA library of periosteal cells screened by semi-quantitative PCR. Low FAS expression was verified by real-time PCR in the estrogen exposed human periosteum rather than in the control. The estradiol levels were （20.81±12.62） pg/ml, （19.64±4.35） pg/ml and （13.47±1.84） pg/ml in the sham group, the control, and the OVX group, respectively. The estradiol levels in the OVX group was significantly lower （P=-0.0386）. The FAS gene expression in periosteum in the OVX group, sham group, and control group was 3.09±1.97, 1.33±0.47 and 1.51±1.32, respectively. The gene expression in the OVX group was significantly higher （P=0.0372）. Conclusion Estrogen modulates FAS gene expression in in vitro human perisoteum as well as in in vivo rat periosteum.

关键词： estradiol genetic research fatty acid synthase complex osteoporosis periosteum

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

periosteum derived stem cells for regenerative medicine proposals: boosting current knowledge

引用

World Journal of Stem Cells 2014年第3期6卷 266-277页

作者： Concetta Ferretti Monica Mattioli-Belmonte Department of Clinical and Molecular Sciences School of Medicine Università Politecnica delle Marche

periosteum is a thin fibrous layer that covers most bones. It resides in a dynamic mechanically loaded environment and provides a niche for pluripotent cells and a source for molecular factors that modulate cell behaviour. Elucidating periosteum regenerative poten-tial has become a hot topic in orthopaedics. This review discusses the state of the art of osteochondral tissue engineering rested on periosteum derived progenitor cells(PDPCs) and suggests upcoming research direc-tions. Periosteal cells isolation, characterization and migration in the site of injury, as well as their differen-tiation, are analysed. Moreover, the role of cell mecha-nosensing and its contribution to matrix organization, bone microarchitecture and bone stenght is examined. In this regard the role of periostin and its upregulation under mechanical stress in order to preserve PDPC sur-vival and bone tissue integrity is contemplated. The re-view also summarized the role of the periosteum in the field of dentistry and maxillofacial reconstruction. The involvement of microRNAs in osteoblast differentiation and in endogenous tissue repair is explored as well. Fi-nally the novel concept of a guided bone regenerationbased on the use of periosteum itself as a smart mate-rial and the realization of constructs able to mimic the extracellular matrix features is talked out. Additionally, since periosteum can differentiate into insulin produc-ing cells it could be a suitable source in allogenic trans-plantations. That innovative applications would takeadvantage from investigations aimed to assess PDPCimmune privilege.

关键词： periosteum Mesenchymal stem cells Mi-croRNA Bone tissue engineering Bone turn-over

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Reconstruction of Hemipelvectomy Defect Using a Fillet Flap with Femoral periosteum

引用

Surgical Science 2010年第2期1卷 49-52页

作者： Masaki Yazawa Tsuyoshi Kaneko Sumitoshi Katsumata Kazuo Kishi 不详

Backgrounds: Reconstruction after hemipelvectomy is very important for rehabilitation into society. The pelvis plays an important role for support of the intra-abdominal organs. Methods: We operated 3 cases using fillet flaps with the femoral periosteum for reconstruction of hemipelvectomy defect. Results: It is useful to elevate the flap all around with the femoral periosteum, because the periosteum can be sutured to supporting pelvic structures with the aim to support intra-abdominal organs. Conclusion: Without alternative supports for the bony pelvis, pelvic reconstructions are at risk for hernia and it may be difficult for outpatients to fit their habiliments after radical cure. The rigid support for the intra-abdominal organs occurs in association with the suture pelvic and femoral periosteum.

关键词： Hemipelvectomy Reconstruction Fillet Flap periosteum

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Experiment study on combined free periosteum grafting and new reconstituted bone xenograft in repairing segmental defects

引用

Journal of Medical Colleges of PLA(China) 2000年第4期15卷 283-287页

作者：袁志马平胡蕴玉罗卓荆金格勒吕荣王军

Objective: To study the efficacy of combined use of a new grafting material, new reconstituted bone xenograft (NRBX) and free periosteal graft in repair of segmental bony defects. Methods: NRBX was made by combining recombi- nant human BMP2(rhBMP2) and an antigen-free bovine cancellous bone (BCB) as a carrier. NRBX was used alone, in combination with free periosteal graft to repair a 1. 5 cm defect in the radius of rabbit. The defect-repairing capability for each of the treatment modalities was assessed with radiographical, biomechanical, densitometrical and histological methods. Results: NRBX used alone was capable of healing the defect in large by 16 weeks, with a similar repair process and mecha- nism seen with econstituted bone xenograft (RBX). Combined use of NRBX and free periosteal graft was superior in terms of increased and quality of the new bone formed at the early stage of the repair pass (within 12 weeks) to NRBX used alone, with the defect basically healed by 12 weeks. Conclusion: Both methods are effective in repairing segmental bone defects, but NRBX used in combination with free periosteal graft is preferred, because of the satisfactory osteogenesis, osteoconduction and osteoinduction.

关键词： periosteum bone transplantation transplantation, heterologous

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Determining the critical size of a rabbit rib segmental bone defect model

引用

Regenerative Biomaterials 2016年第5期3卷 323-328页

作者： Fengzhen Liu Kun Chen Lei Hou Keyi Li Dawei Wang Bin Zhang Xiumei Wang Liaocheng People’s Hospital Medical College of Liaocheng UniversityLiaocheng 252000P.R.China Tsinghua University Beijing 100084P.R.China

In order to establish and standardize the rabbit rib segmental bone defect model,it is of vital importance to determine rabbit rib critical size defect(CSD).According to the general time needed for spontaneous long-bone regeneration,three-month observation period was set to determine the CSD.The rabbit rib segmental bone defects with different sizes from 1 to 5cm with or without periosteum were performed in the eighth rib of 4-month-old male New Zealand rabbits and underwent Xray examinations at the 4th,8th and 12th weeks postoperatively.The gross and histological examinations at postoperative week 12 were evaluated,which showed that the critical sizes in the rabbit rib models with and without periosteum were 5 and 2 cm,respectively.This study provides prerequisite data for establishing rabbit rib CSD model and evaluating bonematerials using this model.

关键词： animal test bone critical size defect rib defect periosteum

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：