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MiR-214-3p Prevents the Development of perioperative neurocognitive disorders in Elderly Rats

Current Medical Science 2022年第4期42卷 871-884页

作者： Yu-hao WANG Yong-wang CHEN Wan-li XIAO Xue-lian LI Lan FENG Yu-lin LIU Xiao-xia DUAN Department of Anesthesiology the Affiliated Hospital of Southwest Medical UniversityLuzhou 646000China Laboratory of Anesthesiology Southwest Medical UniversityLuzhou 646000China Department of Anesthesiology Southwest Medical UniversityLuzhou 646000China

Objective:This study aimed to identify microRNAs(miRNAs)involved in the development of perioperative neurocognitive disorders(PND).Methods:Plasma exosomal miRNA expression was examined in patients before and after cardiopulmonary bypass(CPB)using microarray and qRT-PCR and these patients were diagnosed as PND later.Elderly rats were subjected to CPB,and the cognitive functions were examined.Bioinformatics analysis was conducted to predict the targets of miR-214-3p.Rats were administered rno-miR-214-3p agomir before or after CPB to investigate the role of miR-214-3p in PND development.Results:We identified 76 differentially expressed plasma exosomal miRNAs in PND patients after surgery(P0.58),including the upregulated hsa-miR-214-3p(P=0.002399392).Prostaglandin-endoperoxide synthase 2(PTGS2)was predicted as a miR-214-3p target.In rats,CPB reduced the platform crossing numbers and target quadrant stay time,accompanied with hippocampal neuronal necrosis.The rno-miR-214-3p level was significantly increased in plasma exosomes but decreased in rat hippocampus after surgery,exhibiting a negative correlation(P<0.001,r=-0.762).A negative correlation between miR-214-3p and PTGS2 protein expression was also observed in the hippocampus after surgery.Importantly,rno-miR-214-3p agomir treatment,before or after surgery,significantly increased the platform crossing numbers(P=0.035)and target quadrant stay time(P=0.029)compared with negative control.Hippocampal PTGS2 protein level was increased in the untreated surgery group and decreased in response to rno-miR-214-3p agomir treatment before or after surgery(both P<0.05 vs.negative control).Conclusion:These data suggest that miR-214-3p/PTGS2 signaling contributes to the development of PND,serving as a potential therapeutic target for PND.

关键词： perioperative neurocognitive disorder sexosome hippocampus miR-214-3p prostaglandin-endoperoxide synthase 2

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Impact of edaravone on serum CXC chemokine ligand-13 levels and perioperative neurocognitive disorders in elderly patients with hip replacement

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Chinese Medical Journal 2021年第13期134卷 1610-1615页

作者： Hai-Hui Xie Hong-Yan Ma Shu Zhang Jian-Wen Li Qi Han Hui-Qun Chen Bo-Qin Su Jian-Ping Zhou Department of Anesthesiology Affiliated Dongguan People’s Hospital of Southern Medical UniversityDongguanGuangdong 523018China Department of Nephrology First Hospital of Jilin UniversityChangchunJilin 130021China Department of Orthopaedics Affiliated Dongguan People’s Hospital of Southern Medical UniversityDongguanGuangdong 523018China

Background:perioperative neurocognitive disorders(PND)are a series of severe complications in the perioperative and anesthetic periods with a decline in memory,execution ability,and information processing speed as the primary clinical manifestation.This study aimed to evaluate the impact of edaravone(EDA)on PND and peripheral blood C-X-C motif chemokine ligand 13(CXCL13)levels in elderly patients with hip replacement.Methods:A total of 160 elderly patients undergoing hip arthroplasty in Affiliated Dongguan People’s Hospital of Southern Medical University(from March 2016 to March 2018)were randomly and double-blindly categorized into an EDA group and a control group(CON).Group EDA was administered intravenously EDA 30 min before surgery,and group CON was administered intravenously saline.The cognitive function of the two groups was evaluated 1-day before the operation and at 1 and 12 months after surgery,and the incidence of post-operative delirium was tested on days 1,3,and 7 after surgery using the Chinese version of the confusion assessment method.Serum CXCL13 and interleukin(IL)-6 concentrations were measured before anesthesia,during surgery(30 min after skin incision),and on days 1,3,and 7 after surgery.The continuous variables in accordance with normal distribution were tested using the Student’s t test,the continuous variables without normal distribution using the Mann-Whitney U test,and categorical variables by the x 2 test or Fisher exact test.Results:The incidence of post-operative delirium within 7 days after surgery was significantly higher in group CON than that in group EDA(31.3%vs.15.0%,t=-5.6,Pneurocognitive dysfunc

关键词： Edaravone perioperative neurocognitive disorder Chemokine CXC ligand 13 Interleukin-6

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