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2014浙江省医学会医学遗传学学术年会

作者： Yuqin Luo Chenming Xu Yixi Sun Liya Wang Songchang Chen Fan Jin Department of Reproductive Genetics Women's HospitalZhejiang University School of Medicine Key Laboratory of Reproductive Genetics(Zhejiang University) Ministry of Education Centre of Reproductive Medicine Women's HospitalZhejiang University School of Medicine

pericentric inversion can produce recombinant gametes,meiotic segregation studies on the relation between the frequency of recombinants and the inverted segment size are ***-color fluorescence in-situ hybridization(FISH)was performed to analyze the meiotic behavior in five inv(l)carriers with different *** gametes were absent in patient 1,whereas the percentages of the recombinants in patients 2,3,4 and 5 were of 9.2%,15.3%,17.3%and 40.9%,respectively.A significant difference was present for the frequencies of the recombinant spermatozoa among the five patients(P0.05).The meiotic segregation of nine inv(l)carriers(including ours)is now available.A significant correlation was discovered between the rate of recombination and the proportion of the chromosome implicated in the inversion(R=0.9435,P<0.001).The frequency of the recombinant gametes was directly related to the proportion of the chromosome that was ***-FISH allowed an additional comprehension of the patterns of meiotic segregation and provided accurate genetic counseling.

关键词： meiotic segregation pericentric inversion sperm FISH chromosome 1 infertility

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Species identification of crested gibbons(Nomascus) in captivity in China using karyotyping-and PCR-based approaches

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Zoological Research 2018年第5期39卷 356-363页

作者： Wen-Hui Nie Jin-Huan Wang Wei-Ting Su Yu Hu Shui-Wang He Xue-Long Jiang Kai He Kunming Institute of Zoology Chinese Academy of Sciences Kyoto University Museum Kyoto University

Gibbons and siamangs （Hylobatidae） are well-known for their rapid chromosomal evolution,which has resulted in high speciation rate within the *** the other hand,distinct karyotypes do not prevent speciation,allowing interbreeding between individuals in captivity,and the unwanted hybrids are ethically problematic as all gibbon species are endangered or critically ***,accurate species identification is crucial for captive breeding,particularly in China where studbooks are *** based on external morphology is difficult,especially for hybrids,because species are usually similar in *** this study,we employed G-banding karyotyping and fluorescence in situ hybridization （FISH） as well as a PCR-based approach to examine karyotypic characteristics and identify crested gibbons of the genus Nomascus from zoos and nature reserves in *** characterized and identified five karyotypes from 21 individuals of *** karyotypes and mitochondrial and nuclear genes,we identified three purebred species and three hybrids,including one F2 hybrid between *** and *** results also supported that *** and *** shared the same inversion on chromosome 7,which resolves arguments from previous *** results demonstrated that both karyotyping and DNA-based approaches were suitable for identifying purebred species,though neither was ideal for hybrid *** advantages and disadvantages of both approaches are *** results further highlight the importance of animal ethics and welfare,which are critical for endangered species in captivity.

关键词： F2 hybrid gibbon Fluorescence insitu hybridization Nomascus pericentric inversion Species identification Animal welfare

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Cytogenetic analysis in 61 couples with spontaneous abortions

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Chinese Medical Journal 2001年第2期114卷 88-89页

作者：江静傅曼芬王德芬 China 200025

Objective　To examine the relationship between spontaneous abortion and chromosomal ***　Couples who had one or more consecutive spontaneous abortions and had normal genitals were enrolled for cytogenetic karyotype analysis. Results　In the 61 couples, the detected incidence was 11.5%, with five Robertsonian translocations, one reciprocal translocation, and one pericentric inversion of chromosome 7. Conclusion　Chromosomal abnormalities may play an important role in fetal wastage.

关键词： spontaneous abortion chromosomal abnormality reciprocal translocation pericentric inversion

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