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Disease-tolerance of transgenic tobacco plants expressing Ah-AMP gene of Amaranthus hypochondriacus

Progress in Natural Science:Materials International 2003年第5期 44-48页

作者： CHEN Yan, GUO Hongman, JIANG Ying, LI Changqing, ZHAO Wenming and TIAN Yingchuan ( 1 . Life Science and Technology Institute of Xi'an Jiaotong University, Xi'an 710049, China 2. Institute of Microbiology, Chinese A caciemy of Sciences, Beijing 100080, China) A graduate student from Northeast Agricultural University, China Life Science and Technology Institute of Xi'an Jiaotong University，Xi'an 710049，China Institute of Microbiology，Chinese Academy of Sciences，Beijing 100080，China Institute of Microbiology，Chinese Academy of Sciences，Beijing 100080，China A graduate student from Northeast Agricultural University，China Life Science and Technology Institute of Xi'an Jiaotong University，Xi'an 710049，China

An antimicrobial peptide gene from Amaranthus hypochondriacus, Ah-AMP , was amplified by PCR and cloned. Sequence analysis results revealed that this gene is 261 bp in length encoding a precursor polypeptide of 87 ammo acid residues. Ah-AMP gene was inserted in the binary vector pBin438 to construct a plant expression vector pBinAH916. Leave explants of Nicotiana tabacum var. SRI were transformed with Agrobacterium tumefaciens LBA4404 harboring the above expression vector. Results from PCR, Southern and Northern blot analyses confirmed that the Ah-AMP gene had been integrated into the tobacco genome and was transcribed at mR-NA level. Two bacterial-resistant.transgenic plants were selected by inoculating the plants with Pseudomonas solanacearum and statistic analysis of two T1 lines showed that the resistance increased by 2. 24 and 1. 62 grade and the disease index decreased by 49. 6% and 37. 3% respectively when compared with the non-transformed control plants SRI. The results from challenging the plan

关键词： Amaranthus hypochondriacus antimicrobial peptide Ah-AMP gene transgenic tobacco pathogen resistant.

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