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Establishment of paternity testing system using microsatellite markers in Chinese Holstein

Journal of Genetics and Genomics 2008年第5期35卷 279-284页

作者： Fei Tian Dongxiao Sun Yuan Zhang College of Animal Science and Technology and Key Laboratory of Animal Genetics and Breeding of Ministry of Agriculture China Agricultural University Beijing 100094 China

To estimate the efficiency of microsatellite markers in paternity testing among Chinese Holstein, 30 microsatellite loci were used to differentiate 330 Chinese Holstein genotypes, according to the calculation of the allele frequency, number of alleles, effective number of alleles, genetic heterozygosity, polymorphic information content （PIC）, and the exclusion probability in this cattle population. The results demonstrated that the exclusion probability ranged from 0.620 in locus BM1818 to 0.265 in locus INRA005 with the average of 0.472 and 11 microsatellite markers exceeding 0.5. The combined exclusion probability of nine microsatellite markers was over 0.99. The result showed that paternity testing of Chinese Holstein was basically resolved using the nine microsatellite markers selected.

关键词： Chinese Holstein rnicrosatellite paternity testing exclusion probability

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paternity testing of Selected Abaca （Musa textilis L. Nee） Hybrids Using Morphometric Markers

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Journal of Agricultural Science and Technology 2010年第5期4卷 101-105页

作者： F. C. L. Zapico C. H. M. Aguilar J. M. Aujero B. Y. Disca Science Department College of Natural Sciences and Mathematics Mindanao State University Fatima General Santos City 9500The Philippines

Investigation into the paternity of four abaca （Musa textilis L, Nee） hybrids was done to ascertain the mode of transmission of selected morpho-agronomic traits and to detect possible heterosis. In situ morphological characterization was undertaken using twenty five qualitative and six quantitative characters. Results revealed that a great majority of the qualitative traits were shared by both parents and their hybrids. For the rest, the qualitative traits were inherited from one or the other parent though some variant phenotypes （i.e. chimerism） were also noted in the hybrids. Cases ofheterosis were also observed and this could be exploited to increase fiber yield in the hybrids. Though inconclusive due to factors such as the heterogenous nature of abaca plants in the field and the susceptibility of morphological traits to environmental fluctuations, this study has provided baseline information on abaca hybridity that can be verified using more robust technologies as molecular markers.

关键词： Abaca (Musa textilis L. Nee) paternity testing morphological characterization heterosis.

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STR mutation and tri-allelic phenomena in one paternity testing case

STR mutation and tri-allelic phenomena in one paternity test...

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第八次全国医学遗传学学术会议(中华医学会2009年医学遗传学年会)

作者：王琳马旭 Genetic Center of National Research Institute for Family Planning The Graduate School of Pecking Union Medical School Beijing

Objective To report a case of paternity identification,which result cover STR mutation and tri-allelic special genetic *** & Results 24 STR were genotyped by PCR-STR *** the 15 STR,which are D3S1358,TH01, D21S11,D18S51,D5S818,D13S317,D7S820,D16S539,CSF1PO,VWA,D8S1179,TPOX,FGA,Penta E and Penta D,the obligate paternal alleles in the child all have corresponding alleles in the alleged father except for *** D8S1179genotype of alleged father,alleged mother and the child are 10/14,12,12/*** we scan the other 9 STR loci,which are D18S1364, D12S391,D13S325,D6S1043,D2S1772,D11S2368,D22-GATA,D8S1132 and *** the obligate paternal alleles in the child have corresponding alleles in the alleged *** the locus D18S1364,we found the alleged father and the child are all tri-al-lelic. It seemed that the alleged father had transport his duo-allelic 14/18 to the *** also perform single detection of D18S1364, same result have been *** three band of the locus are uneven,we call it type 1 *** the genotype result and calculate the combined paternity index(CPI).The CPI are far more above *** we can say the tested Man can not be excluded as the biological father of the child in *** dispute phenomena in D8S1179 can be thought as STR *** obligate paternal allele 15 may be come from her father’s *** & Conclusions The tri-allelic is a common result when person has an extra chromosome or small chromosome *** mutation is a common genetic phenomena,the mutation rate are 1‰on average. This is our first time find STR mutation and tri-allelic in one *** we searched in the Medline,There is no related report.

关键词： STR mutation tri-allelic paternity testing

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Investigation on the genetic-inconsistent paternity cases using the MiSeq FGx system

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Forensic Sciences Research 2022年第4期7卷 702-707页

作者： Anqi Chen Ruiyang Tao Chengtao Li Suhua Zhang Shanghai Key Laboratory of Forensic Medicine Shanghai Forensic Service PlatformAcademy of Forensic ScienceMinistry of JusticeShanghaiChina Department of Forensic Medicine School of Basic Medical SciencesShanghai Medical CollegeFudan UniversityShanghaiChina

Mutations might challenge the paternity index calculation in forensic *** many studies have focussed on the autosomal short tandem repeats(A-STR),the mutation status of sex chromosomes and single nucleotide polymorphism(SNP)remain *** generation sequencing(NGS),known as high throughput and large sequence polymorphism,is a promising tool for forensic *** describe the mutation landscapes in the paternity cases with genetic inconsistencies,a total of 63 parentage confirmed paternity cases contained at least one mismatched locus have been *** mutations were subsequently evaluated using Verogen’s MPSForenSeqTM DNASignature Kit and a microsatellite instability(MSI)detection *** result showed 98.41%(62/63)of the cases had no additional autosomal mutations even when the number of A-STRs increased to *** for the sex chromosomes,about 11.11%(7/63)of the cases exhibited either X-STR or Y-STR mutations.D2S1338,FGAand Penta Ewere the most frequent altered STRs,which suggested they might be the mutation *** addition,a male with sex chromosome abnormality was observed accidently,whose genotype might be 47,XXY,rather than *** 56.90%of the STR loci possessed isoalleles,which might result in higher STR *** Mendelian incompatibility was detected among the SNP markers,which indicated that SNP was a more reliable genetic marker in the genetic-inconsistent paternity cases.

关键词： Forensic sciences forensic genetics paternity testing next generation sequencing(NGS) ForenSeqTM DNASignature Prep Kit

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Mutation Analysis of STR Locus on 23 Autosomes in Hainan Population

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Forensic Medicine and Anatomy Research 2022年第1期10卷 1-6页

作者： Weihua Xu Nie Yao Xiaojuan Li Zhichao Ma Hongtao Zhou Shengmiao Fu Xinping Chen Hainan Provincial Key Laboratory of Cell and Molecular Genetic Translational Medicine Central Laboratory Hainan General Hospital Hainan Hospital Affiliated to the Hainan Medical College Haikou China Verification Centre of Forensic Medicine Hainan General Hospital Hainan Hospital Affiliated to the Hainan Medical College Haikou China

[Objective] To analyze the mutation signature and regularity of STR locus on 23 autosomes in paternity testing cases in Hainan. [Methods] A total of 2715 paternity testing cases accepted by the Forensic Medical Identification Centre of our hospital from 2017 to 2020 derived from counties and cities in Hainan Province were collected, the cases containing gene mutations were selected, the mutation rate and details of each locus were counted, and the mutation regu-larity of 23 STR loci was analyzed. [Results] Of the 2715 cases identified as “support”, 1487 were triplet cases and 1640 were dyad cases, totaling 4614 meioses;There were 50 gene mutation events (including 17 triplet mutations and 33 dyad mutations), with an average mutation rate of 0.0047% and a cumulative mutation rate of 1.0837%. A total of 19 of the 23 STR loci were mutated, with a mutation rate of 0.1301% at the D12S391 locus and 0.0217% at five loci, TPOX, D1S1656, D2S441, D22S1045, and PentaD, while no muta-tion events were found at four loci, D19S433, TH01, D13S317, and D7S820. Of the 50 mutation events, 47 were one-step mutations, 1 was two-step, and 2 were three-step. There were 35 paternal mutations (13 triplets and 22 dyads), 6 maternal mutations (4 triplets and 2 dyads), and 9 indeterminate pater-nal/maternal mutations, with a paternal to maternal mutation ratio of 5.83:1. [Conclusion] The mutation rate of D12S391 locus is the highest, and the muta-tion rate of TPOX, D1S1656, D2S441, D22S1045 and PentaD loci is the lowest in Hainan population, and paternal mutations are more than maternal muta-tions. In the paternity test, if 1 - 3 STR loci do not conform to the genetic law, especially when the mutant locus is homozygous or the next of kin is identi-fied, it is necessary to use other kits to review and increase the number of loci or use the second-generation sequencing technology to confirm, carefully de-termine the mutation and ensure the accuracy of the identification conclusion.

关键词： STR Locus paternity testing Mutation

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