检索结果-南通市图书馆

检索条件"主题词=pancreatic cancer cells"

共 1 条记录，以下是1-10 订阅

全选清除本页清除全部题录导出标记到"检索档案"

详细简洁

排序：

Preparation and in vitro antitumor activities of triptolide and cyclopamine loaded polymeric micelles

Preparation and in vitro antitumor activities of triptolide ...

引用

2018年第十二届中国药物制剂大会

作者：刘德俊 Di Zhang Yixue Pei Yongjie Liu Lingyun Xu School of Biology and Pharmaceutical Engineering Wuhan Polytechnic University

Objective: In order to overcome the poor solubility of triptolide(TP) and cyclopamine(CPA), enhance their anticancer effects, and decrease their side effects, triptolide and cyclopamine encapsulated polymeric micelle system(TP/CPA-PM) was prepared and its in-vitro antitumor activities were investigated. Methods: Self-prepared PEG-PLC was used as a carrier material to prepare a novel polymeric micelle system containing both TP and CPA(TP/CPA-PM;TP:CPA = 1:1, w/w) using acetone solvent evaporation method. The micelles were characterized in terms of particle size, analyzed by laser particle size analyzer, entrapment efficiency, determined by high-performance liquid chromatography(HPLC), and in-vitro drug release, determined by combining dialysis method with HPLC. The MTS assay was performed at different time points to determine the inhibitory effect of TP/CPA-PM and free-TP/CPA with different concentrations on the proliferation of three kinds of human pancreatic carcinoma cell lines, namely Miapace-2 cells, Kras cells and HPSC cells. Results: The average particle size of TP/CPA-PM(TP:CPA = 1:1, w/w) was(54.3±2.6) nm(n = 3), and the encapsulation efficiency of TP and CPA was(48.7±7.7%)(n = 3) and(58.9±6.0%)(n = 3), respectively. The drug release curve showed that TP in TP/CPA-PM demonstrated fast-release profile, while CPA in TP/CPAPM indicated sustained-release profile. The release of CPA from micelles was faster at pH 5.2 than at pH 7.4. After 24 h of incubation at 37 °C, 39.0% of CPA was released at pH 7.4, while 58.4% was released at pH 5.2. Compared with free-TP/CPA, TP/CPA-PM significantly inhibited the proliferation of the three kinds of pancreatic cancer cells in a dose-dependent and time-dependent manner. Conclusion: These findings indicate that the combination of triptolid and cyclopamine loaded in polymeric micelles is an effective strategy to treat pancreatic tumors.

关键词： Triptolide cyclopamine polymer micelles encapsulation efficiency in-vitro release profile pancreatic cancer cells

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

全选清除本页清除全部题录导出标记到“检索档案”

共1页 << < 1 > >>

回到顶部

执行限定条件

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：