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Effects of notoginosides on proliferation and upregulation of GR nuclear transcription factor in hematopoietic cells

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Acta Pharmacologica Sinica 2007年第5期28卷 703-711页

作者： Rui-lan GAO~(2,4),Xiao-hong CHEN~2,Xiao-jie LIN~2,Xu-dai QIAN~2,Wei-hong XU~2,Beng Hock CHONG~3~2 Hematology Institution,The First Affiliated Hospital of Zhejiang Chinese Medical University,Hangzhou 310006,China ~3 Department ofMedicine,St George Hospital,University of New South Wales,Sydney,New South Wales,Australia Hematology Institution The First Affiliated Hospital of Zhejiang Chinese Medical University Hangzhou China Department of Medicine St George Hospital University of New South Wales Sydney Australia

Aim:To investigate the effects of panax notoginosides （PNS） on the proliferationof human hematopoietic stem/progenitor cells,and to explore the signaling path-way of the nuclear transcription factor of the glucocorticoid receptor （GR-NTF）initiated by PNS related with the ***:The human CD34+cellsand bone marrow nuclear cells were exposed to PNS at a concentration of 0,10,25,50,and 100 mg/L,respectively,in semi-solid culture system to observe colonyforming unite of all lineages,granulocyte,erythrocyte,and megakaryocyte （CFU-GEMM,CFU-GM,CFU-E,and CFU-MK）.Three lineages of human hematopoieticcell lines,including granulocytic HL-60,erythrocytic K562,megakaryocytic CHRF-288,and Meg-01 cells were incubated with PNS at 20 mg/L for 14 ***,dexamethasone （Dex） was used as a positive *** nuclear protein of thecells was analyzed by Western blotting with monoclonal antibodies against theamino or carboxyl terminus of *** mobility shift assay per-formed by using the32p-radiolabeled GR-NTF consensus ***:PNS promoted the proliferation of CD34+cells and significantly raised the colonynumbers of CFU-GEMM by 34.7%±16.0% over the non-PNS control （P<0.01）.PNS also enhanced the proliferation of CFU-GM,CFU-E,and CFU-MK by 39.3%±5.7%,33.3%±7.3%,and 26.2%±3.2%,***-NTF protein levels ofeither the amino or carboxyl terminus in K562,CHRF-288,and Meg-01 treated byPNS increased by 2.4-2.8 fold and 1.3-3.9 fold over the untreated ***-NTFbinding activity,initiated by either PNS or Dex,was apparently elevated to formthe complex of GR-NTF with DNA as higher density bands in K562 and CHRF-288cells,and some activity appeared as a band in HL-60 cells induced by ***:PNS displayed the action of hematopoietic growth factor-like or syn-ergistic efficacy to promote proliferation of human progenitor cells,may play arole in the upregulation of gene expression related to proliferation of hematopoi-etic cells thro

关键词： panax notoginosides hematopoietic stem/progenitor cells proliferation GR-NTF

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