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Production of proinflammatory cytokines in the human THP-1 monocyte cell line following induction by Tp0751,a recombinant protein of Treponema pallidum

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Science China(Life Sciences) 2010年第2期53卷 229-233页

作者： LIU ShuangQuan1,2,WANG ShiPing1,WU YiMou3,ZHAO FeiJun3,ZENG TieBing3,ZHANG YueJun3,ZHANG QiuGui2 & GAO DongMei1 1 Department of Parasitology,Xiangya School of Medicine,Central South University,Changsha 410078 ,China 2 The First Affiliated Hospital,University of South China,Hengyan 421001,China 3 Department of Microbiology and Immunology,University of South China,Hengyan 421001,China Department of Parasitology Xiangya School of Medicine Central South University Changsha China The First Affiliated Hospital University of South China Hengyan China Department of Microbiology and Immunology University of South China Hengyan China

The tissue destruction characteristic of syphilis infection may be caused by inflammation due to Treponema pallidum and the ensuing immune responses to the *** membrane proteins are thought to be potent inducers of inflammation during the early stages of ***,the actual membrane proteins that induce inflammatory cytokine production are not known,nor are the molecular mechanisms responsible for triggering and sustaining the inflammatory *** the present study,Tp0751 recombinant protein from *** was found to induce the production of proinflammatory cytokines,including TNF-α,IL-1βand IL-6,in a THP-1 human monocyte cell *** signal transduction pathways involved in the production of these cytokines were then further *** inhibition of TNF-a,IL-1β,or IL-6 production was observed following treatment with the SAPK/JNK specific inhibitor SP600125 or with an ERK inhibitor *** contrast,anti-TLR2 mAb,anti-CD14 mAb,and the p38 inhibitor SB203580 significantly inhibited the production of all three *** addition,pyrrolidine dithiocarbamate (PDTC),a specific inhibitor of NF-κB,profoundly inhibited the production of these ***0751 treatment strongly activated NF-κB,as revealed by Western ***,NF-κB translocation was significantly inhibited by treatment with *** results indicated that TLR2,CD14,MAPKs/p38,and NF-κB might be implicated in the inflammatory reaction caused by *** infection.

关键词： Treponema pallidum Tp0751 nuclear factor κB proinflammatory cytokines

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Relationship between the high frequency of 23S rRNA point mutations in Treponema pallidum and low serological response rate to azithromycin treatment in China

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International Journal of Dermatology and Venereology 2019年第1期2卷 6-14页

作者： Rui-Li Zhang Qian-Qiu Wang Zhi-Ju Zheng Guo-Jun Liang Li-Jia Yang Li-Gang Yang Dong-Nu Pei Shao-Chun Lin Department of Dermatology Affiliated Wuxi No.2 People's Hospital of Nanjing Medical UniversityWuxiJiangsu 214002China National Center for STD Control and Prevention China Centers for Diseases Control and PreventionHospital for Skin Diseases(Institute of Dermatology)Chinese Academy of Medical Sciences and Peking Union Medical CollegeNanjingJiangsu 210042China Department of Dermatology Dermatology Hospital of Southern Medical UniversityGuangzhouGuangdong 510091China Department of Dermatology Hainan Provincial Dermatology HospitalHaikouHainan 570100China Department of Dermatology Institute of DermatologySichuan Provincial People's HospitalChengduSichuan 611500China

Objective Although azithromycin is effective against Treponema pallidum (***),the causative agent of syphilis,recent reports indicate that the prevalence of azithromycin resistance in China is very high,which may result in the failure of *** this study,we aimed to investigate the association between azithromycin resistance and therapeutic outcomes in early syphilis *** Between February 2010 and December 2014,patients aged 18-65 years with early syphilis were *** DNA were extracted to test the presence of A2058G and A2059G ***,eligible patients were randomly assigned to receive oral azithromycin (0.5 g,once daily for 15 days) or intramuscular BPG (2.4 million units,once weekly for 3 weeks).All patients were followed up in 2 weeks and 3,6,9,and 12 months after treatment to collect demographic and clinical characteristics and laboratory *** differences on serological response,serological failure and serofast rate were compared between the two groups by Chi-square *** Among the 187 ***-infected patients enrolled,172 (92.0%) cases had a mutation associated with azithromycin resistance (A2058G,153 cases;A2059G,19 cases).During the 5-year study period,the percentage of cases enrolled with these mutations steadily increased,from 90.9% in 2010 to 95.3% in *** the 172 patients presenting with these mutations,only 78 (45.3%;all benzathine penicillin G [BPG]-treated) obtained a serological response to treatment;32.6% and 22.1% of patients presented with serological failure and serofast results,*** azithromycin-treated cases,66.3% and 33.7% had serological failure and serofast results,respectively,in contrast with 1.1% and 11.3% of BPG-treated ***,among the A2058G-and A2059G-negative patients,the serological response rates between the two treatment groups were *** multivariate analyses,patients with lower rapid plasma reagin (RPR) titers (RPR,≤ 1 ∶ 8;odds ratio [OR],

关键词： Syphilis Azithromycin Mutation Serological response Treponema pallidum

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Construction of the Eukaryotic Expression Vector for Outer Membrane Protein Tp92 from Treponema pallidum and Its Preliminary Study on the Immune Responses in New Zealand Rabbits

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Journal of Microbiology and Immunology 2004年第3期2卷 191-196页

作者：赵飞骏吴移谋刘双全余敏君 Institute of Pathogenic Biology College of Medicine Nanhua University Hengyang 421001 China

To construct the recombinant plasmid of eukaryotic expression containing Tp92 gene from Treponema pallidum and study its immunogenicity in New Zealand white rabbits. Tp92 gene was amplified from the genomic DNA of T. pallidum by polymerase chain reaction (PCR) and subcloned into appropriate site of pcDNA3.1(+) vector. After identification by sequencing and restrictive enzyme digestion, the recombinant plasmid was transfected into HeLa cells using liposome, and the expressed protein was identified by immunocytochemistry and Western blotting. After verifying that the Tp92 antigen gene fragment could be expressed in HeLa cells, 100?μg of recombinant plasmids [pcDNA3.1(+)-Tp92], 100 μg of control plasmids [pcDNA3.1(+)] or 0.5 ml PBS buffer were administered in 3 groups of New Zealand white rabbits (6 rabbits/group), and the booster immunizations were employed at 2-week interval for 3 times. ELISA assay was used for the quantitative detection of the specific antibody in the sera of rabbits, and the proliferation response of spleen cells was detected by MTT assay. It was found that the target gene Tp92 segment about 2103 bp was obtained, and the DNA sequence of Tp92 gene constructed in pcDNA3.1 (+) vector was consistent with the published nucleotide sequence. The homologies of the nucleotide and putative amino acid sequences of Tp92 gene between *** subsp. pallidum Nichols and various pathogenic treponeme strains were 95.5%-100%. The analysis of immunocytochemistry and Western blotting showed that Tp92 gene segment constructed in pcDNA3.1(+) vector could express a fusion protein with a calculated molecular mass of 77 kDa in HeLa cells and the expressed protein could react with positive blood serum from syphilis patient. The specific antibody IgG titers were observed and the highest titer was 1∶1024 in rabbits after 3 times with pcDNA3.1(+)-Tp92. The proliferation response of spleen cells were significantly higher than that of rabbits injected with pcDNA3.1(+) ( P <

关键词： Treponema pallidum DNA vaccine Tp92 gene Immun

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An Investigation on the Detection of Treponema pallidum Specific IgM Antibody

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旅行医学科学 1995年第1期1卷 45-46页

作者： Dai,Yulin Zhu,Hong Gu,Jinxiang Institute of Travel Medicine China International Travel Health care association

1 PURPOS OF THE STUDYThe article 99 in the Specific Rules for Enforcing the Frontier Health and Quarantine Law of the People’s Republic of China has indicated that the health and quarantine organization shall prevent any foreigner suffering from venereal diseases from entering the territory. Because the routine confirmatory test for syphilis fluorescent treponemal antibody ab-

关键词： Treponema Antibody TPHA routine Frontier entering territory pallidum IgG suffering

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Transcript levels of the Treponema pallidum protein Tp0751 in skin lesions of a rabbit model of early syphilis

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China Medical Abstracts(Internal Medicine) 2016年第1期33卷 19-页

作者：柯吴坚 Guangdong Prov Dermatol Hosp

Objective To trace changes in the transcript level of the Treponema pallidum(Tp)protein Tp0751 in skin lesions of a rabbit model of early *** Three New Zealand white rabbits were intracutaneously injected with 0.1 ml of Tp(Nichols Seattle strains)suspensions(10~7 treponemes/ml)at 10 sites on the shaved back to establish a model of early *** the rabbits re-

关键词： Treponema pallidum rabbit Nichols injected transcript Zealand suspensions dynamically creased

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Seroprevalence of syphilis in patients attending a tertiary care hospital in Southern India

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亚太热带生物医学杂志：英文版 2014年第12期4卷 995-997页

作者： Sadia Khan Godfred Antony Menezes Rahul Dhodapkar Belgode Narasimha Harish Department of Microbiology Amrita Institute of Medical Sciences College of Applied Medical Sciences Ha'il University Department of Microbiology Jawaharlal Institute of Postgraduate Medical Education and Research

Objective:To report our experience with two tests,anti-cardiolipin antibody test[venereal disease reasearch laboratory(VDRL) test]and specific treponemal test(Treponema pallidum hemagglutination assay),used for screening antenatal,high risk cases and cases from sexually transmitted infection in a tertiary care hospital from January 2006 till December ***:A total of 14639 samples received from various patient groups including antenatal cases,patients attending sexually transmitted disease(STD) clinic,blood donors and HIV positive patients were ***:Among the 14639 samples collected,103 were positive by VDRL *** these 89 cases were confirmed by quantitative VDRL test and Treponema pallidum hemagglutination *** cumulative seroprevalence over two years was found to be 0.61%in this *** syphilis seroprevalence reduced from 0.88%in 2006 to 0.40%in *** the various sub-populations studied,patients attending the sexually transmitted infection clinic showed a seroprevalence of 2.62%.The seroprevalence decreased significantly from 4.00%in 2006 to1.39%in ***:Our study showed a statistically significant declining rate of syphilis in STD clinics as well as the overall *** findings could be interpreted as indicators of improved programmes for prevention and management of STDs.

关键词： sexually Treponema pallidum attending transmitted screening tertiary cumulative populations prevention

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Infectious Disease

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China Medical Abstracts(Internal Medicine) 2005年第4期22卷 196-200页

2005443 Detection and genotyping of Treponemapallidum by a nested *** Heping(郑和平),et *** Skin Dis & STIs Contr Prey Center,Guangzhou *** J Dermatol 2005;38(9):***:To develop a nested PCR for the detectionof early s... 详细信息

关键词： pallidum Treponema nested Guangzhou ZHENG Guangdong Skin burden DNA hundred

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梅毒性脊髓炎1例报告

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中国脊柱脊髓杂志 2016年第4期26卷 378-379页

作者：胡越杨雪莲第二军医大学附属上海市浦东新区公利医院神经内科上海市200135

梅毒是梅毒螺旋体（treponema pallidum,TP）感染所致的一种慢性性传播疾病,神经梅毒系由TP感染人体后出现的大脑、脑膜或脊髓损害的一组临床综合征,未经治疗的早期梅毒患者约10%可发展为神经梅毒。梅毒性脊髓炎是神经梅毒的一种,是TP... 详细信息

梅毒是梅毒螺旋体（treponema pallidum,TP）感染所致的一种慢性性传播疾病,神经梅毒系由TP感染人体后出现的大脑、脑膜或脊髓损害的一组临床综合征,未经治疗的早期梅毒患者约10%可发展为神经梅毒。梅毒性脊髓炎是神经梅毒的一种,是TP累及脊膜引起的炎症反应,是梅毒性神经损害的早期表现,多发生于TP感染后2-10年,表现为急性或亚急性横贯性脊髓损害,如下肢瘫痪或无力、感觉障碍、膀胱功能异常等，在临床上少见。我院收治1例梅毒性脊髓炎患者，报道如下。

关键词：梅毒性神经梅毒脊髓损害 pallidum 早期梅毒性传播疾病下肢瘫痪梅毒感染梅毒螺旋体临床综合征

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2008～2010年献血者中梅毒血清学流行率的情况调查

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中国输血杂志 2012年第S1期25卷 100-101页

作者：毕新红 Jing Liu 杨通汉李聚林马红丽文秀琼王憬惺 Hua Shan 乌鲁木齐市血液中心 83000 美国Johns Hopkins大学云南昆明血液中心广西血液中心洛阳市中心血站绵阳市红十字中心血站中国医学科学院北京协和医学院输血研究所

目的评估5家血液中心献血者的梅毒血清学流行率增长状况。方法我们采集了参与REDS-II项目5家血液中心,从2008年1月～2010年12月的全血和血浆样品。献血后的梅毒抗体筛查采用2种不同的针对梅毒螺旋体(Trep-onema pallidum)SFDA认证的ELIS... 详细信息

目的评估5家血液中心献血者的梅毒血清学流行率增长状况。方法我们采集了参与REDS-II项目5家血液中心,从2008年1月～2010年12月的全血和血浆样品。献血后的梅毒抗体筛查采用2种不同的针对梅毒螺旋体(Trep-onema pallidum)SFDA认证的ELISA试剂完成。梅毒抗体的血清学流行率(%),以初次献血人员中两次ELISA都阳性的样品总数为分子,以各血液中心的初次献血人员献血总数为分母计算而得。梅毒抗体的血清学发生率,以两次ELISA都为阳性的重复献血者的样品总数为分子,各血液中心重复献血人员献血总数为分母计算而得。

关键词：梅毒血清学流行率梅毒抗体初次献血梅毒螺旋体 pallidum 献血人员样品总数血浆样品增长状况

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45例神经梅毒的临床分析

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中风与神经疾病杂志 2015年第2期32卷 179-180页

作者：温瑶郭阳徐锦锦吴江吉林大学白求恩第一医院神经内科吉林长春130021

神经梅毒（neurosyphilis,NS）是梅毒螺旋体（treponema pallidum,TP）侵犯脑膜和（或）脑实质引起的一种慢性中枢神经系统感染性疾病。NS临床表现复杂多样,且尚无诊断金标准,医生需要根据病史、临床表现、实验室检查及影像学检查等综... 详细信息

神经梅毒（neurosyphilis,NS）是梅毒螺旋体（treponema pallidum,TP）侵犯脑膜和（或）脑实质引起的一种慢性中枢神经系统感染性疾病。NS临床表现复杂多样,且尚无诊断金标准,医生需要根据病史、临床表现、实验室检查及影像学检查等综合判断才能明确诊断。正确、全面的认识此病,才能避免漏诊和误诊。为此,将吉林大学第一医院神经内科收治的45例神经梅毒患者临床特点总结报道如下。

关键词：神经梅毒 pallidum 影像学检查诊断金标准患者临床特点梅毒螺旋体综合判断脑脊膜水剂青霉素梅毒感染

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