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Comparative evaluation of a rapid diagnostic test,an antibody elisa,and a pldh elisa in detecting asymptomatic malaria parasitaemia in blood donors in Buea,Cameroon

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Infectious Diseases of Poverty 2017年第1期6卷 931-939页

作者： Tebit Emmanuel Kwenti Longdoh Anna Njunda Beltine Tsamul Shey Dickson Nsagha Nguedia Jules-Clement Assob Kukwah Anthony Tufon Dilonga Henry Meriki Enow George Orock Department of Medical Laboratory Sciences Faculty of Health SciencesUniversity of BueaP.B.63BueaSouthwest RegionCameroon Department of Public Health and Hygiene Faculty of Health SciencesUniversity of BueaP.B.63BueaSouthwest RegionCameroon Blood Bank Regional Hospital BueaP.B.32BueaSouthwest RegionCameroon Programme in Medicine Faculty of Health SciencesUniversity of BueaBueaSouthwest RegionCameroon

Background:In malaria endemic areas,infected blood donors serve as a source of infection to blood recipients,which may adversely affect their *** necessitates the proper screening of blood to be used for transfusion in these *** purpose of this study was to determine the prevalence of malaria parasitaemia in blood donors in Buea,Cameroon,and to evaluate the performance of a rapid diagnostic test(RDT),a malaria antibody enzyme-linked immunosorbent assay(elisa),and a Plasmodium lactate dehydrogenase(pldh)elisa in the detection of asymptomatic malaria parasitaemia in the target ***:In a prospective study conducted between September 2015 and June 2016,1240 potential blood donors were *** donors were screened for malaria parasites using Giemsa microscopy(GM)and a RDT.A sub-sample of 184 samples,comprising 88 positive and 96 negative samples,were selected for the evaluation of the pldh elisa and the antibody *** chi-square test and correlation analysis were performed as part of the statistical *** statistical significance cut-off was set at Ppldh elisa,which demonstrated the highest positive predictive value(91.6%),was generally comparable to the *** sensitivity was lowest with the antibody elisa(69.9%),which also demonstrated the highest false positive and false negative *** detection threshold for the pldh(three parasites/μl)was lower compared to the RDT(50-60 parasites/μl).Non-significant positive correlations were observed between the parasite density and the pldh titers and malaria antibody ***:Overall,the RDT and the pldh elisa demonstrated a perfectly correlated agreem

关键词： Blood transfusion Malaria RDT pldh elisa Malaria antibody elisa Comparative evaluation Sensitivity Specificity Buea Cameroon

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