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An Antimony Oxide ph Electrode for Tissue Culture Medium

稀有金属材料与工程 2006年第A3期35卷 261-263页

作者： Yang Ha Min Wang Department of Chemistry Zhejiang University Hangzhou 310028 China

A new approach named“caterpillar melt method”was developed to prepare wire type antimony oxide electrode for ph measurement in agar medium for tissue culture.A micro antimony wire was prepared from melt of the metal with the help of a glass capillary and the surface of the wire was oxidized in nitrate melt to obtain an antimony oxide electrode. Characterization results showed that the oxide layer is dense and uniform,with high physical and chemical *** electrode has a fast and stable response toward ph change for aqueous *** potential of the antimony electrode has a linear relationship with the ph of the solution (R^2=1.00) with a sensitivity of 54.1mV/*** electrode works well and is more stable in agar medium during tissue culture for ph monitoring.

关键词： ph measurement antimony oxide ph electrode tissue culture media

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Temperature coefficient of seawater ph as a function of temperature,ph,DIC and salinity

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Acta Oceanologica Sinica 2022年第6期41卷 114-118页

作者： Yubin Hu Institute of Marine Science and Technology Shandong UniversityQingdao 266237China

ph is a measure of the hydrogen ion activity in a solution,which is a function of *** normal seawater conditions,it is well ***,with an increasing interest in complex environments(e.g.,sea ice),a better understanding of the temperature change on ph under extreme conditions is *** objective of this paper was to investigate the temperature coefficient of the seawater ph(△ph/△T)over a wide range of temperature,ph,dissolved inorganic carbon(DIC)and salinity by a method of continuous ph measurement with the temperature change,and to verify the application of CO2SYS for ph conversion under extreme conditions(on the National Bureau of Standards(NBS)scale and the total proton scale).Both experimental results and CO2SYS calculations showed that△ph/△T was slightly affected by temperature over the range of 0℃ to 40℃ and by ph(at 25℃)from 7.8 to ***,when ph was out of this range,△ph/△T varied greatly with ph *** to the experimental results,changes in DIC from 1 mmol/kg to 5 mmol/kg and salinity from 20 to 105 had no significant effect on△ph/△***2SYS calculations showed a slight increase in△ph/△T with DIC on both the NBS scale and the total proton scale;and underestimated△ph/△T at high salinity(i.e.,beyond the oceanographic range)on the NBS ***,CO2SYS is still suitable for ph conversion even under extreme conditions by simply setting the input values of DIC and salinity in CO2SYS within the oceanographic range(e.g.,DIC=2 mmol/kg and S=35).

关键词： temperature coefficient ph measurement CO2SYS sea ice ocean acidification

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Observation of mitochondrial activity based on temporal and spatial ph variations measured by near-field fluorescent ratiometry

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Science China(physics,Mechanics & Astronomy) 2011年第12期54卷 2225-2229页

作者： LI YongBo SHIONHARA Ryosuke IWAMI Kentaro OHTA Yoshihiro UMEDA Norihiro Department of Mechanical Systems Engineering Tokyo University of Agriculture and Technology Tokyo 184-8588 Japan Department of Biotechnology and Life Science Tokyo University of Agriculture and Technology Tokyo 184-8588 Japan

A novel method combining dual wavelength fluorescent ratiometry with scanning near-field optical microscopy (SNOM) is proposed and developed to measure the concentration and distribution of protons in the vicinity of biological samples. This method involves immersing mitochondria in a ph-sensitive fluorescent dye solution instead of injecting the dye into the surface of the mitochondrial membrane. It uses a dual emission ph-sensitive dye and SNOM with a thermally pulled and metal-coated optical fiber probe to improve the spatial resolution. The time dependence of the fluorescence intensity ratio (FIR) under acid addition and the response of mitochondria to nutritional supplementation were studied by using this method. Activation of mitochondria and a distance-dependent delay in the FIR response were observed. The results confirmed that mitochondrial activity could be observed by using this method.

关键词： local ph ph measurement SNOM proton concentration mitochondrial membrane ATP synthesis

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