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Numerical simulation of avascular tumor growth based on p27 gene regulation

Applied Mathematics and Mechanics(English Edition) 2013年第3期34卷 327-338页

作者： Yu ZHOU Jia-wan CHEN Xiao-ning DAI Yan CAI Wei YAO Shi-xiong XU Quan LONG Department of Mechanics and Engineering Science Fudan University Shanghai 200433 P. R. China Brunel Institute for Bioengineering Brunel University Uxbridge Middlesex UB8 3PH United Kingdom

A multi-scale continuous-discrete model based on the effects of the p27 gene control is built to simulate the avascular tumor growth. At the tissue level, the continuous Eulerian model is adopted to determine the distribution of the concentration of oxygen, the extracellular matrix （ECM）, and the matrix-degradative enzyme （MDE）. At the cellular level, the discrete Lagrangien model is adopted to determine the movement, the proliferation, and the death of single tumor cells （TCs）. At the genetic level, whether a cell is committed to mitosis is determined by solving a set of equations modeling the effects of the p27 gene control. The avascular morphological evolution of the solid tumor growth is simulated, including the radius the oxygen distribution over time, and the expression. of the solid tumor, the number of the TCs, inhibiting effect＇ of the up-regulating p27 gene

关键词： tumor growth avascular p27 gene multi-scale continuous-discrete model,numerical simulation

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Effect of p27 gene Combined with pientzehuang (片仔癀) on Tumor Growth in Osteosarcoma-Bearing Nude Mice

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Chinese Journal of Integrative Medicine 2015年第11期21卷 830-836页

作者：任守松袁芳刘映红周乐天李军 Cheeloo Health Science Center of Shandong University Department of Spine Surgery Jimo City People's Hospital Department of Nephrology the Second Xiangya HospitalCentral South University

Objective： To observe the effect of p27 gene recombinant adenovirus combined with Chinese medicine pientzehuang （片仔癀） on the growth of xenografted human osteosarcoma in nude mice. Methods： Tissue transplantation was used to construct the orthotopic model of human osteosarcoma Saos-2 cell in nude mice. Thirty tumor-bearing nude mice were randomly divided into 5 groups with 6 mice in each group： blank control group （model of osteosarcoma）, empty vector group （recombinant adeno-associated virus-multiple cloning site）, pientzehuang group, p27 gene group and combined treatment group （p27 gene combined with pientzehuang）. The effect of combined treatment on human osteosarcoma was analyzed through the tumor formation, tumor volume and inhibition rate of tumor growth. The expression of p27 was measured by immunohistochemical staining and Western blot. Results： The orthotopic model of osteosarcoma in nude mice was successfully constructed. The general appearance of tumor-bearing nude mice in pientzehuang and p27 gene groups was markedly improved compared with the blank control group; and in the combined treatment group it was significantly improved compared with the pientzehuang and p27 gene groups. The tumor growth in the pientzehuang and p27 gene groups was significantly inhibited compared with the blank control group （p〈0.05）; while in the combined treatment group it was markedly inhibited compared with the pientzehuang and p27 gene groups （p〈0.05）. The rates of tumor growth inhibition were 34.1%, 56.5% and 63.8% in the pientzehuang, p27 gene and combined treatment groups, respectively. Meanwhile, the protein expression of p27 gene in the p27 gene group was significantly increased compared with the blank control group （p〈0.05）; and it was significantly increased in the combined treatment group compared with the p27 gene and pientzehuang groups （p〈0.05）. Conclusion： p27 gene introduced by adenovirus combined with pientzehuang can inhibit the growth of human osteosarcoma ce

关键词： pientzehuang p27 gene human osteosarcoma tumor growth Chinese medicine

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