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NADPH Thioredoxin Reductase C Controls the Redox Status of Chloroplast 2-Cys Peroxiredoxins in Arabidopsis thaliana

Molecular Plant 2009年第2期2卷 298-307页

作者： Kerstin Kirchsteiger Pablo Pulido Maricruz Gonzalez Francisco Javier Cejudo Instituto de Bioquimica Vegetal y Fotosintesis Universidad de Sevilla and CSIC Avda Americo Vespucio 49 41092-Sevilla Spain

Chloroplast 2-Cys peroxiredoxins （2-Cys Prxs） are efficiently reduced by NADPH Thioredoxin reductase C （NTRC）. To investigate the effect of light/darkness on NTRC function, the content of abundant plastidial enzymes, Rubisco, glutamine synthetase （GS）, and 2-Cys Prxs was analyzed during two consecutive days in Arabidopsis wild-type and ntrc mutant plants. No significant difference of the content of these proteins was observed during the day or the night in wildtype and mutant plants. NTRC deficiency caused a lower content of fully reduced 2-Cys Prxs, which was undetectable in darkness, suggesting that NTRC is the most important pathway for 2-Cys Prx reduction, probably the only one during the night. Arabidopsis contains two plastidial 2-Cys Prxs, A and B, for which T-DNA insertion lines were characterized showing the same phenotype as wild-type plants. Two-dimensional gel analysis of leaf extracts from these mutants allowed the identification of basic and acidic isoforms of 2-Cys Prx A and B. In-vitro assays and mass spectrometry analysis showed that the acidic isoform of both proteins is produced by overoxidation of the peroxidatic Cys residue to sulfinic acid. 2-Cys Prx overoxidation was lower in the NTRC mutant. These results show the important function of NTRC to maintain the redox equilibrium of chloroplast 2-Cys Prxs.

关键词： Chloroplast peroxiredoxin NADPH thioredoxin reductase C overoxidation thioredoxin Arabidopsis.

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Overoxidized poly(3,4-ethylenedioxythiophene)-gold nanoparticles-graphene-modified electrode for the simultaneous detection of dopamine and uric acid in the presence of ascorbic acid

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Journal of Pharmaceutical Analysis 2021年第6期11卷 699-708页

作者： Junqiang Pan Mei Liu Dandan Li Haonan Zheng Dongdong Zhang Department of Cardiovascular Medicine Xi'an Central HospitalXi'an710061China School of Pharmacy Xi'an Jiaotong University Health Science CenterXi'an710003China

An innovative,ternary nanocomposite composed of overoxidized poly(3,4-ethylenedioxythiophene)(OPEDOT),gold nanoparticles(Au NPs),and electrochemically reduced graphene oxide(ERGO)was prepared on a glassy carbon electrode(GCE)(OPEDOT-Au NPs-ERGO/GCE)through homogeneous chemical reactions and heterogeneous electrochemical *** morphology,composition,and structure of this nanocomposite were characterized by transmission electron microscopy,scanning electron microscopy,X-ray diffraction,and X-ray photoelectron *** electrochemical properties of the OPEDOT-Au NPs-ERGO/GCE were investigated by cyclic voltammetry using potassium ferricyanide and hexaammineruthenium(III)chloride redox probe *** modified electrode shows excellent electro-catalytic activity for dopamine(DA)and uric acid(UA)under physiological p H conditions,but inhibits the oxidation of ascorbic acid(AA).Linear voltammetric responses were obtained when DA concentrations of approximately 4.0-100μM and UA concentrations of approximately 20-100μM were *** detection limits(S/N=3)for DA and UA were 1.0 and 5.0μM,respectively,under physiological conditions and in the presence of 1.0 m M of *** developed method was applied to the simultaneous detection of DA and UA in human urine,where satisfactory recoveries from 96.7%to 105.0%were *** work demonstrates that the developed OPEDOT-Au NPs-ERGO ternary nanocomposite,with its excellent ion-selectivity and electro-catalytic activity,is a promising candidate for the simultaneous detection of DA and UA in the presence of AA in physiological and pathological studies.

关键词： Graphene Poly(3,4-ethylenedioxythiophene) overoxidation Dopamine Uric acid Ascorbic acid

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