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中国动物学会寄生虫学专业委员会第十届全国寄生虫学青年工作者学术研讨会

作者： Dandan Hu Guangping Huang Chunhui Duan Jingxia Suo Xianyong Liu Xun Suo National Animal Protozoa Laboratory College of Veterinary Medicine China Agricultural University

Objective: Chicken coccidiosis, caused by Eimeria sp., leads to huge economic losses in the poultry industry. This disease could be controlled by vaccination with different Eimeria precocious lines, which was selected by multiple continued propagation in chicken. Recent researches on Plasmodium revealed that a transcription factor apicomplexanAP2(ApiAP2) played a crucial role in the sexual commitment in its lifecycle. Here, to characterize the orthologous ApiAP2 gene in E. tenella(EtAp2) and its potential role in regulating Eimeria precocious, we constructed a transgenic E. tenella line over-expressing EtAp2. Methods: The transfection plasmid consists of a pyrimethamine selection maker(Toxoplasma gondii dihydrofolate reductase-thymidylate synthase) and fused immediately with an enhanced YFP reporter gene, the complete EtAp2 coding sequence was then linked by a "self-cleaving" 2A peptide sequence. This plasmid was regulated by the E. tenella Histone 4 promoter and C-terminal untranslated region of E. tenella Actin. Purified E. tenella sporozoites were transfected with the over-expressing vector and then inoculated into the MDBK bovine kidney cells and chickens. Results: The total transfection rate was 0.07% in the first generation oocysts measured by flow cytometry analysis. Both the transiently transfected sporozoites and the stable transfected E. tenella-Ap2 oocysts were expressing e YFP in a nuclear expressing manner. Conclusion: We constructed a transgenic E. tenella line over-expressing EtAp2 successfully. Our results established a basis for the further study on mining the downstream genes regulated by this transcription factor, which might helpful in understanding the mechanism of precocious.

关键词： Eimeria tenella apicomplexan AP2 over-expressing

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GbLMI1 over-expression improves cotton aboveground vegetative growth

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Journal of Integrative Agriculture 2024年第10期23卷 3457-3467页

作者： Zhili Chong Yunxiao Wei Kaili Li Muhammad Aneeq Ur Rahman Chengzhen Liang Zhigang Meng Yuan Wang Sandui Guo Liangrong He Rui Zhang College of Agronomy Tarim UniversityAlar 843300China Biotechnology Research Institute Chinese Academy of Agricultural SciencesBeijing 100081China

Leaves are the main organ for photosynthesis and organic synthesis in *** shape has important effects on photosynthetic efficiency and canopy formation,thereby affecting cotton *** studies have shown that LMI1(LATE MERISTEM IDENTITY1)is the main gene regulating leaf *** this study,the LMI1 gene was inserted into the 35S promoter expression vector,and cotton plants overexpressing LMI1(OE)were obtained through genetic *** analysis of the biological traits of the T_1 and T_2 populations showed that compared to the wild type(WT),OE plants had significantly larger leaves,thicker stems and significantly greater dry ***,plant sections of the main vein and petiole showed that the numbers of cells in those tissues of OE plants were significantly *** addition,RNA-seq analysis revealed the differential expression of genes related to gibberellin synthesis and NAC gene family(genes containing the NAC domain)between the OE and WT plants,suggesting that LMI1 is involved in secondary wall formation and cell proliferation,which promotes stem ***,Gene Ontology(GO)analysis revealed enrichment in the terms of calcium ion binding,and Kyoto Encyclopedia of Genes and Genomes(KEGG)analysis showed enrichment in the terms of fatty acid degradation,phosphatidylinositol signal transduction system,and c AMP(cyclic adenosine monophosphate)signal *** results suggested that LMI1 OE plants are responsive to gibberellin hormone signals,and have altered messenger signals(c AMP,Ca^(2+))which amplify this function,to promote stronger aboveground vegetative *** study found the LMI1 greatly increased the vegetative growth in cotton,which is the basic requirement for higher yield.

关键词： LMI1 over-expressing cotton vegetative growth

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