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Estimating out-crossing Rate of Bg 379-2 Using Morphological Markers and Confirmation by Molecular Markers

Rice science 2012年第2期19卷 166-168页

作者： L.H.M.Y.K.SOMARATNE A.S.M.T.ABAYAWICKRAMA I.P.WICKRAMASINGHE W.L.G.SAMARASINGHE Department of Agricultural Biology Faculty of AgricultureUniversity of PeradeniyaSri Lanka Rice Research and Development Institute BatalagodaIbbagamuwaSri Lanka

Rice is largely self-fertilized and accordingly a field population of rice is completely composed of near homozygotes. Due to the emergence of off-types, homozygosity will be affected. With the time, this will cause the reduction of genetic purity in some rice varieties. One of the reasons has been suspected to be the high out-crossing frequencies of such varieties. Studies were conducted at the Rice Research and Development Institute, Batalagoda, Sri Lanka to estimate the out-crossing rate of Bg 379-2, a variety having the problem of maintaining genetic purity. Bg 379-2 was allowed to out-cross with Bg 450 and the number of out- crossed plants were counted using dominant morphological markers such as short-round grain and purple culm of pollen donor. A molecular confirmation of out-crossing was also performed using sequence tagged site （STS） molecular marker pTA248. The variety Bg 379-2 showed a potential out-crossing rate of 3.41% and an average out-crossing rate of 1.29% using dominant morphological markers. Polymorphism was cleady detected between parents and out-crossed plants as well as selfed plants of Bg 379-2 using their banding patterns. A similar study can be performed to determine the out-crossing rates of other varieties which show high percentage of off-types in the population for the better understanding of the breeding behavior of the varieties.

关键词： rice genetic purity out-crossing marker polymorphism

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