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Study on New pretreatment.Combining Dilute Acid Treatment and Organic Solvent Water / Ethanol for Biorefinery： Application to Switch Grass

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Journal of Life Sciences 2010年第3期4卷 25-32页

作者： Dachun Gong Yingping Huang M. Holtman Kevin Ruiping Li Franqui-Espiet Diana J. Orts William Alan G.Macdiarmid Institute of Renewable Energy China Three Gorges University Yichang 443002 China Western Regional Research Center Agricultural Research Service US Department of Agriculture Albany CA 94 710 USA

Switch grass was developed as a pioneer energy crop in USA with great industrial prospect. It contains about 60% sugars and 18% lignin. The purpose of this research is to find pretreatment.process to fractionate cellulose, hemicellulose and lignin from switch grass to obtain much more useful chemicals and enhance the residue solid to be hydrolyzed by enzymes. The six different pretreatment.methods were studied, such as hot water pretreatment.（HWP） alone, dilute acid pretreatment.（DAP）, ammonia pretreatment.（AP）, lime pretreatment.（LP）, organosolv water / ehanol pretreatment.（OWEP）, and organosolv water / acetone pretreatment.（OWAP）. It was the best method combining DAP with OWEP because the hemicellulose sugars were recovered in the first residual liquid while a varied amount of cellulose was retained in the residual solid and the lignin fraction was obtained by simply adjusting the pH from the second liquid. The result shows the optimal two-stage process consisted of the first stage DAP at 428 K for 7 min with 0.8% sulfuric acid, resulting in 79.82% glucose recovery yield and 98.74% xylose removal and the second stage OWEP at 468K for 20 min in 45% （v / v） ethanol with 0.4% NaOH, resulting in 62% total glucose yield 99% xylose and 80% lignin removal. After enzymatic hydrolysis, the glucose yield was up to 92.6%, compared with 16% yield from untreated switch grass. Scanning electron microscopy （SEM） highlighted the differences in switch grass structure from the various pretreatment.methods during biomass fractionation.

关键词： Switch grass fractionation dilute acid pretreatment organosolv pretreatment.

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