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An integrative study of larval organogenesis of American shad Alosa sapidissima in histological aspects

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Chinese Journal of Oceanology and Limnology 2016年第1期34卷 136-152页

作者：高小强洪磊刘志峰郭正龙王耀辉雷霁霖 College of Fisheries Ocean University of ChinaQingdao 266003China Key Laboratory for Sustainable Development of Marine Fisheries Ministry of AgricultureQingdao Key Laboratory for Marine Fish Breeding and BiotechnologyYellow Sea Fisheries Research InstituteChinese Academy of Fishery SciencesQingdao 266071China Jiangsu Zhongyang Group Nantong 226600China

We describe organogenesis at a histological level in American shad( Alosa sapidissima) larvae from 0 until 45 days after hatching(DAH). Larval development was divided into four stages based on the feeding mode,external morphological features,and structural changes in the organs: stage 1(0–2 DAH),stage 2(3–5 DAH),stage 3(6–26 DAH) and stage 4(27–45 DAH). At early stage 2(3 DAH),American shad larvae developed the initial digestive and absorptive tissues,including the mouth and anal opening,buccopharyngeal cavity,oesophagus,incipient stomach,anterior and posterior intestine,differentiated hepatocytes,and exocrine pancreas. The digestive and absorptive capacity developed further in stages 2 to 3,at which time the pharyngeal teeth,taste buds,gut mucosa folds,differentiated stomach,and gastric glands could be observed. Four defined compartments were discernible in the heart at 4 DAH. From 3 to 13 DAH,the excretory systems started to develop,accompanied by urinary bladder opening,the appearance and development of primordial pronephros,and the proliferation and convolution of renal tubules. Primordial gills were detected at 2 DAH,the pseudobranch was visible at 6 DAH,and the filaments and lamellae proliferated rapidly during stage 3. The primordial swim bladder was first observed at 2 DAH and started to inflate at 9 DAH; from then on,it expanded constantly. The spleen was first observed at 8 DAH and the thymus was evident at 12 DAH. From stage 4 onwards,most organs essentially manifested an increase in size,number,and complexity of tissue structure.

关键词： Alosa sapidissima larval development ontogeny histology organ differentiation

Plant Developmental Biology in China: Past, Present and Future

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Acta Botanica Sinica 2002年第9期44卷 1085-1095页

作者：许智宏种康北京大学生命科学学院北京100871 中国科学院植物研究所光合作用与环境分子生理学重点实验室北京100093

Plant development is a process from germination of seed to vegetative growth, flower initiation and development, fertilization and finally to the next generation seed formation. A lot of achievements have been obtained in plant developmental biology in China. Since the 1990's, those young generation scientists returned to China from abroad to use molecular and genetic techniques to study morphological, physiological and biochemical process of plant development. The present paper reviews the progress in some research area of plant developmental biology in the past decades and also prospects the chance and future of plant developmental studies, due to the recent advances of plant genome sequencing and functional genomics in China under the international research background.

关键词： developmental biology organ differentiation flowering physiology sexual differentiation fertilization biology seed physiology

Primary study on anther culture of balsum pear for callus and organ formation

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Journal of Life Sciences 2008年第1期2卷 12-18页

作者： LI Huan-xiu CHEN Jia ZHENG Yang-xia YAN Ze-sheng HE Yan Department of Horticulture College of Forestry and Horticulture Sichuan Agriculture University Ya＇an Sichuan 625014 China

The balsum pear （Momordica charantia L. ） anthers in the monokaryotic stage of microspore development were cultured in this experiment. Different Plant growth regulators＇ combinations, base media and carbon sources were studied for callus formation and organ differentiation from balsum pear anthers. The result showed that the best media for callus inducement was： MS＋BA 0.5 mg/L＋NAA 0.2 mg/L＋ 2, 4-D 0.5 mg/L＋KT 2.0 mg/L, with 3% sugar and 0.8% agar. The best media to induce roots from balsum pear anther callus was： MS＋NAA 0.05 rag/L＋ KT 0.5 rag/L, with 3% sugar and 0.8% agar. Most of adventitious roots from callus were triploid（2N=3X=33）

关键词： balsum pear (Momordica charantia L. ) antherculture callus inducement organ differentiation

Effect of Different Plant Growth Regulators on Callus Induction and in vitro Rapid Propagation of Wild Petunia Juss.

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Agricultural Science & Technology 2012年第5期13卷 931-934页

作者： Yan ZHAO Na XU Zhongyou MA Wei LIU School of Life Science Anhui Science and Technology UniversityFengyang 233100China

In this study,the seeds of wild Petunia *** used as explants to investigate the optimal condition for tissue *** different kinds and concentrations of growth regulators were adopted to produce more multiple bud clumps,callus or roots in this *** experiments may provide experimental foundation for the rapid propagation technology and establishment of tissue culture system for wild Petunia Juss.

关键词： Petunia Juss. organ differentiation Rapid propagation Plant growth regulators