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The use of ultrasound probes to monitor multi-phase behavior in opaque systems

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Particuology 2019年第4期17卷 91-97页

作者： Weibin Kong Shuo Li Hui Ke Huili Zhang Jan Baeyens College of Life Science and Technology Beijing University of Chemical Technology Beijing 100029 China College of Chemical Engineering Beijing University of Chemical Technology Beijing 100029 China European Powder and Process Technology 3120 Tremelo Belgium Beijing Advanced Innovation Centre for Smart Matter Science and Engineering Beijing University of Chemical Technology Beijing 100029 China

The detection of phenomena occurring inside multiphase opaque reactors is difficult. The present work investigated the novel application of a non-intrusive ultrasound monitoring technique to the complex case of gas-solid fluidized beds. Comparisons of data obtained from video-imaging and theoretical calculations with the ultrasound signal data demonstrate that the ultrasound system is a reliable means of characterizing essential bubble properties by measuring the point bubble frequency, when applying a special protocol during signal treatment. Additional uses in different multiphase systems are further described.

关键词： Ultrasound monitoring Non-intrusive Multiphase system opaque

Petrogenesis of opaque assemblages in the Ningqiang carbonaceous chondrite

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Science China Earth Sciences 2007年第6期50卷 886-896页

作者： HSU WeiBiao Laboratory for Astrochemistry and Planetary Sciences Purple Mountain ObservatoryNanjing 210008China

Numerous round to oblate opaque assemblages (OAs) are found in chondrules and matrix of the Ningqiang carbonaceous chondrite. They are mainly composed of Ni-rich metal,magnetite,Fe,Ni-sulfides,with minor amounts of phosphate,phosphoran-olivine,pyroxene and trace amounts of nano-sized platinum-group metal alloys. The mineralogy of Ningqiang OAs is very similar to that of OAs previously reported in Ca,Al-rich inclusions of CV chondrites. Being a rare mineral phase in nature,phosphoran-olivine is thought to form by nonequilibrium reactions between P-bearing molten metal and olivine crystals during rapid cooling. Its occurrence in Ningqiang OAs indicates that the precursor of OAs was locally produced during chondrule formation,rather than directly condensed from the solar nebula as previously thought. The petrographic and mineralogical characteristics of Ningqiang OAs reveal that OAs formed by low temperature alterations of pre-existing homogeneous alloys within chondrules on a planetary body.

关键词： opaque assemblages phosphoran-olivine Ningqiang carbonaceous chondrite petrogenesis

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Beneficial effects of vincamine with thioctic acid and lutein on retinal and optic nerve functions in an opaque media

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International Journal of Ophthalmology(English edition) 2019年第9期12卷 1450-1455页

作者： Mona Abdelkader Sameh Saleh Tharwat Mokbel Hossam Abouelkheir Ayman Abd El Ghafar Mansoura Ophthalmic Center Mansoura Medicine Hospital Faculty of Medicine Mansoura University

AIM: To detect whether the combination of vincamine, thioctic acid and lutein will improve the retina and optic nerve functions in cases of an opaque media with an optic nerve and/or a retinal defect or ***: Totally 2000 patients(2000 eyes) of corneal opacities with defects in the optic nerve or/and the retinal functions were studied. Every patient received three types of drugs: thioctic acid with cyanocobalamine, vincamine, and lutein. The drugs were given daily for 3-12 mo according to patient’s responses. Full field flash electroretinogram(ERG) and flash visual evoked potential(VEP) were done before treatment and at 1, 3, 6, and 12 mo sequentially. Patients were followed up for 12 ***: In the 2000 eyes, 1000 eyes had both moderate optic nerve and retinal function defects;and 840 eyes out of the 1000 improved with the medical treatment. Another 500 eyes out of the 2000 eyes had extinguished retinal function with normal optic nerve function and only 125 eyes of them improved. The 290 out of the 2000 eyes had severe defects in optic nerve with normal retinal function and 130 of them improved. Another 210 eyes have mild optic nerve and retinal function defects and 194 ***: The combination of vincamine, thioctic acid with cyanocobalamine, and lutein improved both retina and optic nerve functions mainly in mild and moderate defect than in severe cases.

关键词： retina optic nerve opaque media thioctic acid with cyanocobalamine vincamine lutein

Paired eye-control study of unilateral opaque bubble layer in femtosecond laser assisted laser in situ keratomileusis

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International Journal of Ophthalmology(English edition) 2019年第4期12卷 654-659页

作者： Cheng-Hua Wei Qiao-Yun Dai Li-Xin Mei Yao Ge Peng-Fei Zhang E Song Department of Ophthalmology the Second Affiliated Hospital of Soochow University Department of Ophthalmology the First Affiliated Hospital of Wannan Medical College Department of Ophthalmology Lixiang Eye Hospital of Soochow University

AIM: To investigate the predictive factors of the opaque bubble layer(OBL) compared to the fellow eye of same patients in FS200 femtosecond laser assisted laser in situ keratomileusis(FS-LASIK).METHODS: This study consisted of 60 consecutive patients(120 eyes) with unilateral OBL during FS-LASIK. Eyes were divided into OBL(the OBL eyes) and OBL-free groups(the fellow eyes) based on the occurrence of OBL. The preoperative demographic data, refraction, keratometry, corneal astigmatism, pachymetry, intraocular pressure and intraoperative data including the outlet location of gas diffusing canal were collected. Conditional logistic regression analysis was performed to find the associated factors with OBL in the two groups by determining odds ratios(OR) and 95%***: The preoperative demographic data, mean spherical errors, mean K value, suction time, intraocular pressure and central cornea thickness were not significantly different between the two groups. The outlet location of gas diffusing canal(P<0.01, OR 7.16, 95%CI 2.53-20.32) and the corneal astigmatism(P=0.013, OR 0.13, 95%CI 0.03-0.65) were significantly associated with the incidence of OBL by multivariate logistic regression analysis. Visual acuity, efficacy, and safety were comparable between the two groups two months after surgery except for a slightly lower predictability value for the hard OBL ***: The reduction of the incidence of OBL is obvious when the outlet of gas diffusing canal located at the posterior border of the corneoscleral limbus. This is probably consequent to more effectiveness of gas diffusing canal. Corneal astigmatism is also an independent protective factor for OBL formation.

关键词： opaque bubble layer femtosecond laser LASIK location of gas diffusing canal

A white opaque substance-positive gastric hyperplastic polyp with dysplasia

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World Journal of Gastroenterology 2013年第26期19卷 4262-4266页

作者： Hiroya Ueyama Kenshi Matsumoto Akihito Nagahara Ryosuke Gushima Takuo Hayashi Takashi Yao Sumio Watanabe Department of Gastroenterology Juntendo University School of Medicine Department of Human Pathology Juntendo University School of Medicine

The endoscopic findings of gastric hyperplastic polyps (HPs) with dysplasia have not been well-defined, and the clinical significance of these lesions, including their malignant potential, is unclear. In this report, we describe a case of a white opaque substance (WOS)positive gastric HP with dysplasia. A 76-year-old woman was referred to our hospital for endoscopic resection of a gastric HP. Upper endoscopy revealed a 25-mm whitish and reddish polypoid lesion on the greater curvature in the lower third of the stomach. The whitish part was diagnosed as a WOS using conventional and magnifying endoscopy with narrow band imaging. An examination of the biopsy specimen indicated that the lesion was a typical gastric HP. However, because of its color and the presence of a WOS, we suspected that this lesion was an atypical gastric HP. Therefore, we performed a polypectomy. Histopathologically, diffuse lowto high-grade dysplasia was found on the surface of the polyp. We performed immunohistochemical staining using a monoclonal antibody specific for adipophilin as a marker of lipid droplets (LDs). LDs were detected in approximately all of the neoplastic cells, especially in the surface epithelium of the intervening apical parts and were located in the subnuclear cytoplasm of the neoplastic cells. According to endoscopic and histopathological findings, the WOS-positive epithelium indicated dysplasia of the gastrointestinal phenotype, which could absorb lipids. The presence of a WOS in a gastric HP may be considered an endoscopic finding that is predictive of the neoplastic transformation of a gastric HP. We suggest that a WOS-positive gastric HP should be resected endoscopically to investigate its neoplastic transformation.

关键词： Gastric hyperplastic polyp with dysplasia White opaque substance Adipophilin Lipid droplet Narrow band imaging

opaque 2基因微卫星标记与玉米籽粒赖氨酸含量的关系

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作物学报 2004年第8期30卷 757-761页

作者：姜伟李新海李明顺田清震张世煌中国农业科学院作物育种栽培研究所

利用opaque 2 (o2 )基因序列内的微卫星标记phi0 5 7、phi112和umc10 6 6检测优质蛋白和普通玉米自交系CA335及黄早四 ,发现phi0 5 7是共显性标记 ,且具有明显的多态性。利用标记phi0 5 7对BC1F1回交群体 [(CA335×黄早四 )×CA335 ]的 ... 详细信息

利用opaque 2 (o2 )基因序列内的微卫星标记phi0 5 7、phi112和umc10 6 6检测优质蛋白和普通玉米自交系CA335及黄早四 ,发现phi0 5 7是共显性标记 ,且具有明显的多态性。利用标记phi0 5 7对BC1F1回交群体 [(CA335×黄早四 )×CA335 ]的 2 2 4个单株进行检测 ,两种基因型o2o2和o2O2的分离比为 1∶1 2 4 ,符合孟德尔遗传分离比例。进一步将群体内同一基因型o2O2或o2o2植株上的籽粒混合测定赖氨酸含量 ,发现o2o2基因型玉米籽粒的赖氨酸含量 (0 4 2 8% )显著大于o2O2基因型 (0 334% )。研究表明利用微卫星标记phi0 5 7辅助选择玉米o2o2基因型 ,可以提高优质蛋白玉米的选择效率。

关键词： opaque 2基因微卫星标记玉米赖氨酸含量分子标记辅助选择

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opaque2转录因子对玉米α-醇溶蛋白基因家族成员表达的影响

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江苏农业学报 2015年第6期31卷 1224-1231页

作者：李国锋葛敏吕远大江苏省农业科学院六合基地江苏南京210014 江苏省农业科学院农业生物技术研究所/江苏省农业生物学重点实验室江苏南京210014

醇溶蛋白是玉米主要的储存蛋白,其中α-醇溶蛋白含量最为丰富,对玉米籽粒品质有重要影响。本研究利用全基因组数据鉴定α-醇溶蛋白基因家族,并从基因保守motif、玉米籽粒不同发育时期基因的表达谱以及转录因子opaque2对该家族成员表达... 详细信息

醇溶蛋白是玉米主要的储存蛋白,其中α-醇溶蛋白含量最为丰富,对玉米籽粒品质有重要影响。本研究利用全基因组数据鉴定α-醇溶蛋白基因家族,并从基因保守motif、玉米籽粒不同发育时期基因的表达谱以及转录因子opaque2对该家族成员表达的影响3个方面分析玉米α-醇溶蛋白基因家族。结果显示,玉米参考基因组中存在39个α-醇溶蛋白基因,其中z1A、z1B、z1C和z1D 4个亚族成员分别为12个、8个、14个和5个。基因保守motif分析发现该家族基因序列高度保守。表达谱分析结果显示该家族表达模式差异较大,其中z1A亚族在籽粒发育时期表达量最高。利用RNA-seq数据剖析在种子发育阶段opaque 2(O2)野生型和突变体编码该家族成员的表达差异,结果显示O2突变体中z1C亚族成员表达显著下调,z1A亚族表达略有下调,z1B和z1D亚族成员表达量没有显著的变化。本研究在重新注释α-醇溶蛋白家族成员信息的基础上,对该家族的表达谱进行了分析,为今后玉米籽粒品质育种提供了必要遗传信息。

关键词：玉米 α-醇溶蛋白基因家族表达谱 opaque 2

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玉米EMS突变体库创建及opaque和CENH3基因的TILLING分析

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玉米EMS突变体库创建及opaque和CENH3基因的TILLING分析

作者：杨媛媛四川农业大学

学位级别：硕士

玉米种质是玉米改良的基础。随着生产水平的提高和新品种的推广,玉米育种所利用的基因集中到少数种质上,由此导致遗传多样性严重耗失。优良种质的集中利用及种质基础的日趋狭窄一直是制约我国玉米育种取得突破性进展的瓶颈。利用各种物... 详细信息

玉米种质是玉米改良的基础。随着生产水平的提高和新品种的推广,玉米育种所利用的基因集中到少数种质上,由此导致遗传多样性严重耗失。优良种质的集中利用及种质基础的日趋狭窄一直是制约我国玉米育种取得突破性进展的瓶颈。利用各种物理化学因素诱导突变,可创造全新的遗传类型,丰富玉米种质资源。但是,传统的诱变育种存在诱变频率较低、无益突变较多、有益突变的选择困难等多方面的问题,多年来进展不大。基因组靶向定位诱导损伤技术(targeting induced local lesions in genomes, TILLING)的发展为玉米定向突变和反向遗传学研究提供了一种全新的方法。TILLING技术将诱发产生高频率点突变的化学诱变方法与定向PCR筛选技术以及双色红外荧光检测技术有效结合,利用化学诱变剂甲基磺酸乙酯(Ethylmethane sulfonate, EMS)诱发产生一系列点突变,再用设计的特异性引物进行RT-PCR扩增及高分辨率熔解曲线(High resolution melting, HRM)分析,快速有效地从突变群体中鉴定出点突变,其突变性状涉及植株结构、器官形态、生长发育、产量品质因素以及病虫害抗性等方面,为玉米品种改良提供新颖种质。本研究利用EMS花粉诱变技术构建突变体库,通过对诱变后代田间性状的观察,筛选有益突变体;同时,在M1代株系中,对影响玉米胚乳蛋白品质的转录因子基因opaque和影响有丝分裂过程的着丝粒特异组蛋白3基因CENH3进行TILLING分析,以期为优质蛋白玉米育种和新型单倍体诱导系创制提供全新的种质。本研究选用B73和Mo17两个模式自交系,以及18-599(红)和18-599(白)两个西南生态区骨干自交系为供试材料,分别用EMS体积分数为0(对照)、0.50×10-3、0.67x10-3、1.00×10-3、1.67×10-3、2.00×10-3的EMS-石蜡油处理液处理花粉45min,做诱变预实验,收获后统计结实率,以此确定适当的EMS诱变浓度。根据预实验结果,选择EMS体积分数为1.00x10-3的EMS-石蜡油处理液处理自交系18-599(红)的花粉。田间性状观察结果表明,诱变M1代出苗率和成株率明显低于对照;苗期外观上发生了较大变异,主要有叶色变异、叶形变异等;M2代结实情况也比对照差,且籽粒表型亦出现较大变异,出现了类似甜玉米的皱缩籽粒等有益突变。提取诱变M1代的基因组DNA,构建8倍DNA池。根据opaque和CENH3基因的外显子序列设计13对特异引物,对DNA池做Real-time PCR扩增及HRM分析,筛选出8个突变样品。经过测序验证,以上8个突变样品与对照相比,有3个发生了碱基突变,其中两个在内含子区域,对基因的表达应该不会造成影响;opaque基因第4外显子区域的第3134bp核苷酸,由G：C变化为A：T,导致编码的氨基酸由精氨酸变化为组氨酸。生物信息学分析表明,该突变的氨基酸位于02蛋白的bZIP结构域,参与构成了02碱性域223和245残基之间的NLSB二分结构,而该结构是02核寻靶的主要因子。因此,该氨基酸的变化可能会影响02蛋白运输到核中的效率,不仅可能影响转录因子进入细胞核的过程,也可能改变转录因子的活性和与DNA结合的能力,进而影响转录因子opaque在胚乳中的特异表达,有待进一步鉴定其对胚乳蛋白品质的影响。

关键词：玉米 EMS诱变 TILLING opaque CENH3

两种opaque技术和两种合金表面状况对金属陶瓷结合力的影响

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国际生物医学工程杂志 1996年第1期 54-54页

作者：刘玲张丽霞

001两种opaque技术和两种合金表面状况对金属陶瓷结合力的影响［英〕／RaKePC…JProsthetDent．－1995，74（1）．－8瓷熔附金属（porcelain－fused－to－metal）牙冠桥在牙体和牙列缺损修复中最常采用。制和临床应用这类修复体时常出... 详细信息

001两种opaque技术和两种合金表面状况对金属陶瓷结合力的影响［英〕／RaKePC…JProsthetDent．－1995，74（1）．－8瓷熔附金属（porcelain－fused－to－metal）牙冠桥在牙体和牙列缺损修复中最常采用。制和临床应用这类修复体时常出现瓷从金属基体（substrate）上剥落的...

关键词：表面状况金属陶瓷陶瓷合金 opaque 结合力两种