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Effect of Electroacupuncture on Spermatogenesis in Rats with oligozoospermia of Insufficiency of Shen(Kidney) Essence Syndrome

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Chinese Journal of Integrative Medicine 2019年第4期25卷 292-297页

作者： CUI Tian-wei QIN Mao LIU Bao-xing GAO Yun-xiao MA Wen-jing ZHANG Xiu-ping Department of Andrology China-Japan Friendship HospitalBeijing(100029)China Department of Andrology Chongqing Health Center for Women and ChildrenChongqing(400013)China

Objective: To assess the effect of electroacupuncture(EA) on expression of cytoskeletal proteins from Sertoli cells(SCs) and spermatogenesis in rats with oligozoospermia of insufficiency of Shen(Kidney)essence syndrome(OIKES).Methods: Twenty healthy male Sprague-Dawley rats were randomly assigned to four groups using a random number table: control,tripterygium glycosides(TG) treatment,sham and EA groups(n=5 in each group).A rat model of OIKES was established by oral gavage with *** EA group was treated with TG and received EA at Shenshu(BL 23) and Zusanli(ST 36) acupoints for 20 min,once daily for 30 days,while the sham group received EA at identical acupoints with skin penetration without *** 30 days,the ?nal body weight and coef?cients for the testis and epididymis were calculated and sperm parameters were *** analyses were performed to detect expression of vimentin and α-tubulin in SCs and proliferating cell nuclear antigen(PCNA) immunoreactivity in germ *** in germ cells was quanti?ed by the transferase biotin-dUTP nick end labeling ***: Compared with the control group,the final body weight and testis/epididymis coefficients of rats in the TG-treated group were not significantly different,but the sperm count and motility were lower(P<0.05).Expressions of vimentin and α-tubulin were also signi?cantly weaker(P<0.01).The PCNA immunoreactivity of germ cells was decreased(P=0.059),whereas the apoptotic index of germ cells was increased signi?cantly(P<0.01).In contrast,EA at BL 23 and ST 36 acupoints signi?cantly improved the ?nal body weight as well as the sperm count,concentration and motility(P<0.01 or P<0.05).EA increased expression of vimentin and α-tubulin in SCs markedly,and signi?cantly enhanced PCNA immunoreactivity with decreased apoptosis in germ cells(P<0.01 or P<0.05).Conclusions: EA at BL 23 and ST 36 acupoints has protective effects on spermatogenesis in rats with *** effect seems to

关键词： electroacupuncture Sertoli cells cytoskeletal proteins oligozoospermia insufficiency of Shen(Kidney)essence syndrome

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Varicocele repair in severe oligozoospermia: A case report of post-operative azoospermia

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World Journal of Clinical Cases 2014年第4期2卷 94-96页

作者： Marco Milone Mario Musella Miguel Emilio Sosa Fernandez Paola Maietta Annarita Sasso Loredana Maria Sosa Fernandez Laura Virginia Sosa Fernandez Francesco Milone Department of Advanced Biomedical Science University of Naples "Federico Ⅱ"80131 Naples Italy Embryos IVF Center in Battipaglia84100 Salerno Italy

Varicocele has been implicated as a cause in 35%-50% of patients with primary infertility and up to 81% of men with secondary infertility. Although a large number of reports have shown improvement in the semen parameters after correction of varicocele, other studies have suggested no benefit. We report the first case of azoospermia after surgery in a young infertile male patient with left-sided varicocele and severe oligozoospermia undergoing laparoscopic varicocelectomy. A pregnancy was only achieved with assisted reproductive technology because semen cryopreservation was performed before surgery. In the light of the above, the deterioration of sperm count after varicocele repair in patients with severe oligozoospermia could be due to irreversible impairment of spermatogenesis of such patients, together with the possible temporary damage of the surgical repair. This possible complication could therefore turn the severe oligozoospermia into an indication to perform cryopreservation before surgery, on both clinical and medico-legal grounds. Further research is needed before drawing definitive conclusions regarding the management of varicocele-related severe oligozoospermia.

关键词： Varicocele Fertility Semen Cryopreservation oligozoospermia

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Genetic polymorphisms of CYP2D6*10 and the effectiveness of combined tamoxifen citrate and testosterone undecanoate treatment in infertile men with idiopathic oligozoospermia

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2015年第3期16卷 191-197页

作者： Kai-fa TANG Yi-li ZHAO Shang-shu DING Qi-fei WU Xing-yang WANG Jia-qi SHI Fa SUN Jun-ping XING Department of Urology Affiliated Hospital of Guiyang Medical College Department of Urology the First Affiliated Hospital of Medical College of Xi'an Jiaotong University

Tamoxifen citrate, as the first line of treatment for infertile men with idiopathic oligozoospermia, was proposed by the World Health Organization （WHO）, and testosterone undecanoate has shown benefits in semen values. Our objective was to assess the effectiveness of treatment with tamoxifen citrate and testosterone un- decanoate in infertile men with idiopathic oligozoospermia, and whether the results would be affected by polymor- phisms of CYP2D6＊10. A total of 230 infertile men and 147 controls were included in the study. Patients were treated with tamoxifen citrate and testosterone undecanoate. Sex hormone, sperm parameters, and incidence of spontaneous pregnancy were detected. There were no significant differences between the control and patient groups with respect to CYP2D6＊10 genotype frequencies （P〉0.05）. The follicle-stimulation hormone （FSH）, luteinizing hormone （LH）, and testosterone （T） levels were raised, and sperm concentration and motility were increased at 3 months and became significant at 6 months, and they were higher in the wild-type allele （C/C） than in the heterozygous variant allele （C/T） or homozygous variant allele （T/T） subgroups （P〈0.05）. In addition, the percentage of normal morphology was raised at 6 months, and represented the highest percentage in the C/C subgroup （P〈0.05）. The incidence of spontaneous pregnancy in the C/C subgroup was higher than that in the C/T or T/T subgroups （P〈0.01）. This study showed that the CYP2D6＊10variant genotype demonstrated worse clinical effects in infertile men with idiopathic oligozoospermia.

关键词： Infertility Cytochrome P450 oligozoospermia Tamoxifen Testosterone

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Frequency of Y chromosome microdeletions and chromosomal abnormalities in infertile Thai men with oligozoospermia and azoospermia

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Asian Journal of Andrology 2007年第1期9卷 68-75页

作者： Terapom Vutyavanich Warapom Piromlertamom Wasna Sirirungsi Supachai Sirisukkasem Division of Reproductive Medicine Department of Obstetrics and Gynecology Faculty of Medicine Chiang Mai University Chiang Mai 50200 Thailand Division of Clinical Microbiology Department of Medical Technology Faculty of Associated Medical Sciences Chiang Mai University Chiang Mai 50200 Thailand

Aim： To investigate the possible causes of oligozoospermia and azoospermia in infertile Thai men, and to find the frequencies of Y chromosome microdeletions and cytogenetic abnormalities in this group. Methods： From June 2003 to November 2005, 50 azoospermic and 80 oligozoospermic men were enrolled in the study. A detailed history was taken for each man, followed by general and genital examinations. Y chromosome microdeletions were detected by multiplex polymerase chain reaction （PCR） using 11 gene-specific primers that covered all three regions of the azoospermic factor （AZFa, AZFb and AZFc）. Fifty men with normal semen analysis were also studied. Karyotyping was done with the standard G- and Q-banding. Serum concentrations of follicle stimulating hormone （FSH）, luteinizing hormone （LH）, prolactin （PRL） and testosterone were measured by electrochemiluminescence immunoassays （ECLIA）. Results： Azoospermia and oligozoospermia could be explained by previous orchitis in 22.3%, former bilateral cryptorchidism in 19.2%, abnormal karyotypes in 4.6% and Y chromosome microdeletions in 3.8% of the subjects. The most frequent deletions were in the AZFc region （50%）, followed by AZFb （33%） and AZFbc （17%）. No significant difference was detected in hormonal profiles of infertile men, with or without microdeletions. Conclusion： The frequencies of Y chromosome microdeletions and cytogenetic abnormalities in oligozoospermic and azoospermic Thai men are comparable with similarly infertile men from other Asian and Western countries.

关键词： azoospermia factor azoospermia male infertility oligozoospermia Y chromosome microdeletions

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Differential expression of VASA gene in ejaculated spermatozoa from normozoospermic men and patients with oligozoospermia

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Asian Journal of Andrology 2007年第3期9卷 339-344页

作者： Xin Guo Yao-Ting Gui Ai-Fa Tang Li-Hua Lu Xin Gao Zhi-Ming Cai The Laboratory of Male Reproductive Medicine Peking University Shenzhen Hospital Shenzhen 518036 China Department of Urosurgery The Third Affiliated Hospital Sun Yet-Sen University Guangzhou 510630 China

Aim： To detect the expression of VASA in human ejaculated spermatozoa, and to compare the expression of VASA between normozoospermic men and patients with oligozoospermia. Methods： Ejaculated spermatozoa were collected from normozoospermic men and patients with oligozoospermia by masturbation, and subsequently segregated through a discontinuous gradient of Percoll to obtain the spermatozoa. Reverse transcription polymerase chain reaction （RT- PCR）, quantitative RT-PCR （QRT-PCR）, immunoflurescence and Western blotting were used to detect the expression of VASA in mRNA and protein levels. Results： VASA mRNA was expressed in the ejaculated spermatozoa. QRT-PCR analysis showed that VASA mRNA level was approximately 5-fold higher in normozoospermic men than that in oligozoospermic men. Immunofluorescence and Western blotting analysis showed that VASA protein was located on the cytoplasmic membrane of heads and tails of spermatozoa, and its expression was significantly decreased in oligozoospermic men, which is similar to the result of QRT-PCR. Conclusion： The expression of VASA mRNA and protein was significantly decreased in the sperm of oligozoospermic men, which suggested the lower expression of the VASA gene might be associated with pathogenesis in some subtypes of male infertility and VASA could be used as a molecular marker for the diagnosis of male infertility.

关键词： VASA ejaculated spermatozoa oligozoospermia male infertility spermatogenesis

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Gonadotropin and Testosterone hormone’s serum levels and partial deletions in the AZFc region in Iranian oligozoospermia infertile males

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Health 2011年第9期3卷 566-570页

作者： Nasser Salsabili Reza Mirfakhraei Maryam Montazeri Mitra Ataei Paricheher Yaghmaei Gholamreza Pourmand IVF Department of Mirza Kouchak Khan Hospital Rehabilitation Faculty Tehran University of Medical Sciences Department of Biology Islamic Azad University (IAU) Science and Research Branch Department of Medical Genetic National Institute of Genetic E

To investigate the relation of luteinizing hormone (LH), follicle-stimulating hormone (FSH) and Testosterone serum levels with partial deletions in the AZFc region in Iranian oligozoospermia males. Material and methods: thirty infertile oligozoospermia and 52 Iranian fertile men included. The hormonal assays were measured by the Radioimmunoassay (RIA). Multiplex polymerase chain reaction (M-PCR) using eight sequence-tagged site (STS) markers were measured on the Yq11 chromosome. Results: The mean of FSH and LH levels in all oligozoospermia males were higher than fertile men (p oligozoospermia males, hormonal assay and molecular screening should be advised before considering for ART treatments.

关键词： oligozoospermia Gonadotropin Hormone AZFc Partial Deletion

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Effectiveness of Combined Testosterone Undecanoate with Tamoxifen Citrate Treatment in Men with Idiopathic Azoospermia or Serious oligozoospermia

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Journal of Reproduction and Contraception 2012年第4期23卷 254-258页

作者： Bo SONG Wei-ping QIAN Guangdong Key Lab. Male Reproductive Medicine & Genetics Reproductive Medicine Department Shenzhen Hospital Peking University

Objective To assess the effect of treatment with a combination of the tamoxifen citrate and testosterone undecanoate on sperm variables in men with idiopathic azoospermia or oIigozoospermia. Methods Four men with idiopathic azoospermia and 8 men with idiopathic oligozoospermia were collected in this retrospective and self control trial. All patients were treated with testosterone undecanoate of 80 mg/d and tamoxifen citrate of 20 mg/d. Sperm characteristics and pregnancy incidence were measured every month during 4 months medication. Results Tamoxifen citrate plus testosterone undecanoate treatment produced a satisfactory improvement of total sperm number, motility after 2 and 3 months whereas serum FSH, LH levels increased after 2 or 3 months. Conclusion The combination of tamoxifen citrate with testosterone undecanoate could improve significantly sperm count. The combination could be used in men with idiopathic azoospermia or serious oligozoospermia.

关键词： testosterone tamoxifen citrate oligozoospermia azoospermia

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Genetic polymorphisms of CYP2D6*10 and the assessment of effects treated with oral tamoxifen citrate and testosterone undecanoate in infertile men with idiopathic oligozoospermia

Genetic polymorphisms of CYP2D6*10 and the assessment of eff...

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2013年贵州省泌尿外科学术会议

作者： Kaifa Tang Shaoyuan Xu Ruizhe Liu Xinyang Wang Jiaqi Shi Fa Sun Junping Xing Department of Urology Affiliated Hospital of Guiyang Medical College Department of Urology First Affiliated Hospital of Medical College of Xi'an Jiaotong University

Objectives:To investigate the genetic polymorphisms of cytochrome P4502D6*10(CYP2D6*10),and to assess the effects of treatment with tamoxifen citrate and testosterone undecanoate on serum sex hormones,sperm variables and pregnancy incidence in infertile men with idiopathic ***:This case-control study including 204infertile men with idiopathic oligozoospermia and 147 *** patients were treated with tamoxifen citrate,20 mg/d and testosterone undecanoate,40mg/d for 6 *** polymorphisms of CYP2D6*10,sperm parameters,sex hormones,and incidence of spontaneous pregnancy were detected at starting,3-month,and 6-months,***:No significant differences between the control group and patient group with respect to the frequencies of the CYP2D6*10 C/C,CYP2D6*10 C/T,or CYP2D6*10 T/T genotypes in this study(P=0.707,OR=0.902;P=0.985,OR=1.004;P=0.751,OR=1.082).At the 3-Month evaluation,the FSH,LH,and T level in C/C subgroup was higher than these in C/T subgroup or T/T subgroup,respectively(P<0.001,P=0.002;P=0.009,P=0.001;P<0.001,P<0.001).The sperm density in C/C group was higher than this in T/T subgroup(P=0.004);and the motility in C/C subgroup was higher than this in C/T subgroup or T/T subgroup,respectively(P=0.009,P=0.021).At 6-Month evaluation,the FSH,LH,and T level in C/C subgroup was higher than these in C/T subgroups or T/T subgroup,respectively(P<0.001,P<0.001;P<0.001,P<0.001;P<0.001,P<0.001).The sperm density,motility,and normal morphology in C/C subgroup was higher than this in C/T subgroup or T/T subgroup,respectively(P<0.001,P<0.001;(P<0.001,P<0.001;P=0.002,P<0.001).In addition,there was shown that the incidence of spontaneous pregnancy in C/Csubgroup was higher than this in C/T subgroup or T/T,respectively(x=6.152,P=0.013;x=12.198,P<0.001).Conclusions:We found that CYP2D6*10 genotype affects the efficacy of tamoxifen citrate combined with testosterone undecanoate treatment in infertile men with idiopathic

关键词： Infertility oligozoospermia Tamoxifen Testosterone cytochrome P450

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Effectiveness of combined testosterone undecanoate with tamoxifen citrate treatment in men with idiopathic azoospermia or serious oligozoospermia

Effectiveness of combined testosterone undecanoate with tamo...

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中华医学会生殖医学分会人类精子库管理学组第三届年会全国男性生殖医学和精子库管理新进展第四次研讨会

作者： Bo song Zhi-ming Cai Weiping Qian Guangdong Key Lab.Male Reproductive Medicine & Genetics Shenzhen HospitalPeking University

Objective:To assess the effect of treatment with a combination of the tamoxifen citrate and testosterone undecanoate on sperm variables in men with idiopathic azoospermia or ***:Retrospective,self control ***:four men with idiopathic azoospermia and eight men with idiopathic oligozoospermia Interventions:All patients were treated with testosterone undecanoate,80mg/d and tamoxifen citrate,20mg/*** counseling was part of the intervention in all *** Outcome Measure(s):Sperm characteristics and pregnancy incidence every month after 1 and 4 months medication. Result(s):Tamoxifen citrate plus T undecanoate treatment produced a satisfactory improvement of total sperm number,motility after 2 and 3 months whereas FSH,LH in serum increased after ***:These results indicate that the combination of tamoxifen citrate with T undecanoate could improve significantly sperm *** combination deserves a place as a first line of treatment in spermatogenic arrest.

关键词： Testosterone tamoxifen citrate oligozoospermia azoospermia spermatogenic arrest

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AZF deletion in patients with azoospermia and oligozoospermia

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Asian Journal of Andrology 2003年第1期 64-64页

作者： Xian-Ning ZHANG, Zhi-Hong HU, Feng-Gen CAO, Ji ZUO Department of Genetics, School of Medicine, Ningbo University,Ningbo 315211, ChinaDepartment of Medical Genetics, Fudan University School of Medicine, Shanghai 200032, China

Objective: To investigate the relationship between AZF deletion and azoospermia/ oligozoospermia. Methods: Multiplex PCR were use to detect the deletions of 4 STSs located at the AZF region, namely sY86 at AZFa, sY127 at AZFb and sY254 and sY255 at AZFc. PCR-SSCP was used to check the sY254 point mutations in 84 Chinese patients with azoospermia or oligozoospermia. The expression pattern of DAZ gene in different tissues was studied using the RT-PCR method. Results: The concurrent deletions of sY254 and sY255 in AZFc subregion were found in 3 azoospermia patients. There were no sY254 point mutation in 48 patients studied. DAZ gene was only expressed in the testicular tissue. Conclusion: AZFc deletions may be associated with azoospermia.

关键词： AZF DAZ deletion azoospermia oligozoospermia

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