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Heterogeneous expression of stearoyl-acyl carrier protein desaturase genes SAD1 and SAD2 from Linum usitatissimum enhances seed oleic acid accumulation and seedling cold and drought tolerance in Brassica napus

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Journal of Integrative Agriculture 2024年第6期23卷 1864-1878页

作者： Jianjun Wang Yanan Shao Xin Yang Chi Zhang Yuan Guo Zijin Liu Mingxun Chen State Key Laboratory of Crop Stress Biology for Arid Areas/National Yangling Agricultural Biotechnology&Breeding Center/Shaanxi Key Laboratory of Crop Heterosis/College of Agronomy Northwest A&F UniversityYangling 712100China

Flax(Linum usitatissimum L.)is a versatile crop and its seeds are a major source of unsaturated fatty ***-acyl carrier protein desaturase(SAD)is a dehydrogenase enzyme that plays a key role in oleic acid biosynthesis as well as responses to biotic and abiotic ***,the function of SAD orthologs from *** has not been ***,we found that two LuSAD genes,LuSAD1 and LuSAD2,are present in the genome of *** cultivar‘Longya 10’.Heterogeneous expression of either LuSAD1 or LuSAD2 in Arabidopsis thaliana resulted in higher contents of total fatty acids and oleic acid in the ***,ectopic expression of LuSAD2 in *** caused altered plant ***,the overexpression of either LuSAD1 or LuSAD2 in Brassica napus also resulted in increased contents of total fatty acids and oleic acid in the ***,we demonstrated that either LuSAD1 or LuSAD2 enhances seedling resistance to cold and drought stresses by improving antioxidant enzyme activity and nonenzymatic antioxidant levels,as well as reducing membrane *** findings not only broaden our knowledge of the LuSAD functions in plants,but also offer promising targets for improving the quantity and quality of oil,and the abiotic stress tolerance of oil-producing crops,through molecular manipulation.

关键词： LuSAD oleic acid cold tolerance drought tolerance Linum usitatissimum Brassica napus

oleic acid reduces steroidogenesis by changing the lipid type stored in lipid droplets of ovarian granulosa cells

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Journal of Animal Science and Biotechnology 2022年第3期13卷 756-768页

作者： Xiaoge Zhou Zhaoyi Mo Yankun Li Liang Huang Sihai Yu Lan Ge Yamei Hu Shengjie Shi Lutong Zhang Liguang Wang Lei Gao Gongshe Yang Guiyan Chu Key Laboratory of Animal Genetics Breeding and Reproduction of Shaanxi ProvinceYangling 712100China Laboratory of Animal Fat Deposition&Muscle Development College of Animal Science and TechnologyNorthwest A&F UniversityYangling 712100China College of Animal Science and Technology Northwest A&F UniversityYangling 712100China College of veterinary medicine Northwest A&F UniversityYangling 712100China

Background:oleic acid is an abundant free fatty acid present in livestock that are in a negative energy-balance state,and it may have detrimental effects on female reproduction and *** acid induces lipid accumulation in bovine granulosa cells,which leads to a foam cell-like morphology and reduced ***,why oleic acid increases lipid accumulation but decreases steroidogenesis remains *** study focused on oleic acid’s effects on lipid type and ***:oleic acid increased the lipid accumulation in a concentration-dependent manner and mainly increased the triglyceride level and decreased the cholesterol ester *** acid also led to a decline in estradiol and progesterone production in porcine granulosa cells in *** addition,oleic acid up-regulated the expression of CD36 and diacylglycerol acyltransferase 2,but down-regulated the expression of 3-hydroxy-3-methylglutarylcoenzyme A reductase,scavenger receptor class B member 1 and acetyl-Coenzyme A acetyltransferase 2,as well as steroidogenesis-related genes,including cytochrome P450 family 11 subfamily A member 1,cytochrome P450family 19 subfamily A member 1 and 3 as well as steroidogenic acute regulatory protein at the mRNA and protein *** oleic acid-rich diet also enhanced the triglyceride levels and reduced the cholesterol levels in ovarian tissues of female mice,which resulted in lower estradiol levels than in control-fed *** with the control,decreases in estrus days and the numbers of antral follicles and corpora lutea,as well as an increase in the numbers of the atretic follicles,were found in the oleic acid-fed female ***:oleic acid changed the lipid type stored in lipid droplets of ovarian granulosa cells,and led to a decrease in *** results improve our understanding of fertility decline in livestock that are in a negative energy-balance state.

关键词： Fertility Granulosa cell Lipid droplet oleic acid Steroidogenesis

Developmental changes in lipid and fatty acid metabolism and the inhibition by in ovo feeding oleic acid in Muscovy duck embryogenesis

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Animal Nutrition 2023年第1期12卷 321-333页

作者： Xiufen Zhang Qilin Wu Wenxuan Zheng Chuang Liu Liang Huang Xin Zuo Wenquan Xiao Xiaofeng Han Hui Ye Wence Wang Lin Yang Yongwen Zhu Guangdong Provincial Key Laboratory of Animal Nutrition and Regulation College of Animal ScienceSouth China Agricultural UniversityGuangzhou 510642China Wen's Food Group Co. LtdYunfu 52740China

Hepatic lipid and fatty acid(FA)metabolism are critical for regulating energetic homeostasis during *** present,it remains unclear how an exogenous FA intervention affects embryonic development in an avian embryo *** Exp.1,30 fertilized eggs were sampled on embryonic days(E)16,19,22,25,28,31 and the day of hatch(DOH)to determine the critical period of lipid *** Exp.2,a total of 120 fertilized eggs were divided into two groups(60 eggs/group)for in ovo feeding(IOF)procedures on *** were injected into the yolk sac with PBS as the control group and with oleic acid(OA)as the IOF-OA treatment *** were collected on E28 and *** Exp.1,hepatic triacylglycerol(TG)and cholesterol(CHO)contents increased while serum TG content decreased from E16 to DOH(P0.05).Maximal metabolic changes in lipid and FA metabolism occurred on E22-E28 in Muscovy duck embryogenesis,along with the altered target gene and protein expression related to lipogenesis and ***-OA intervention on E25 could inhibit the target gene expression related to FA uptake,synthesis,and oxidation,which may influence the normal FA metabolism on E28 during embryogenesis.

关键词： Embryo Lipid Triacylglycerol Cholesterol Fatty acid oleic acid

The protective effect of oleic acid on Lactiplantibacillus plantarum during freeze-drying

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Food Science and Human Wellness 2023年第6期12卷 2355-2361页

作者： Yongjun Xia Yizhen Wang Jing Pu Yan Wu Zhiqiang Xiong Xin Song Hui Zhang Lianzhong Ai Guangqiang Wang Shanghai Engineering Research Center of Food Microbiology School of Health Science and EngineeringUniversity of Shanghai for Science and TechnologyShanghai 200093China Department of Food Science&Technology School of Agriculture and BiologyShanghai Jiao Tong UniversityShanghai 200240China

Membrane characteristics are determined by the fatty acids composition,which affects survival rates after ***,it is unknown which composition provides the greatest *** this study,we found that the addition of Tween 80 and Tween 20 significantly increased survival rates of Lactiplantibacillus plantarum,which reached a maximum of 93.1%.Conversely,Tween 60 caused a significant *** further found that the difference between the effects of adding Tween 80 and Tween 60 was the change in oleic acid *** verify the role of oleic acid,we used CRISPR-Cas9 to knock-out the key synthesis gene *** survival rates of *** AR113Δcla-er declined to 5.48%.The addition of oleic acid restored the rates to those of wild type ***,the membrane integrity and fl uidity of knockout strains markedly *** is the fi rst confi rmation that Tween 80 or Tween 20 increases the survival rate by increasing the content of oleic acid in the cell *** fi ndings also indicated that oleic acid in cell membranes has a substantial protective effect on *** during freeze-drying.

关键词： Freeze-drying oleic acid Gene Knockout Lactiplantibacillus plantarum

Bioinformatics and Functional Analysis of High oleic acid-Related Gene GmSAM22 in Soybean [Glycine max (L.) Merr.]

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Phyton-International Journal of Experimental Botany 2023年第2期92卷 501-519页

作者： Shuo Qu Qi Cai Huimin Cui Lamboro Abraham Yaolei Jiao Guilong Ma Piwu Wang College of Agronomy Jilin Agricultural UniversityChangchun1300118China College of Plant Protection Agricultural UniversityChangchun1300118China

High yield,high quality,stable yield,adaptability to growth period,and modern mechanization are the basic requirements for crops in the 21st *** oleic acid is a natural unsaturated fatty acid with strong antioxidant properties and *** as a safe fatty acid,it has the ability to successfully prevent cardiovascular and cerebrovascular *** the fatty acid composition of soybean seeds,can not only speed up the breeding process of high-quality high-oil and high-oleic soybeans,but also have important significance in human health,and provide the possibility for the development of soybean oil as a new energy ***,the aim of this study was to analyze the high oleic acid elated gene GmSAM22 in *** this research the soybean oleic acid-related gene GmSAM22 was screened out by Genome-wide association analysis,a 662 bp fragment was acquired by specific PCR amplification,and the pMD18T cloning vector was linked by the use of a seamless cloning *** analysis of the signal peptide prediction,subcellular localization,protein hydrophobicity,transmembrane region analysis,a phosphorylation site,protein secondary and tertiary structure and protein interaction analysis of the protein encoded by the SAM22 gene was carried *** plasmid of the gene editing vector is *** overexpression vector was transformed from pCAMBIA3301 as the base vector,and overexpression vector were *** plants were obtained by genetic transformation by the pollen tube channel *** quantitative PCR was performed on the T2 generation plants to detect the relative expression levels in different *** Blot was used to detect the presence of hybridization *** genes BAR,35S,and NOS in plants were identified by conventional PCR.10 seeds with high and low oleic acid content were chosen for quantitative PCR identification,and finally,the concentration and morphology of soybean fatty acids were

关键词： Soybean SAM22 gene oleic acid cloning CRISPR/Cas9 expression vector

oleic acid promotes atherosclerosis via multiple pathophysiological mechanisms:a narrative review

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Journal of Bio-X Research 2022年第2期5卷 64-66页

作者： Ting Chu Maosheng Yang Department of Nursing School of NursingZhejiang Chinese Medical University HangzhouZhejiang Province Laboratory of Disorders Genes and Department of Pharmacology Jishou University School of PharmacyJishouHunan ProvinceChina

An increasing body of evidence supports the hypothesis that atherosclerosis is caused by multiple factors and mechanisms.A better understanding of the contribution of hypertriglyceridemia or oleic acid to the pathogenesis of atherosclerosis would significantly increase our ability to successfully treat these *** propose that oleic acid promotes atherosclerosis via multiple pathophysiological *** we suggest that(1)atherosclerosis may develop through multiple pathophysiological mechanisms and that this may be a common feature of human diseases;(2)diseases may be the consequence of natural selection or the interaction of internal and external factors;(3)diseases should be recognized with“biological diversity,”and that this concept should be introduced into medical education,clinical treatment,drug research and development,and medical *** work reported here will benefit a new generation of medical trainees and promote the development of the relevant medical fields.

关键词： atherosclerosis biological diversity cardiovascular disease hypertriglyceridemia oleic acid

oleic acid (OA), A Potential Dual Contrast Agent for Postmortem MR Angiography (PMMRA): A Pilot Study

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Current Medical Science 2020年第4期40卷 786-794页

作者： Zhi-yuan XIA Christine BRUGUIER Fabrice DEDOUIT Silke GRABHERR Marc AUGSBURGER Bei-bei LIU Institute of Evidence Law and Forensic Science China University of Political Science and Law(CUPL)Key Laboratory of Evidence Law and Forensic ScienceMinistry of EducationBeijing 100088China University Center of Legal Medicine Lausanne-Geneva(CURML)Lausanne CH1000Switzerland Service de Mede cine Legale Hopital de RangueilToulouse 50032France Dian Research Center for Postmortem Imaging&Angiography Beijing 100192China

Summary:Choosing proper perfusates as contrast agents is an important aspect for postmortem magnetic resonance angiography(PMMRA).However,in this emerging field,the number of suitable kinds of liquid is still very *** objective of this research is to compare MR images of oleic acid(OA)with paraffin oil(PO)in vitro and in ex situ animal hearts,in order to evaluate the feasibility to use OA as a novel contrast agent for *** vitro,OA,PO and water(control)were introduced into three tubes separately and T,weighted-spin echo(Tw SE)and T2w-SE images were acquired on a 1.5T MR *** the second experiment,0A and PO were injected into left coronary artery(LCA)and left ventricle(LV)of ex situ bovine hearts and their Tw-SE,Tzw-SE,Tw-multipoint Dixon(Tjw-mDixon)and 3DT2w-mDixon images were *** overall results indicate that OA may have a potential to be used as a dual(T and T2 based)contrast agent for PMMRA when proper sequence parameters are ***,as the pilot study was based on limited number of animal hearts,more researches using OA in cadavers are needed to validate our findings.

关键词： postmortem magnetic resonance angiography perfusate contrast agent oleic acid paraffin oil heart

Influence of surface modification of SrFe12O19 particles with oleic acid on magnetic microsphere preparation

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Particuology 2008年第3期6卷 185-190页

作者： Sifang Kong Peipei Zhang Xiufang Wen Pihui Pi Jiang Cheng Zhuoru Yang Jing Hai School of Chemical and Energy Engineering South China University of Technology Guangzhou 501640 China South China Institute of Environmental Sciences SEPA Guangzhou 510655 China

oleic acid was used as surface modification agent to improve the hydrophobicity of magnetic strontium hexaferrite particles. The structure and properties of treated magnetic particles were characterized by scanning electronic microscopy （SEM）, Fourier transform infrared spectra （FTIR）, powder X-ray diffraction （XRD） and magnetic property measurement system （MPMS）. The results show that oleic acid is chemically enwrapped on the surface of SrFe12O19 particles. Magnetic particles modified by oleic acid are highly dispersible and strongly responsive to magnetism but with slight decrease in saturated magnetization. The affinity between magnetic particles and monomers is improved by surface modification, resulting in increased particle incorporation in magnetic polymeric microspheres. The surface modification mechanism of magnetic particles by oleic acid is addressed in this work.

关键词： oleic acid Surface modification mechanism Strontium hexaferrite Dispersibility Magnetic polymeric microsphere

Synergistic mechanism between SDBS and oleic acid in anionic flotation of rhodochrosite

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International Journal of Minerals,Metallurgy and Materials 2015年第5期22卷 447-452页

作者： Yong-jie Bu Run-qing Liu Wei Sun Yue-hua Hu School of Minerals Processing and Bioengineering Central South University

Pure mineral flotation experiments, zeta potential testing, and infrared spectroscopy were employed to investigate the interracial reactions of oleic acid （collector）, sodium dodecyl benzene sulfonate （SDBS, synergist）, and rhodochrosite in an anionic system. The pure mineral test shows that oleic acid has a strong ability to collect products on rhodochrosite. Under neutral to moderately alkaline conditions, low temperature （e.g., 10℃） adversely affects the flotation performance of oleic acid; the addition of SDBS significantly improves the dis- persion and solubility of oleic acid, enhancing its collecting ability and flotation recovery. The zeta potential test shows that rhodochrosite interacts with oleic acid and SDBS, resulting in a more negative zeta potential and the co-adsorption of the collector and synergist at the mineral surface. Infrared spectroscopy demonstrated that when oleic acid and SDBS are used as a mixed collector, oleates along with -COO- and --COOH functional groups are formed on the mineral surface, indicating chemical adsorption on rhodochrosite. The results demonstrate that oleic acid and SDBS co-adsorb chemically on the surface ofrhodochrosite, thereby improving the flotation performance of the collector.

关键词： rhodochrosite flotation sodium dodecyl benzene sulfonate oleic acid synergistic effects temperature