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Smashing Tissue Extraction and GC Analysis of Active Fatty Acids from oil cake of perilla seeds

Chinese Herbal Medicines 2011年第1期3卷 75-78页

作者： SUN Yan-ling1,LIU Yan-ze2,3,XIAO Han4,WEI Ying-feng4,ZHAO Yu-qing1,5 1.Shenyang Pharmaceutical University,Shenyang 110006,China 2.Bio-organic and Natural Product Laboratory,MRC 311,McLean Hospital/Harvard Medical School,Belmont,MA 02478,USA 3.Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences,Beijing 100193,China 4.Liaoning Jiashi Nutritional Plant oil Development Co.Ltd.,Shenyang 110035,China 5.Key Laboratory of Structure-Based Drug Design&Discovery,Shenyang Pharmaceutical University,Ministry of Education,Shenyang 110016,China Shenyang Pharmaceutical University Shenyang 110006 China Bio-organic and Natural Product Laboratory MRC 311 McLean Hospital/Harvard Medical School Belmont MA 02478 USA Institute of Medicinal Plant Development Chinese Academy of Medical Sciences Beijing 100193 China Liaoning Jiashi Nutritional Plant oil Development Co. Ltd Shenyang 110035 China Shenyang Pharmaceutical University Shenyang 110006 China Key Laboratory of Structure-Based Drug Design&Discovery Shenyang Pharmaceutical University Ministry of EducationShenyang 110016 China

Objective To optimize the extraction technology of perilla seeds oil from the oil cake of perilla seeds(OCPS)by using the contents of active fatty acids as evaluation *** The fatty acids were extracted from OCPS,the residue of perilla seeds after cold-press,by smashing tissue extraction(STE),the new technology selected through comparing with classical leaching extraction(LE),Soxhlet extraction(SE),ultrasonic extraction(UE),and supercritical-CO2 fluid extraction(SFE).For optimized condition of STE,orthogonal test was designed and *** contents of five fatty acids in extracted oil and OCPS were determined by *** The optimized extraction parameters were smashing for 1.5 min under extraction power of 150 W and 1:6 of the material/solvent *** contents of five fatty acids in the oils extracted by five techniques from OCPS and determined by GC were as follows:α-linolenic acid(41.12%-51.81%),linoleic acid(15.38%-16.43%),oleic acid (18.93%-27.28%),stearic acid(2.56%-4.01%),and palmitic acid(7.38%-10.77%).Conclusion The results show that STE is the most efficient technology with the highest yield(LE:0.57%;SE:1.03%;UE:0.61%;SFE:0.80%;STE: 1.17%)and shortest time(LE:720 min;SE:360 min;UE:30 min;SFE:120 min;STE:1.5 min)among five tested extraction *** is first reported using STE to extract herbal oil enriched with active fatty acids.

关键词： fatty acids flash extraction GC analysis oil cake of perilla seeds perilla frutescens smashing tissue extraction

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