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Analytical characterization and structure elucidation of metabolites from Aspergillus ochraceus MP2 fungi

Asian Pacific Journal of Tropical Biomedicine 2011年第5期1卷 376-380页

作者： Meenupriya J Thangaraj M Department of Biotechnology Sathyabama University CAS in Marine biology Annamalai university

Objective:To isolate and characterize the bioactive secondary metabolites from Aspergillus ochraceus(***)MP2 ***:The anti bacterial activity of marine sponge derived fungi *** MP2 was thoroughly investigated against antagonistic human *** optimum inhibitory concentration of the fungi in the elite solvent was also *** promising extracts that showed good antimicrobial activity were subjected to further analytical separation to get individual distinct metabolites and the eluants were further identified by GC MS instrumental *** molecular characterization of the elite fungal strains were done by isolating their genomic DNA and amplify the internal transcribed spacer(US)region of 5.8s rRNA using specific ITS *** novelty of the strain was proved by homology search tools and elite sequences was submitted to ***:Three bioactive compounds were characterized to reveal their identity,chemical formula and *** first elutant was identified asα-Campholene aldehyde with chemical fonnula C_(10)H_(16)O and molecular weight 152 *** second elutant was identified as Lucenin-2 and chemical fonnula C_(27)H_(30)O_(16)and molecular weight *** third elutant was identified as 6-Ethyloct-3-yl-2-ethylhexyl ester with Chemical fonnula C_(26)H_(42)O_4 with molecular weight 418 ***:The isolated compounds showed significant antimicrobial activity against potential human *** secondary metabolites represent a large source of compounds endowed with ingenious structures and potent biological activities.

关键词： Marine sponge derived fungi Aspergillus ochraceus Bioactive metabolites Antibacterial activity Analytical characterization Fungi Apergillus Bioactive secondary metabolite Antagonistic human pathogen Antimicrobial activity Bioactive compounds

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