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Non-Protein-Coding RNAs and their Interacting RNA-Binding Proteins in the Plant Cell nucleus

Molecular Plant 2010年第4期3卷 729-739页

作者： Celine Charon Ana Beatriz Moreno Florian Bardou Martin Crespi University Paris-Sud Institut de Biologie des Plantes UMR8618 Orsay Cedex F-91405 France CNRS Orsay Cedex F-91405 France Institut des Sciences du Vegetal CNRS F-91198 Gif-sur-Yvette Cedex France

The complex responses of eukaryotic cells to external factors are governed by several transcriptional and post-transcriptional processes. Several of them occur in the nucleus.and have been linked to the action of non-proteincoding RNAs （or npcRNAs）, both long and small npcRNAs, that recently emerged as major regulators of gene expression. Regulatory npcRNAs acting in the nucleus.include silencing-related RNAs, intergenic npcRNAs, natural antisense RNAs, and other aberrant RNAs resulting from the interplay between global transcription and RNA processing activities （such as Dicers and RNA-dependent polymerases）. Generally, the resulting npcRNAs exert their regulatory effects through interactions with RNA-binding proteins （or RBPs） within ribonucleoprotein particles （or RNPs）. A large group of RBPs are implicated in the silencing machinery through small interfering RNAs （siRNAs） and their localization suggests that several act in the nucleus.to trigger epigenetic and chromatin changes at a whole-genome scale. Other nuclear RBPs interact with npcRNAs and change their localization. In the fission yeast, the RNA-binding Mei2p protein, playing pivotal roles in meiosis, interact with a meiotic npcRNA involved in its nuclear re-localization. Related processes have been identified in plants and the ENOD40 npcRNA was shown to re-localize a nuclear-speckle RBP from the nucleus.to the cytoplasm in Medicago truncatula. Plant RBPs have been also implicated in RNA-mediated chromatin silencing in the FLC locus through interaction with specific antisense transcripts. In this review, we discuss the interactions between RBPs and npcRNAs in the context of nuclear-related processes and their implication in plant development and stress responses. We propose that these interactions may add a regulatory layer that modulates the interactions between the nuclear genome and the environment and, consequently, control plant developmental plasticity.

关键词： Abiotic/environmental stress gene silencing Arabidopsis RNA-binding proteins non-protein-coding RNAs nucleus.

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Probing proton halo of the exotic nucleus.^(28)S by elastic electron scattering

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Science China(Physics,Mechanics & Astronomy) 2004年第1期47卷 42-51页

作者： WANG Zaijun1 & REN Zhongzhou1’2 1. Department of Physics, Nanjing University, Nanjing 210008, China 2. Center of Theoretical Nuclear Physics, National Laboratory of Heavy-Ion Accelerator at Lanzhou, Lanzhou 730000, China Correspondence should be addressed to Ren Zhongzhou (email: zren@*** zren99@***) Received June 16, 2003 Department of Physics Nanjing University Nanjing 210008 China Center of Theoretical Nuclear Physics National Laboratory of Heavy-Ion Accelerator at Lanzhou Lanzhou 730000 China

Elastic electron scattering on the exotic light nucleus.28S is investigated in the plane wave Born approximation. The variation of the squared form factors of 28S with momentum transfer is compared with that of 32S. It is found that the behavior of the form factors near the second minimum (with a moderate momentum transfer) is sensitive to the alteration of the charge density distribution of halo protons in 28S. This indicates that elastic electron scattering can be a good probe of the structure of proton-halo nuclei.

关键词： elastic electron scattering, cross section, form factor, proton-halo nucleus.

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Microsatellite DNA analysis proves nucleus.of interspecies reconstructed blastocyst coming from that of donor giant panda

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Chinese Science Bulletin 2000年第20期45卷 1883-1885页

作者： DING Bo SHI Peng XIANGYU Jinggong ZHANG Yaping CHEN Dayuan SUN Qingyuan LI Guangpeng WANG Minkang LIU Yilong KANG Li HAN Zhiming SONG Xiangfen LI Jingsong CHEN Yuchun Laboratory of Cellular and Molecular Evolution Kunming Institute of ZoologyChinese Academy of SciencesKunming 650223China State Key Laboratory of Reproductive Biology Institute of ZoologyChinese Academy of SciencesBeijing 100080China Fuzhou Panda Institute Center Fuzhou 350001China 不详

A method for DNA isolation from early development of blastocyst and further analysis of nuclear and mitochondrial DNA was developed in present study. Total DNA was prepared from interspecies reconstructed blastocyst and a giant panda specific microsatellite locus g010 was successfully amplified. DNA sequencing of the PCR product showed that two sequences of reconstructed blastocysts are the same as that of positive control giant panda. Our results prove that the nucleus.of interspecies reconstructed blastocyst comes from somatic nucleus.of donor giant panda.

关键词： giant panda (Ailuropoda melanoleuca) interspecies cloning microsatellite DNA nucleus.

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Completely regular paracompact reflection of locales

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Science China Mathematics 2006年第6期49卷 820-826页

作者： HE Wei & LUO Maokang Department of Mathematics, Nanjing Normal University, Nanjing 210097, China Department of Mathematics, Sichuan University, Chengdu 610064, China Department of Mathematics Nanjing Normal University Nanjing China Department of Mathematics Sichuan University Chengdu China

We give an explicit construction of the completely regular paracompact reflection pL of a completely regular locale L described as a sublocale of the Stone-Cech compactification βL of L.

关键词： completely regular paracompact locale, nucleus.

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Behavior of 280-kD Nuclear Matrix Protein in Mitotic HeLa Ceils

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Chinese Science Bulletin 1994年第19期39卷 1656-1658页

作者：蔡树涛翟中和 Department of Biology Pekiny University Beijing 100871. PRC

Nuclear matrix is an insoluble proteinaceous filament network in nucleus. Numerous research works demonstrated that many important life activities, such as DNA replication, RNA transcription and processing, chromatin assembly, are associated with nuclear matrix. DNA loops are reported to be anchored at the nuclear matrix filaments. So far, the detailed mechanism of the association is not very clear.

关键词： nuclear matrix mitosis nucleus.

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