检索结果-南通市图书馆

Structural components of the nuclear body in nuclei of Allium cepa cells

Cell Research 2001年第1期11卷 68-73页

作者： TAO WEI, CHANG HUI YAN, TAO CAI, SHUI HAO, ZHONG HE ZHAI (College of Life Sciences, Peking University, Beijing 100871, China) (Institute of Genetics and Cytology, Northeast Normal University, Changchun Jilin 130024, China) 1College of Life Sciences Peking University Beijing 100871 China 1College of Life Sciences Peking University Beijing 100871 China 1College of Life Sciences Peking University Beijing 100871 China 2Institute of Genetics and Cytology Northeast Normal University Changchun Jilin 130024 China 1College of Life Sciences Peking University Beijing 100871 China

nuclear bodies have long been noted in interphase nuclei of plant cells, but their structural component, origin and fUnction are still unclear by now. The present work showed in onion cells the nuclear bodies appeared as a spherical structure about 0.3 to 0.8 μm in diameter. They possibly were formed in nucleolus and subsequently released, and entered into nucleoplasm. Observation through cytochemical staining method at the ultrastructural level codeined that nuclear bodies consisted of ribonucleoproteins (RNPs) and silver-stainable proteins. Immunocytochemical results revealed that nuclear bodies contained no DNA and ribosomal gene transcription factor (UBF). Based on these data, we suggested that nuclear bodies are not related to the ribosome or other gene transcription activities, instead they may act as subnuclear structures for RNPs transport from nucleolus to cytoplasm, and may also be involved in splicing of pre-mRNAs.

关键词： nuclear body structural component ribonucleoproteins nucleolus Allium cepa.

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

The Arabidopsis CROWDED NUCLEI genes regulate seed germination by modulating degradation of ABI5 protein

引用

Journal of Integrative Plant Biology 2016年第7期58卷 669-678页

作者： Wenming Zhao Chunmei Guan Jian Feng Yan Liang Ni Zhan Jianru Zuo Bo Ren State Key Laboratory of Plant Genomics and National Plant Gene Research Center Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049china Institute of Microbiology Chinese Academy of SciencesBeijing 100101China John Innes Centre Norwich NR4 7UHUnited Kingdom Institute of Biotechnology Zhejiang UniversityHangzhou 310058China

In Arabidopsis, the phytohormone abscisic acid （ABA） plays a vital role in inhibiting seed germination and in postgermination seedling establishment. In the ABA signaling pathway, ABI5, a basic Leu zipper transcription factor, has important functions in the regulation of seed germination. ABI5 protein localizes in nuclear bodies, along with AFP, COP1, and SIZ1, and was degraded through the 26S proteasome pathway. However, the mechanisms of ABI5 nuclear body formation and ABI5 protein degradation remain obscure. In this study, we found that the Arabidopsis CROWDED NUCLEI （CRWN） proteins, predicted nuclear matrix proteins essential for maintenance of nuclear morphology, also participate in ABA-controlled seed germination by regulating the degradation of ABI5 protein. During seed germination, the crwn mutants are hypersensitive to ABA and have higher levels of ABI5 protein compared to wild type. Genetic analysis suggested that CRWNs act upstream of ABIS. The observation that CRWN3 colocalizes with ABI5 in nuclear bodies indicates that CRWNs might participate in ABI5 protein degrada- tion in nuclear bodies. Moreover, we revealed that the extreme C-terminal of CRWN3 protein is necessary for its function in the response to ABA in germination. Our results suggested important roles of CRWNs in ABI5 nuclear body organization and ABI5 protein degradation during seed germination.

关键词： ABI5 Arabidopsis CROWDED NUCLEI germination nuclear body

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Genome Organization and Function： A View from Yeast and Arabidopsis

引用

Molecular Plant 2010年第4期3卷 678-690页

作者： Julio Saez-Vasquez Olivier Gadal LGDP-UMR 5096 CNRS-IRD-Universite de Perpignan via Dornitia 58 Av. Paul Alduy 66860 Perpignan France Laboratoire de Biologie Moleculaire Eucaryote Universite de Toulouse Toulouse France LBME CNRS Toulouse France

Recent progress in understanding higher-order chromatin organization in the nucleus has been considerable. From single gene to chromosome territory, realistic biophysical models can now accurately predict some of the structural feature of cell nuclei. Despite growing evidence of a deterministic nuclear organization, the physiological consequence of spatial genome organization is still unclear. In the simple eukaryotic model, Saccharomyces cerevisiae, clear correlation between gene position and transcription has been established. In this review, we will focus on higher-order chromatin organization in yeast with respect to the nuclear envelope and nucleolus. In Arabidopsis thaliana, a model plant for which we have a complete genome sequence, chromosome territory （CT） arrangement and somatic homologous pairing in interphase nuclei seem to occur randomly. Since chromosomes containing nucleolar organizer regions associate more frequently to form a single nucleolar structure, as in yeast, the nucleolus seems to play a major role in organizing nuclear space. Recent findings have begun to elucidate how plant regulatory factors, such as chromatin remodeling or histone chaperones, affect the chromatin state of ribosomal DNA genes located in two distinct CTarrangements in the nucleus. The functional outcome of yeast nuclear organization allowed us to propose how nuclear organization might contribute to a novel type of epigenetic regulation： the spatial regulation of transcription.

关键词： nuclear structure chromatin organization nuclear body nucleolus.

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：