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nuclear magnetic resonance spectroscopy is highly sensitive for lipid-soluble metabolites

Neural Regeneration Research 2013年第22期8卷 2103-2110页

作者： Haiyang Dai Bikai Hong Zhifeng Xu Lian Ma Yaowen Chen Yeyu Xiao Renhua Wu Department of Medical Imaging Second Affiliated HospitalShantou University Medical College Department of Medical Imaging Huizhou Municipal Central Hospital Department of Radiology the First People's Hospital of Foshan Department of Pediatrics Second Affiliated HospitalShantou University Medical College Central Laboratory Shantou University

Although the water-soluble metabolite profile of human mesenchymal stem cells is known, the lipid profile still needs further investigation. In this study, methanol-chloroform was used to extract lipid-soluble metabolites and perchloric acid was used to extract water-soluble metabolites. Fur- thermore, a dual phase extraction method using methanol-chloroform and water was used to obtain both water and lipid fractions simultaneously. All metabolite extractions were analyzed on a 9.4T high-resolution nuclear magnetic resonance spectrometer. Metabolite resonance peaks were as- signed in the acquired spectra according to the chemical shift, and the extraction efficiency of dif- ferent methods was compared. Results showed that in the spectra of water-soluble extracts, major metabolites comprised low molecular weight metabolites, including lactate, acetic acid, fatty acids, threonine, glutamic acid, creatine, choline and its derivatives, while in the spectra of lipid-soluble extracts, most metabolites were assigned to fatty acids. Among the different extraction procedures, perchloric acid was more efficient in extracting water-soluble metabolites and methanol-chloroform was efficient in extracting organic components compared with the dual phase extraction method. nuclear magnetic resonance spectroscopy showed that as low as 0.7 mg organic yield was enough to obtain clear resonance peaks, while about 6.0 mg water-soluble yield was needed to obtain rela- tively favorable spectral lines. These results show that the efficiency of extracting water and lipid fractions is higher using perchloric acid and methanol-chloroform compared with dual phase ex- traction and that nuclear magnetic resonance spectroscopy is highly sensitive for analyzing lipid-soluble extracts.

关键词： neural regeneration nuclear magnetic resonance spectroscopy mesenchymal stem cells metabo-lite profiles extraction method optimization water-soluble lipid-soluble perchloric acid metha-nol-chloroform grants-supported paper neuroregeneration

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Application of nuclear magnetic resonance spectroscopy in Metabolic Differences and Potential Biomarkers Screening in Rats with Polycystic Ovary Syndrome

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Chinese Journal of Biomedical Engineering(English Edition) 2022年第4期31卷 160-167页

作者： YU Jia PAN Hai-feng WANG Han PAN Feng YANG Guang Jilin Province FAW General Hospital Changchun Jilin 130011China

Objective:To investigate the application of nuclear magnetic resonance(NMR)spectroscopy in the exploration of metabolic differences and potential biomarkers in rats with polycystic ovary syndrome(PCOS).Methods:PCOS model was established by subcutaneous injection of deoxy-epiandrosterone(DHEA)in 30 rats randomly selected from sixty 23-day-old SPF female SD rats and set as PCOS group,and the other 30 rats were subcutaneously injected with oil and set as oil group,PCOS model was successfully established by 20 days of injection in both groups,and blood samples were taken for biochemical detection and NMR detection *** the same time,the body weight,ovarian weight and HE staining results of rats in both groups were ***:Compared with the oil group,the rats in the PCOS group had higher body weight,ovarian weight and serum LH,FPG,T,E_(2)and HOMA levels,and lower serum FSH and INS levels(P<0.05).In the oil group,a large number of corpus luteum,follicles at different stages of development and multi-layered granulosa cells were observed under microscope after HE staining;In PCOS group,corpus luteum formation was only visible in some ovaries after HE staining,and all of them showed a higher number of cystic dilated follicles,thickened follicular membrane layer and less granulosa cell *** PCA score map of serum NMR profiles of the rats in the two groups showed that the samples were separated between the PCOS group and the oil group but the samples were aggregated within the group,and there were differences in blood metabolism between the PCOS model constructed by surface DHEA and the rats in the oil group.A total of 70 metabolites were detected in the serum samples of rats in the two groups by NMR technique,and 31 of them were different between the two groups,which can be classified into glucose,lipid,and and amino *** were significant differences in three metabolites,including isoleucine,valine,and creatine(P<0.01).Conclusion:There are differenc

关键词： polycystic ovary syndrome nuclear magnetic resonance spectroscopy rats metabolic differences biomarkers

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Using nuclear magnetic resonance and MS/MS spectroscopy for the Identification of Brodimoprim Metabolites in Rat Urine

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Chinese Chemical Letters 2001年第6期12卷 513-516页

作者： Chun YANG Wen Yi HE Li Jun LI Rui Ming XU Shou Ren ZHANG Zeper ABLIZ Yi Kang SI Institute of Materia Medica Chinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100050

Eight metabolites of brodimoprim (BDP) in rat urine were detected by NMR and ESIMS/MS. They were demethyl-BDP glucuronide, demethyl-BDP sulfurate, demethyl-BDP glucuronide sulfurate, alpha -hydroxyl-BDP, alpha -hydroxyl-BDP glucuronide, BDP sulfurate, N-oxide-BDP sulfurate, and alpha -hydroxyl-N-oxide-BDP sulfurate. All the sulfurates are reported for the first time.

关键词： nuclear magnetic resonance spectroscopy MS/MS spectroscopy metabolites brodimoprim

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RNA structure determination:From 2D to 3D

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Fundamental Research 2023年第5期3卷 727-737页

作者： Jie Deng Xianyang Fang Lin Huang Shanshan Li Lilei Xu Keqiong Ye Jinsong Zhang Kaiming Zhang Qiangfeng Cliff Zhang Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation Guangdong-Hong Kong Joint Laboratory for RNA MedicineSun Yat-Sen Memorial HospitalSun Yat-Sen UniversityGuangzhou 510120China Beijing Frontier Research Center for Biological Structure Center for Synthetic and Systems BiologySchool of Life SciencesTsinghua UniversityBeijing 100084China Key Laboratory of RNA Biology CAS Center for Excellence in BiomacromoleculesInstitute of BiophysicsChinese Academy of SciencesBeijing 100101China MOE Key Laboratory for Cellular Dynamics and Center for Advanced Interdisciplinary Science and Biomedicine of IHM Division of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefei 230027China University of Chinese Academy of Sciences Beijing 100049China MOE Key Laboratory of Bioinformatics Beijing Advanced Innovation Center for Structural Biology&Frontier Research Center for Biological StructureCenter for Synthetic and Systems BiologySchool of Life SciencesTsinghua UniversityBeijing 100084China Tsinghua-Peking Center for Life Sciences Beijing 100084China

RNA molecules serve a wide range of functions that are closely linked to their *** basic structural units of RNA consist of single-and double-stranded *** order to carry out advanced functions such as catalysis and ligand binding,certain types of RNAs can adopt higher-order *** analysis of RNA structures has progressed alongside advancements in structural biology techniques,but it comes with its own set of challenges and corresponding *** this review,we will discuss recent advances in RNA structure analysis techniques,including structural probing methods,X-ray crystallography,nuclear magnetic resonance,cryo-electron microscopy,and small-angle X-ray ***,a combination of multiple techniques is employed for the integrated analysis of RNA *** also survey important RNA structures that have been recently determined using various techniques.

关键词： RNA structure RNA structure probing X-ray crystallography nuclear magnetic resonance spectroscopy Cryo-electron microscopy Small angle X-ray scattering

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7.0T nuclear magnetic resonance evaluation of the amyloid beta(1–40) animal model of Alzheimer's disease: comparison of cytology verification

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Neural Regeneration Research 2014年第4期9卷 430-435页

作者： Lei Zhang Shuai Dong Guixiang Zhao Yu Ma MR Neuroradiology Room Beijing Tiantan Hospital Affiliated to Capital Medical University Department of Neurology Sixth People’s Hospital of Jinan Department of Rehabilitation Medicine Sixth People’s Hospital of Jinan Tsinghua University Yuquan Hospital

3.0T magnetic resonance spectroscopic imaging brain function in Alzheimer＇s disease. However, is a commonly used method in the research ot the role of 7.0T high-field magnetic resonance spectroscopic imaging in brain function of Alzheimer＇s disease remains unclear. In this study, 7.0T magnetic resonance spectroscopy showed that in the hippocampus of Alzheimer＇s disease rats, the N-acetylaspartate wave crest was reduced, and the creatine and choline wave crest was elevated. This finding was further supported by hematoxylin-eosin staining, which showed a loss of hippocampal neurons and more glial cells. Moreover, electron microscopy showed neuronal shrinkage and mitochondrial rupture, and scanning electron microscopy revealed small size hippocampal synaptic vesicles, incomplete synaptic structure, and reduced number. Overall, the results revealed that 7.0T high-field nuclear magnetic resonance spectroscopy detected the lesions and functional changes in hippocampal neurons of Alzheimer＇s disease rats in vivo, allowing the possibility for assessing the success rate and grading of the amyloid beta （1-40） animal model of Alzheimer＇s disease.

关键词： nerve regeneration Alzheimer's disease Aβ1-40 high-field functional magnetic resonance nuclear magnetic resonance spectroscopy neuropathology N-acetylaspartate creatine choline hippocampus NSFC grant neural regeneration

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Metabolomics profile in gastrointestinal cancers:Update and future perspectives

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World Journal of Gastroenterology 2020年第20期26卷 2514-2532页

作者： Giulia Nannini Gaia Meoni Amedeo Amedei Leonardo Tenori Department of Experimental and Clinical Medicine University of FlorenceFlorence 50134Italy Giotto Biotech Srl and CERM(University of Florence)Florence 50019Italy SOD of Interdisciplinary Internal Medicine Azienda Ospedaliera Universitaria CareggiFlorence 50134Italy Consorzio Interuniversitario Risonanze magnetiche di Metalloproteine Florence 50019Italy

Despite recent progress in diagnosis and therapy,gastrointestinal(GI)cancers remain one of the most important causes of death with a poor prognosis due to late *** tumor markers and detection of occult blood in the stool are the current tests used in the clinic of GI cancers;however,these tests are not useful as diagnostic screening since they have low specificity and low *** that one of the hallmarks of cancer is dysregulated metabolism and metabolomics is an optimal approach to illustrate the metabolic mechanisms that belong to living systems,is now clear that this-omics could open a new way to study *** the last years,nuclear magnetic resonance(NMR)metabolomics has demonstrated to be an optimal approach for diseases’diagnosis nevertheless a few studies focus on the NMR capability to find new biomarkers for early diagnosis of GI *** these reasons in this review,we will give an update on the status of NMR metabolomic studies for the diagnosis and development of GI cancers using biological fluids.

关键词： Metabolomics nuclear magnetic resonance spectroscopy Pancreatic cancer Gastric cancer Colorectal cancer Biological fluids

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NMR spectroscopy: Processing Strategies, Second Updated Edition 2nd updated ed.

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2000年

作者： Dr. Peter Bigler

ISBN: (数字)9783527613427

ISBN: (纸本)9783527299904

Text for the series "Spectroscopic Techniques": Leading software designers and teachers of spectroscopy have pooled their expertise to devise a new series "Spectroscopic Techniques: An Interactive Course". User are able to gain a better understanding of a variety of spectroscopic techniques in these step-by-step guides. Let the experts show you new solutions to practiced problems using software provided on the interactive CD-ROM.

关键词： nuclear magnetic resonance spectroscopy

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NMR Structural Studies on Alamethicin Dimers

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Journal of Southwest Jiaotong University(English Edition) 2003年第2期11卷 172-181页

作者：李星 Environmental Engineering Department Southwest Jiaotong University

N labeled alamethicin dimer was synthesized. The structure and dynamics of alamethicin dimers were studied with nuclear magnetic resonance (NMR) spectroscopy. The data from 15 N-labeled alamethicin dimer suggest little differences in conformation between the dimer and monomer in the Aib 1 -Pro 14 region. Significant difference in the conformation of the C-terminus are manifest in the NH chemical shifts in the Val 15 -Pho 20 region.

关键词： alamethicin dimer nuclear magnetic resonance spectroscopy

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APPLICATION OF FT-RAMAN spectroscopy TO STUDY CHANGES IN CELLULOSE I CRYSTALLINITY THROUGH THE DISSOLVING PULP PROCESS 16th

APPLICATION OF FT-RAMAN SPECTROSCOPY TO STUDY CHANGES IN CEL...

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第十六届木材、纤维及制浆化学国际会议

作者： Viren Chunilall Tamara Bush Forestry and Forest Products Research Centre NRECSIRP.O.Box 17001Congella4013DurbanSouth Africa Department of Chemistry University of KwaZulu-NatalP.O.Box 17001Congella4013DurbanSouth Africa

Cellulose I crystallinity can be defined as the intensity fraction of the crystalline domains in lignocellulosic *** crystallinity can vary significantly de-pending on the origin of

ISBN: (纸本)9787501982066

Cellulose I crystallinity can be defined as the intensity fraction of the crystalline domains in lignocellulosic *** crystallinity can vary significantly de-pending on the origin of

关键词： cellulose I crystallinity FT-Raman spectroscopy NMR spectroscopy Cellulose Spectrography Raman spectrometry iodine Pulp Crystallinity Raman Bleaching Dissolving pulps nuclear magnetic resonance spectroscopy Pinus patula Dissolution

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Serum Metabolome is Altered in Arginine-Supplemented Growing Pigs

Serum Metabolome is Altered in Arginine-Supplemented Growing...

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4th International Symposium on Animal Nutrition,Health and Feed Additive

作者： Q.H.He,X.F.Kong,Z.R.Tang,G.Y.Wu,H.R.Tang,P.P.Ren,F.H. Hao,R.L.Huang,T.J.Li,B.E.Tan,Y.N.Wu,and Y.L.Yin 3 Key Laboratory for Agro-ecological Processes in Subtropical Region,Institute of Subtropical Agriculture,The Chinese Academy of Sciences,Changsha,Hunan 410125,P.R.China 4 National Institute for Nutrition and Food Safety,Chinese CDC,Beijing 100050 China 5 The Graduate School of the Chinese Academy of Science,Beijing 100049,China 6 Department of Animal Science,Texas A&M University,College Station,TX,77843-2471 7 State Key Laboratory of magnetic resonance and Atomic and Molecular Physics,Wuhan Centre for magnetic resonance,Wuhan Institute of Physics and Mathematics,the Chinese Academy of Sciences,Wuhan,Hubei 430071,China

Arginine plays an important role regulating nutrient metabolism,but the underlying mechanisms are largely *** study was conducted to determine the effect of dietary arginine supplementation on the metabolome in serum of growing pigs using H nuclear magnetic resonance spectroscopy. Sixteen pigs（48±4 kg） were randomly assigned to one of 2 groups,representing supplementation with 0 or 1.0%of L-arginine-HCl to corn- and soybean meal-based *** was collected after 46 d *** arginine supplementation decreased fat deposition and increased protein accretion in the body. Principal component analysis showed that serum concentrations of low-density lipoprotein,very-low-density lipoprotein,and urea were lower,but concentrations of creatinine,tricarboxylic-acid-cycle metabolites,alanine,ornithine,lysine and tyrosine were greater in arginine-supplemented than in control pigs. Additionally,the arginine treatment affected serum concentrations of lipid signalling molecules（glycerophosphorylcholine and myo-inositol） and intestinal bacterial metabolites（formate,ethanol,methylamine, dimethylamine,acetate,and propionate）. These novel findings suggest that dietary arginine supplementation alters the catabolism of fat and amino acids in the whole body, enhances protein synthesis in skeletal muscle, and modulates intestinal microbial metabolism in growing pigs.

关键词： arginine metabolomics serum pigs nuclear magnetic resonance spectroscopy

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